Role of carcinoembryonic antigen and cyclooxygenase-2 in the study of molecule incisal edge for colorectal cancer

/Yang Xiaodong

(楊曉東，Dept Gen Surg，2nd Affil Hosp Soochow Univ，Suzhou 215004)…∥Chin J Gastrointest Surg.-2011，148).-807～809

ObjectiveTo investigate the expression of cyclooxygenase-2(COX-2)and CEA in the tissues adjacent to the tumor within different distances.MethodsA total of 42 colorectal cancer tissues were collected.The adjacent tissues within 3 cm to the tumor were procured every 1 cm.Normal tissue was also collected.RNA was extracted and the expression of CEA and COX-2 was detected by RT-PCR.ResultsThe CEA mRNA levels of the tumor，the tissues of every 1 cm adjacent to the tumor，and the normal tissue were 135.2 ± 23.3，78.2 ± 17.3，75.9 ±16.5，56.2 ±10.7，52.3 ±12.8，18.2 ±7.9，16.2± 6.5，and 16.6 ± 7.0.The levels of COX-2 mRNA in above positions were 134.9 ±31.1，79.2 ±20.2，77.0 ±20.5，62.7 ± 21.9，58.0 ± 18.1，21.2 ± 10.3，18.3 ±7.6，and 17.1 ± 6.3.These data showed a decreasing trend of CEA and COX-2 as the distance increased from the tumor.The CEA mRNA levels showed positive correlation with the levels of COX-2 mRNA(r=0.725，P ＜0.01).ConclusionCEA and COX-2may be considered to be used as biomarkers for the study of molecular resection margin of colorectal cancer.7 refs，2 figs，2 tabs.