Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplnio

付冰川(Fu Bingchuan，Dept Plast Surg，NanfangHospSouthernMedUniv，Guangzhou 510515)…∥Chin J Plast Surg.－2010，26(4).－289～294

Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplnio

付冰川(Fu Bingchuan，Dept Plast Surg，NanfangHospSouthernMedUniv，Guangzhou 510515)…∥Chin J Plast Surg.－2010，26(4).－289～294

ObjectiveTo investigate the effect of adipose stromal vascular fraction cells(SVFs)on the survival rate of fat ransplantation.Methods0.5 mL autologous fat tissue was mixed with:①DiI-labeled autologous SVFs(Group A);②DiI-labeled autologous adipose-derived stem cells(ASCs)(Group B);③Complete DMEM(Group C).And then the mixture was injected randomly under the back skin of 14 rabbits.The transplanted fat tissue in three groups was harvested at 6 months after implantation.Wet weight of fat grafts was measured for macroscopic aspects.After HE staining，blood vessel density，viable adipocytes and fibrous proliferation were counted respectively for histological evaluation.Trace of DiI-labeled ASCs in vivo was detected by fluorescent microscope.ResultsThe wet weight of fat grafts in group A(291.0 ± 72.1)mg and group B(269.3 ± 67.3)mg was significantly higher than that in group C(177.8 ±60.0)mg，but the differnce between Group A and Group B was not significant.Histological analysis revealed that the fat grafts in group A and B was consisted predominantly of adipose tissue with less fat necrosis and fibrosis，compared with the fat grafts in group C.The grafts in both group A and B had significantly higher capillary density than those in the control group.Part of vascular endothelial cells were found originating from ectogenic DiI-labeled SVFs and ASCs.ConclusionThe autologous isolated SVFs has a similar effect as autologous cultured ASCs to improve the survival rate of fat transplantation.And the former is more practical and safe，indicating a wide clinical application in the future.16 refs，3 figs.

(Authors)