212004 Relationship between the single nucleotide polymorphisms in has-mir-125a-5p rs12975333 and expression of has-mir-125a-5p in Han Chinese female breast cancer patients

/Zheng Meizhu(鄭美珠，Dept Breast Surg，Shandong Provin Cancer Res Inst，Jinan 250017)…∥Chin J Gen Surg.-2011，26(7).-596 ～599

ObjectiveTo investigate the relationship between the single nucleotide polymorphisms(SNPs)in has-mir-125a-5p rs12975333 and the expression of has-mir-125a-5p and clinicopathological cheracteristics of female breast cancer in Han Chinese women.MethodsGenomic DNA was extracted from peripheral blood lymphocytes.taqman-MGB assay was used to type breast cancer of 338 cases and 338 controls.Expression levels of has-mir-125a-5p in 289 biopsies were examined using stem-loop real-time RTPCR and the clinicopathological cheracteristics of breast cancer were evaluated.ResultsThe gene frequencies(GG，GT，TT)of rsl2975333 in the patients were GG 273(94.5%)，GT 16(5.5%)，TT 0(0%)，while in breast fibroadenoma and controls there were GG 49(100%)，338(100%).The expression level of hasmir-125a-5p in breast cancer(0.19 ± 0.04)was lower than that in the matched nontumor adjacent tissue specimens(0.37 ±0.05)(P=0.04).The expression level of minor T allele of mature miR-125a in breast cancer patients was lower than that in has-mir-125a-5p-GG carying(P=0.022).The expression of has-mir-125a-5p was down-regulated in primary breast cancer，especially in elder patients(P=0.036)and lymph node metastasis groups(P=0.001)and with negative ERBB2(P=0.007)，ERBB3(P=0.04).Conclusionrs12975333 polymorphisms in has-mir-125a-5p gene may work as a risk factor of breast cancer in Han Chinese women.The altered expression of has-mir-125a-5p might play a role in the pathogenesis and progression of breast carcinoma.12 refs，2 figs，2 tabs.