Study of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptotic and inflammatory gene expressions in colon cancer cell line

/Yu Hongbin

(俞宏斌，Dept Anus Intest，Peop Hosp Huzhou，Zhejiang Huzhou 313000)…∥Chin J Gastrointest Surg.-2011，14(8).-803 ～806

ObjectiveTo study the effect of recombinant tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)on the expression of apoptosis and inflammatory related genes in human colon cancer cell line HCT-116.MethodsAfter 24-hour treatment with recombinant human TRAIL protein，the expressions of apoptosisrelated genes(Bcl-2，Bad，caspase-3，and caspase-8)and inflammation-related genes(TNF-α，IL-1β，and COX-2)were measured by real-time PCR and appropriate kits in HCT-116.ResultsAfter treatments of 10 μg/L and 100 μg/L recombinant human TRAIL proteins，the apoptotic rates of HCT-116 cells were 27.4%and 45.9%，respectively.Expressions of anti-apoptosis gene Bcl-2，pro-apoptosis gene Bad and apoptotic markers caspase-3 and caspase-8were significantly up-regulated，which was more significant in the group of 100μg/L treatment(P＜0.05).Moreover，after TRAIL treatments，expressions of inflammation-related genes TNF-α，IL-1，COX-2were also dramatically increased，and 100 μg/L treatment group showed higher up-regulation(P ＜ 0.05).ConclusionRecombinant TRAIL protein induces both apoptosis and inflammation of human colon cancer cells.7 refs，4 figs，1 tab.