Expression of bradykinin as a substrate of CD26/DPP IV in rats ischemia/reperfusion injury following lung transplantation

Tang Zheg(唐 政，Dpt Thorac Surg，Union Hosp，Tongji Med Col，Huazhong Univ Sci Technol，Wuhan 430022)…∥Chin J Transplant.－2011，32(7).－426 ～429

Expression of bradykinin as a substrate of CD26/DPP IV in rats ischemia/reperfusion injury following lung transplantation

Tang Zheg(唐 政，Dpt Thorac Surg，Union Hosp，Tongji Med Col，Huazhong Univ Sci Technol，Wuhan 430022)…∥Chin J Transplant.－2011，32(7).－426 ～429

ObjectiveTo investigate the expression of bradykinin as a substrate of CD26/DPP IV in rats with ischemia/reperfusion injury following lung transplantation(LTx).MethodsThirty－six syngeneic male SD rats were randomly allocated into control group and experimental group(n=18 each)，and 36 rats served as donors.In control group and experimental group，the lungs were flush－perfused and preserved with LPD and LPD+CD26/DPP IV catalytic inhibitor(AB192)respectively，and LTx was performed 18 h after cold ischemia.Histopathological studies were also done on the same locus of lung specimens and staining for bradykinin.The different proteins were separated by means of immobilized PH gradient－based two－dimensional gel electrophoresis.The differential expression of bradykinin was compared by Western blotting.ResultsThe expression of bradykinin in control group was significantly higher than in experimental group.As compared with experimental group，bradykinin expression was significantly up－regulated in control group P ＜0.05.ConclusionInhibitor of CD26/DDP 1V enzymatic activity significantly ameliorated ischemia/reperfusion injury in the early stage after LTx，which is probably associated with inhibition of the protein expression of bradykinin.11 refs，4 figs.

(Authors)