Expression of mucin regulated by recombinant human interleukin-6 in HT-29 cell of colon cancer

/Huang Huaming

(黃華明，Dept Gen Surg，1st Affil Hosp Sanxia Univ，Hubei Yichang 430002)…∥Chin J Gastrointest Surg.-2011，14(8).-899 ～902

ObjectiveTo study the regulation of the mucin protein in colon cancer cell line HT-29 by recombinant human interleukin-6(rIL-6)and to further elucidate the development of colon caner.MethodsThe HT-29 cells were treated with different concentrations of rIL-6(1，2，5，10 and 20 μg/L)，then flow cytometry and RT-PCR were used to detect the expression of mucin l and mucin 2.Transwell invasion assay was used to observe the effect of invasion capability of rIL-6 to HT-29 cells.ResultsIn colon cancer，the expression of mucin 1 could be promoted by rIL-6 with concentration above 2 μg/L，the expression rates were(12.5 ± 1.6)%，(26.6 ± 2.7)%，(33.9 ±2.8)%and(58.9 ±2.5)%，respectively，higher than(8.0 ± 0.8)%in the negative controls(P ＜0.01)，meanwhile，the expression of mucin 2 decreased by rIL-6 with concentration above 2 μg/L，the expression rates were(30.5 ±2.6)%，(17.0 ±2.7)%，(11.0 ±2.0)%and(5.3 ± 1.8)%，respectively，lower than(41.6 ± 3.6)%in negative control(P ＜ 0.01).With the increase in rIL-6 concentration，the invasion of HT-29 cells was enhanced.ConclusionIn colon cancer，the expression of mucinl can be promoted by rIL-6，while the expression of mucin2 can be inhibited.IL-6 is a promoting effect factor in colon cancer invasion and metastasis.12 refs，3 figs，2 tabs.