












摘 " "要:【目的】探討白肉枇杷種質資源果實重要性狀的多樣性與相關性,發掘優異資源,以期為創新利用與育種親本選擇提供依據?!痉椒ā恳試引堁坭凌朔N質資源圃(福州)收集保存的白肉枇杷為試材,對單果質量、果實縱徑、橫徑、側徑、果形指數、種子數、單粒種子質量、可溶性固形物含量、可食率等10個果實性狀進行鑒定,并進行多樣性、相關性和主成分分析以及不同來源地差異性分析?!窘Y果】106份白肉枇杷種質資源10個果實性狀的變異系數為9.3%~34.9%,多樣性指數為2.64~2.95。不同來源地白肉枇杷種質資源果實主要性狀存在差異,福建是大果優質白肉枇杷種質資源的主要來源地。相關性分析表明,單果質量與果實縱徑、橫徑、側徑、果肉厚度、種子數、單粒種子質量均呈極顯著正相關,可溶性固形物含量與單果質量、果實縱徑、橫徑、果肉厚度、可食率呈極顯著負相關,可食率與單果質量、果實縱徑、橫徑、側徑、果形指數、果肉厚度呈極顯著正相關;主成分分析表明,第一主成分貢獻率達60.9%,體現果實大小性狀的重要性。評價發掘出綜合性狀優良的種質資源8份,大果的資源3份,高可溶性固形物含量的資源9份,高可食率的資源3份,少核的資源2份?!窘Y論】白肉枇杷種質資源果實性狀多樣性豐富,發掘出不同類型的優異種質資源25份。
關鍵詞:白肉枇杷;種質資源;果實性狀;多樣性;相關分析;主成分分析
中圖分類號:S667.3 文獻標志碼:A 文章編號:1009-9980(2025)01-0082-12
Analysis and evaluation of fruit traits of 106 white-flesh loquat germplasm resources
CHEN Xiuping, JIANG Jimou, DENG Chaojun, XU Qizhi, SU Wenbing
(Fruit Research Institute, Fujian Academy of Agricultural Sciences/Fujian Breeding Engineering Technology Research Center for Longan amp; Loquat, Fuzhou 350013, Fujian, China)
Abstract: 【Objective】 The study aimed to provide basis for efficient exploration and utilization of excellent white-flesh loquat [ Eriobotrya japonica (Thunb.) Lindl.] resources as well as parent selection for breeding new variety through the investigation of diversity and correlation of main fruit traits of white-flesh loquat germplasm resources. 【Methods】 106 white-flesh loquat resources conserved in the National Longan and Loquat Germplasm Resources Nursery (Fuzhou) were used, and ten fruit traits were measured, then coefficient of variation, correlation analysis and principal component analysis were performed among these traits. 【Results】 The coefficient of variation of fruit traits ranged from 9.3% to 34.9% among the investigated white-flesh loquat resources, and the diversity index of these traits ranged from 2.64 to 2.95. The fruit weight ranged from 6.9 g to 64.6 g, the average was 34.7 g and the coefficient variation was 34.9%; the fruit longitudinal diameter ranged from 2.12 cm to 5.95 cm, the average was 4.08 cm and the coefficient variation was 17.1%; the fruit transverse diameter ranged from 2.11 cm to 4.68 cm, the average was 3.77 cm and the coefficient variation was 13.4%; the fruit shape index ranged from 0.86 to 1.38, the average was 1.08 and the coefficient variation was 9.3%; the fruit lateral diameter ranged from 2.07 cm to 4.60 cm, the average was 3.55 cm, and the coefficient variation was 13.8%; the number of seeds per fruit ranged from 1.74 to 6.60, and the average was 3.31, and the coefficient variation was 24.4%; the weight of seed ranged from 0.78 g to 3.26 g, and the average was 2.07 g, and the coefficient variation was 23.7%;the flesh thickness ranged from 3.65 cm to 11.95 cm, the average was 7.87 cm, and the coefficient variation was 18.9%; The soluble solid content ranged from 9.4% to 17.4%, the average was 12.5% and the coefficient variation was 14.1%, the edible rate ranged from 41.5% to 76.5%, the average was 67.0% and the coefficient variation was 9.9%. There was no significant difference in seed number among white-flesh resources of different origin. Moreover, no significant differences in fruit weight, flesh thickness, soluble solids content and edible rate among white-flesh resources from Fujian, Jiangsu, Zhejiang and Guangdong provinces. However, Fujian was the main origin of white-flesh loquat with large fruit and high quality. While Yunnan, Sichuan and Guizhou had few white-flesh loquat resources with small size fruit with thin flesh and low edible rate. The correlation analysis showed that fruit weight was positively correlated with the fruit longitudinal diameter (r=0.90), fruit transverse diameter (r=0.95), fruit lateral diameter (r=0.91), fruit flesh thickness (r=0.79), seed number (r= 0.58), and seed weight (r= 0.63); the content of soluble solids was negatively correlated with the fruit weight (r=-0.46), fruit longitudinal diameter (r=-0.45), fruit transverse diameter (r=-0.49), fruit lateral diameter (r=-0.41), thickness of flesh (r=-0.55) and edible rate (r=-0.56); the edible rate was positively correlated with the fruit weight (r=0.69), fruit longitudinal diameter (r=0.75), fruit transverse diameter (r=0.74), fruit lateral diameter (r=0.69), fruit shape index (r=0.34), weight of seed (r=0.37), and fruit flesh thickness (r=0.80). The principal component analysis (PCA) showed that the contribution rate of the first principal component factor was 60.9%, reflecting the impotance of the fruit size. Twenty-five excellent resources were identified, among these: three accessions with fruit weight higher than 60.0 g, nine accessions with soluble solid content higher than 15.0%, three accessions with edible rate higher than 75.0%, two accessions with less than 2.0 seeds. More excitingly, eight of them showed advantages on fruit weight (≥40.0 g), soluble solid content (≥12.0%) and edible rate (≥68.0%) at the same time compared with the other white-flesh accessions. 【Conclusion】 The fruit traits of the white-flesh loquat resources were abundant, and twenty-five excellent resources of different types of white-flesh were screened out. This study would provide a basis for the exploration and utilization of the white-flesh loquat resources and the parent selection for loquat improvement in the future.
Key words: White-flesh loquat; Germplasm resources; Fruit trait; Diversity; Correlation analysis; Principal component analysis
枇杷是原產我國的特色常綠果樹,有2000多年的栽培歷史,種質資源豐富。枇杷依果肉顏色可分為白肉(白沙枇杷)和紅肉(紅沙枇杷)兩種類型[1],其中白肉枇杷肉質細嫩、汁多味甜、風味良好,深受廣大消費者的青睞。隨著人們生活水平的提高,對高品質白肉枇杷果品的需求也越來越大,“白肉”已成為枇杷育種的重要目標性狀[2-6]。盡管我國白肉枇杷品種豐富,但傳統白肉主栽品種綜合經濟性狀不佳[1-2],如白梨、軟條白沙、白玉等,與傳統紅肉主栽品種相比,普遍存在果小、肉薄、可食率低等問題,已經越來越難以在現代枇杷生產中大面積推廣應用。
種質資源是新品種選育的重要物質基礎,枇杷育種史上的重大突破得益于優異資源的發掘利用[7-8]。果實性狀如單果質量、可食率、可溶性固形物含量等是枇杷的主要經濟性狀,是種質資源利用的重要依據,也是品種選育的重要考量指標。分析果實性狀相關性和遺傳多樣性可以為遺傳育種提供參考,也可以為果實性狀QTL定位提供依據[9-10],對提升育種效率具有重大意義[11]。有關枇杷種質資源的果實性狀如單果質量、可食率、可溶性固形物含量等分析評價已有較多報道[12-21],其中雖涉及少量白肉種質資源,但針對白肉枇杷種質資源果實性狀分析與評價的系統研究還未見報道。筆者所在課題組前期完成了國家龍眼枇杷種質資源圃(福州)中540多份枇杷種質資源果肉顏色鑒定,發掘白肉種質資源106份,本研究繼續開展白肉種質資源果實性狀分析與評價,旨在為優異種質資源的發掘利用與育種親本選擇提供依據。
1 材料和方法
1.1 材料
以國家龍眼枇杷種質資源圃(福州)前期鑒定篩選的106份白肉枇杷種質資源為試材。土壤為砂壤土,管理條件一致。砧木為解放鐘實生苗,生長結果正常,抽穗期疏花穗,不疏果,不套袋。106份白肉枇杷種質資源的來源地包括福建57份、江蘇17份、浙江5份、廣東8份、廣西2份、云南6份、貴州1份、四川2份、重慶1份、江西2份、上海1份,以及國外4份。
1.2 果實性狀測定及評價方法
參照《枇杷種質資源描述規范和數據標準》[22]對單果質量、果實縱徑、果實橫徑、果實側徑、果形指數、果肉厚度、種子數、單粒種子質量、可溶性固形物含量、可食率等性狀進行鑒定。
為保證種質資源果實成熟度一致,取樣由有經驗的試驗人員完成。根據往年經驗結合果皮顏色觀察、可溶性固形物含量測定和現場品嘗,確定每份種質的成熟期[22]。在樹冠外圍中上部中心枝果穗中取10個代表性成熟果實用于性狀觀測。用電子天平(1/100)稱量果實質量、果皮質量、種子質量,用數顯游標卡尺測量果實縱徑、果實橫徑、果實側徑、果肉厚度,用ATAGO手持式數顯折光儀測定果肉可溶性固形物含量(TSS),記錄種子數。計算果形指數、可食率、單粒種子質量,果形指數=果實縱徑/果實橫徑,可食率/%=(單果質量-種子質量-果皮質量)/單果質量×100;單粒種子質量=種子質量/種子數;果實縱徑、橫徑、側徑以cm表示,保留2位小數。每個性狀以2 a(年)正常年份鑒定結果的平均值表示。根據NY/T 2021(農作物優異資源評價規范 枇杷)[23]規定的優異性狀指標及判定方法篩選優異資源。
1.3 數據統計分析
利用Excel2019對數據進行處理,按單果質量10.0 g、果徑(縱徑、橫徑、側徑)0.5 cm、果形指數0.1、可溶性固形物含量1.0%、果肉厚度0.5 cm、可食率5.0%、種子數1.0、單粒種子質量0.5 g的級差對果實性狀進行分級并統計頻數,繪制頻數直方圖。利用DPS 7.05 數據處理系統(Data Processing System 7.05)進行變異系數、多樣性指數、方差分析、相關性分析和主成分分析,變異系數(CV,%)=(S/X)×100,多樣性指數采用Shannon-Weaver信息指數(H′),即H′=-∑PilnPi,Pi為某性狀第i個代碼值出現的頻率,將性狀進行10 級分類,1 級< X-2 S,10級≥X+2 S,中間每級差0.5 S(X為平均值,S為標準差)[24]。剔除份數少的貴州、重慶、上海的種質資源3份,對其余103份種質資源進行不同來源地果實性狀的比較。
2 結果與分析
2.1 白肉枇杷種質資源果實性狀變異分析
從表1可以看出,106份白肉枇杷種質資源單果質量、果實縱徑、果實橫徑等10個果實性狀變異系數為9.3%~34.9%,變異系數從高到低依次為單果質量>種子數>單粒種子質量>果肉厚度>果實縱徑>可溶性固形物含量>果實側徑>果實橫徑>可食率>果形指數,多樣性指數為2.64~2.95。
2.2 果實性狀的頻數分布
2.2.1 " "單果質量 " "單果質量為枇杷果實三大經濟性狀之一,是衡量果實大小的重要指標。106份白肉資源的單果質量為6.9~64.6 g,平均34.7 g,變異系數34.9%。從圖1可以看出,單果質量≥30.0~40.0 g的種質資源最多,34份,占32.1%;其次是≥20.0~30.0 g、≥40.0~50.0 g的資源,各25、23份,各占23.6%、21.7%;≥50.0~60.0 g的資源有9份,占8.5%,均來源于福建;≥60.0 g的種質資源有3份,占2.8%,也來源于福建,分別為PP451、PP282、P350304013;<10.0 g的種質資源4份,來源于四川、云南各2份。
2.2.2 " "果實縱徑、橫徑與側徑 " "106份白肉枇杷種質資源的果實縱徑為2.12~5.95 cm,平均4.08 cm,變異系數17.1%;果實橫徑為2.11~4.68 cm,平均3.77 cm,變異系數13.4%;果實側徑為2.07~4.60 cm,平均3.55 cm,變異系數13.8%。
從圖2可以看出,果實縱徑≥4.0~4.5 cm的種質資源最多,35份,占33.0%;其次是≥3.5~4.0 cm的資源,25份,占23.6%;<3.0 cm的種質資源6份,占5.7%;≥5.0~5.5 cm的種質資源6份,占5.7%;≥5.5 cm的種質資源僅2份,占1.9%。果實橫徑≥3.5~4.0 cm的種質資源最多,55份,占51.9%;其次是≥4.0~4.5 cm的種質資源,25份,占23.6%;<3.0 cm的種質資源有6份,占5.7%;≥4.5 cm的種質資源6份,占5.7%。果實側徑主要分布在≥3.0~4.0 cm,共75份,占70.8%;其次是≥4.0~4.5 cm的種質資源,25份,占23.6%;<2.5 cm的種質資源有5份,占5.1%;≥4.5 cm的種質資源1份,占1.0%。
2.2.3 " "果形指數 " "106份白肉枇杷種質資源的果形指數為0.86~1.38,平均1.08,變異系數9.3%。從圖3可以看出,果形指數≥1.0~1.1的種質資源最多,48份,占45.3%;其次是≥1.1~1.2的種質資源,25份,占23.6%;≥1.3的種質資源5份,占4.7%;<0.9的種質資源極少,僅1份。
2.2.4 " "可溶性固形物含量 " "106份白肉枇杷種質資源的可溶性固形物含量為9.4%~17.4%,平均12.5%,變異系數14.1%。從圖4可以看出,可溶性固形物含量主要分布在≥11.0%~14.0%,共66份,占62.3%;分布在≥14.0%~15.0%的種質資源有9份,占8.5%;≥15.0%的種質資源有9份(占8.5%),來源于廣西2份、四川2份、云南1份、江蘇3份、福建1份,屬高糖種質資源。
2.2.5 " "果肉厚度 " "106份白肉枇杷種質資源的果肉厚度為3.65~11.95 mm,平均7.87 mm,變異系數18.9%。從圖5可以看出,果肉厚度在≥7.5~9.0 mm的種質資源最多,44份,占41.5%;其次是≥6.0~7.5 mm、≥9.0~10.5 mm的種質資源,各29、22份,各占27.4%、20.8%;≥10.5 mm的種質資源2份,占1.9%。
2.2.6 " "可食率 " "106份白肉枇杷種質資源的可食率為41.5%~76.5%,平均67.0%,變異系數9.9%。從圖6可以看出,可食率主要分布在≥65.0%~75.0%,共75份,占70.8%;≥75.0%的種質資源有3份(占2.8%),分別是PP465、白梨3號、P350304013,均來源于福建,為高可食率特異種質資源。
2.2.7 " "種子數 " "106份白肉枇杷種質資源的種子數為1.7~6.6粒,平均3.3粒,變異系數24.4%。從圖7可以看出,種子數3.0~4.0粒的種質資源最多,54份,占50.9%;其次是≥2.0~3.0粒,34份,占32.1%;<2.0粒的種質資源僅2份,占1.9%,分別為PP610、PP180,為少核的特異種質資源。
2.2.8 " "單粒種子質量 " "106份白肉枇杷種質資源的單粒種子質量為0.78~3.26 g,平均2.07 g,變異系數23.7%。從圖8可以看出,單粒種子質量主要分布在1.5~3.0 g,共91份,占85.8%;≥3.0 g或<1.0 g的種質資源較少,各2、1份。
2.3 不同來源地白肉枇杷種質資源果實性狀比較
從表2可以看出,不同來源地白肉枇杷種質資源的單果質量、可溶性固形物含量、果肉厚度、可食率、種子質量等性狀存在顯著差異,種子數差異不顯著。福建的種質資源果實最大,平均單果質量40.4 g,高出總樣本平均單果質量的16.4%,顯著高于江西、云南、四川的種質資源,與廣東、廣西、江蘇、浙江及國外種質資源間的差異不顯著;四川的單粒種子質量最小,顯著小于福建、廣東、廣西、江蘇、江西、浙江及國外的種質資源,與云南的資源差異不顯著;四川、廣西的種質資源可溶性固形物含量較高,顯著高于福建、廣東、江蘇、江西、浙江及國外的種質資源,與云南的資源差異不顯著。福建、江蘇及國外的種質資源果肉較厚,與江西、云南、四川的資源呈顯著差異。浙江、福建及國外的種質資源可食率顯著高于廣西、四川、江西、云南的種質資源,四川的可食率顯著低于除云南外的其他地區。由此可見,福建、廣東、江蘇、浙江及國外的種質資源果大、肉厚、可食率高,但種子大、可溶性固形物含量低;廣西的種質資源果較大、可溶性固形物含量高,但可食率低;四川的種質資源可溶性固形物含量高、種子小,但果小、肉薄、可食率極低。
2.4 白肉枇杷種質資源10個果實性狀的相關性與主成分分析
2.4.1 " "相關性分析 " "果實各性狀間的相關性分析表明(表3),單果質量與果實縱徑、橫徑、側徑、種子數、單粒種子質量、果肉厚度、可食率均呈極顯著正相關;果形指數與單果質量、果實縱徑、可食率呈極顯著正相關;種子數、單粒種子質量與單果質量、果實縱徑、橫徑、側徑、果肉厚度均呈極顯著正相關,單粒種子質量與可食率呈極顯著正相關;可溶性固形物含量與單果質量、果實縱徑、橫徑、側徑、果肉厚度、可食率均呈極顯著負相關,與種子數呈顯著負相關;可食率與果實縱徑、橫徑、側徑、單粒種子質量、果肉厚度均呈極顯著正相關??梢?,在106份白肉枇杷種質資源中,呈現果實越大,可食率越高,種子數也越多、可溶性固形物含量越低的趨勢,果實增大是提高可食率的主要途徑,要選育大果少核或大果高可溶性固形物含量的白肉枇杷品種比較困難。
2.4.2 " "主成分分析 " "主成分分析結果表明(表4),前4個主成分累積貢獻率達92.7%。第1主成分貢獻率為60.9%,特征向量絕對值較大的是果實橫徑、單果質量、果實縱徑、果實側徑,其特征向量都在0.929以上,果肉厚度、可食率也有較大的載荷,代表果實大小因子,可溶性固形物含量有較大的負載荷,與果實大小有逆向作用;第2主成分貢獻率為11.3%,特征向量絕對值較大的是果形指數,其特征向量為0.923;第3主成分貢獻率為11.0%,特征向量絕對值較大的是單粒種子質量、種子數,其特征向量在0.669以上,其中種子數有較大的負載荷,與單粒種子質量有逆向作用;第4主成分貢獻率為9.5%,特征向量絕對值大的是可溶性固形物含量,其特征向量為0.679。
2.5 優異白肉枇杷種質資源的發掘
按照《農作物優異資源評價規范 枇杷》[23]發掘出特異的白肉枇杷種質資源25份,其中單果質量≥60 g的3份、可溶性固形物含量≥15.0%的9份、可食率≥75.0%的3份;兼具單果質量≥40.0 g、可溶性固形物含量≥12.0%、可食率≥68.0%的白肉種質資源8份(表5),果實成熟期集中在4月下旬至5月上旬。
3 討 論
3.1 白肉枇杷種質資源果實性狀的多樣性與相關性
白肉枇杷是肉色為乳白、黃白、黃色等種質資源的統稱,與肉色橙黃、橙紅等紅肉種質資源共同組成枇杷基因資源庫。白肉對紅肉是隱性遺傳[25],白肉枇杷為EjPSY2Ad純合型[26-27],故在自然情況下白肉種質資源數量遠不及紅肉種質資源豐富[1,28-30],但單果質量[14]、可食率[15]等性狀的變異系數與紅肉種質資源相當,在分子水平上也具有豐富的遺傳多樣性[31]。
表型變異是遺傳多樣性和環境多樣性的綜合體現[24,32-33],變異系數能夠直接反映遺傳多樣性[24]。通常認為,變異系數在10%以上,表型性狀變異分化比較明顯[32-33]。張立杰等[17]分析了224份枇杷種質資源單果質量、可溶性固形物含量、可食率等11個果實性狀的變異系數為11.8%~48.9%,變異系數最大的是單果質量,最小的是果形指數。本研究系統分析了白肉枇杷資源果實性狀的多樣性,結果表明,106份白肉枇杷種質資源單果質量、果實縱徑、果實橫徑等10個果實性狀的變異系數為9.3%~34.9%,多樣性指數為2.64~2.95,除了果形指數和可食率,其他性狀的變異系數均在10%以上,說明白肉枇杷種質資源的果實性狀多樣性較豐富,單果質量、果實縱徑、可溶性固形物含量、果實側徑、果實橫徑、可食率、果形指數等性狀的變異系數大小次序與張立杰等[17]的研究結果基本一致,但變異系數均小于前人[11,14-15]的研究結果,尤以單果質量的降幅最大。枇杷F1代單果質量總體呈趨小遺傳的變異趨勢[9,11],因此,在白肉枇杷選育時,宜選擇大果紅肉品種與白肉品種搭配,以提高選育大果白肉品種的概率。
枇杷果實不同性狀間普遍存在相關性[9,17]。本研究表明,單果質量與果實縱徑、果實橫徑、種子數、單粒種子質量、果肉厚度均呈極顯著正相關,可溶性固形物含量與單果質量、果實縱徑、果實橫徑、果肉厚度均呈極顯著負相關,這與前人[9,17]的研究結果一致。主成分分析表明,第1主成分包括果實質量、縱徑、橫徑、側徑等反映果實大小的性狀,與前人[12-13,20]的研究結果基本一致,突顯了果實大小性狀在枇杷分類和利用中的重要作用[20],而可溶性固形物含量與果實大小有逆向作用,說明枇杷的大果和高可溶性固形物含量較難共存[9]。
3.2 不同來源地白肉枇杷種質資源果實性狀的差異
不同來源地枇杷種質資源的單果質量、可食率、果徑等果實性狀存在較大差異[8-12]。本研究發現,不同來源地白肉枇杷種質資源的單果質量、可溶性固形物含量、果肉厚度、種子質量、可食率也存在明顯差異,呈特異性狀區域化分布特點。我國福建、浙江、江蘇、廣東等省份作為枇杷高度進化類型栽培區[1],地區間白肉枇杷種質資源的可溶性固形物含量差異不顯著,果實大小、果肉厚度、可食率等性狀明顯優于其他省份,體現了人們對大果、優質白肉枇杷品種的需求和選擇方向。福建的白肉種質資源數量最多、果實也最大,本研究單果質量≥50 g的12份種質資源及綜合性狀優異的8份種質資源均來源于福建。福建是我國枇杷栽培的主產區,也是我國最大果型枇杷品種解放鐘的原產地,枇杷栽培歷史悠久,經長期的自然變異與人工選擇,形成了豐富的白肉種質資源。而四川、云南、貴州等原生枇杷中心區和外圍區的白肉種質資源不僅數量少,還表現果小、可食率低等特性[1]。本研究中云南的6份白肉種質資源有2份系一果場從浙江引進的枇杷苗木中選出,后經鑒定為軟條白沙類資源;而作為枇杷野生資源豐富區的貴州,也僅1份白肉種質資源(據當地群眾介紹,該份種質資源系早期從江浙引進的苗木中選出,后經鑒定與浙江軟條白沙相似),說明貴州白肉枇杷種質資源稀缺[34]。白肉枇杷種質資源地域分布上的差異,不僅與遺傳基礎有關,還與長期的人為選擇有關。
3.3 白肉枇杷優異種質資源的發掘與創新利用
《農作物優異資源評價規范 枇杷》[23]規定了白肉枇杷優異種質資源單果質量、可溶性固形物含量、可食率、果肉厚度、種子數等性狀的評價指標,為優異種質資源挖掘利用提供了標準規范。本研究通過系統鑒定,從106份白肉枇杷種質資源中發掘出在單果質量、可溶性固形物含量、可食率等單一性狀特異的資源共15份,兼具單果質量、可溶性固形物含量、可食率等綜合性狀優異的種質資源8份,以及少核的種質資源2份(種子數<2)。這些白肉優異種質資源的發掘,為枇杷新品種選育奠定了基礎,已直接創新利用選育出新品種4個,其中貴妃(新白3號)[35]、新白1號(黃蜜)[36]、新白8號通過了國家或省級品種審(認)定,新白2號獲植物新品種權;作為親本材料雜交育成新品種7個,其中福建省農業科學院果樹研究所鄭少泉團隊利用新白2號為父本與早鐘6號[8]雜交育成三月白[37]、白雪早[38]、早白香,利用貴妃(新白3號)作親本雜交育成香妃[8,39]、中白(白早鐘8號)等白肉枇杷新品種,四川省農業科學院園藝研究所利用貴妃(新白3號)為親本材料雜交育成西蜀2號[40]、西蜀3號[3]等枇杷新品種,推動了枇杷產業高質量發展。
4 結 論
106份白肉枇杷種質資源的單果質量、可溶性固形物含量、可食率等10個果實性狀變異系數為9.3%~34.9%,多樣性指數為2.64~2.95,遺傳多樣性豐富。不同來源地白肉枇杷資源單果質量、可溶性固形物含量、果肉厚度、種子質量、可食率等性狀存在顯著差異,福建、江蘇、廣東、浙江及國外的白肉枇杷資源果大、肉厚、可食率高,四川、云南的白肉資源果小、肉薄、可食率低。發掘出不同類型優異種質資源25份,其中單果質量≥60 g的資源3份、可溶性固形物含量≥15.0%的資源9份、可食率≥75.0%的3份,兼具單果質量≥40.0 g、可溶性固形物含量≥12.0%、可食率≥68.0%的白肉資源8份,以及種子數<2粒的資源2份。研究結果為枇杷優異資源的發掘利用和育種親本的選擇提供了理論依據。
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