Sensitivity to drugs of chemotherapy of cancer stem cells of human pancreatic carcinoma

/Wang Yuhe

(王育和，Dept Surg，Beijing Xiaotangshan Hosp，Beijing 102211)…∥Chin J Hepatobil Surg.-2011，17(11).-919～923

ObjectiveTo explore the sensitivity of cancer stem cells to chemotherapy in human pancreatic carcinoma.MethodsPANC-1 ceils were cultured in an incubator filled with 5%CO2 at a temperature of 37℃，and were labeled with Hoechst 33342.The SP analysis and sorting were performed using a FACSVantage SE.RTPCR was performed to detect the expression of human CD133 ABCG2 and Notch1.SP and non-SP cells from the PANC-1 cell line were treated with 5-fluorouracil(5-FU;1，10，or 100 μg/ml)or gemcitabine(10，100，or 1000μg/ml)，and the cell viability was determined using the MTT assay.The sensitivity of sorted tumor cells to chemotherapeutics was determined in NOD/SCID mice model.ResultsSP cells were found to have higher drug-resistance both in vivo and in vitro and higher levels of mRNA expression for CD133，ABCG2 and Notch1，when compared to non-SP cells.The xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133± cells than the untreated group.ConclusionThe SP of PANC-1 pancreatic carcinoma cells are enriched with highly gemcitabine-resistant CSCs and determine the carcinogenesis of the PANC-1 pancreatic carcinoma cells.16 refs，4 figs.