番茄穴盤苗組織-N閾值的初步研究

梁 歡， 尚慶茂

(農業部園藝作物生物學與種質創制重點實驗室，中國農業科學院蔬菜花卉研究所，北京 100081)

梁 歡， 尚慶茂*

(農業部園藝作物生物學與種質創制重點實驗室，中國農業科學院蔬菜花卉研究所，北京 100081)

多孔一體式穴盤育苗具有節能、省力、適合機械化操作、生產效率高等顯著特點，是我國蔬菜集約化育苗的主要形式。與傳統營養缽育苗相比，穴盤育苗采用的人工混配輕型基質，通氣孔隙度增大，單株基質容量減小，管理不當極易導致幼苗礦質養分盈虧，實時準確判斷蔬菜穴盤苗養分狀況對壯苗育成至關重要。目前，蔬菜礦質營養診斷方法主要分為培養介質(包括土壤和人工混配基質)分析法和組織分析法[1-2]，介質分析僅能反映根系可從介質獲得的潛在營養水平，分析結果常因取樣方法、貯存條件和養分提取技術而產生偏差。組織分析剔除了介質特性、環境因素對礦質養分吸收的影響，更能直接反映植株礦質營養狀況，研究與應用也更加廣泛。

1 材料與方法

1.1 試驗材料

共采集了番茄17個品種，‘中雜9號’(Zhongza 9)、‘中雜15號’(Zhongza 15)、‘中雜101號’(Zhongza 101)、‘中雜105號’(Zhongza 105)、‘中雜106號’(Zhongza 106)、‘中雜108號’(Zhongza 108)、‘中雜109號’(Zhongza 109)、‘美櫻2號’(Meiying 2)、‘北京櫻桃番茄’(Beijing cherry tomato)來自中國農業科學院蔬菜花卉研究所。‘佳紅4號’(Jiahong 9)、‘佳紅5號’(Jiahong 5)、‘佳粉18號’(Jiafen 18)、‘硬粉8號’(Yingfen 8)、‘仙客1號’(Xianke 1)、‘仙客5號’(Xianke 5)、‘京丹紅小果’(Jingdan red cherry tomato)、‘京丹黃小果’(Jingdan yellow cherry tomato)來自北京京研益農科技發展中心。

化學測定所用試劑均購自國藥集團化學試劑北京有限公司，AR級。

1.2 試驗設計

1.3 測定方法

每重復隨機選取番茄穴盤苗25株，將下胚軸、莖(子葉節至頂端生長點)、各葉位葉柄和葉片分別取樣，剪成1 cm小段(下胚軸、莖、葉柄)或1 cm×1 cm小塊(葉片)，壓蒜器榨取汁液，收集至1.5 mL離心管，4℃冰箱保存備測。

采用Microsoft Excel 2003和SAS 9.2進行統計分析。

2 結果與分析

3 討論

4 結論

表4 番茄穴盤苗不同組織-N閾值

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LIANG Huan, SHANG Qing-mao*

(KeyLaboratoryofBiologyandGeneticImprovementofHorticulturalCrops,MinistryofAgriculture;InstituteofVegetablesandFlowers,ChineseAcademyofAgriculturalSciences,Beijing100081,China)

2014-02-17 接受日期： 2014-10-16 網絡出版日期: 2015-02-04

國家自然科學基金項目(31172001)； 現代農業產業技術體系建設專項資金(CARS-25)； 公益性行業科研專項(201303014)； 中國農業科學院科技創新工程(CAAS-ASTIP-IVFCAAS)資助。

梁歡(1990—)，女，山西臨汾人，碩士研究生，主要從事蔬菜苗期發育調控方面的研究。E-mail：lianghuanconf@126.com * 通信作者 E-mail：shangqingmao@caas.cn

S625.5+4； S641.2

A

1008-505X(2015)03-0709-10