視神經損傷的修復與保護機制研究年度報告

徐穎 阮奕文 羅煥敏 陸大祥 鐘敬祥

摘 要：該研究計劃通過分子生物學技術、神經電生理技術、組織化學與免疫組化方法以及視覺行為學檢測方法，研究視神經損傷的病理及修復機制，探討納米材料、神經營養因子、新型Nogo受體抑制劑及抗炎因子等對視神經損傷的修復與保護機制，防治視神經及節細胞的損傷，為視神經假體的成功應用奠定基礎。該年度主要在以下幾個方面開展了研究：（1）探索微電極刺激視神經的安全電流強度范圍、刺激時間時程；（2）測試電極假體插入視神經后的缺血狀態以及缺血對視網膜的影響；（3）探索3，4-二羥基苯甲酸甲酯（MDHB）對氧化應激所致RGC-5細胞凋亡的拮抗作用及其作用機制；（4）探索原花青素對視網膜節細胞的保護作用及其作用機制；（5）探索EphA2基因在視網膜神經節細胞損傷中的作用。

關鍵詞：視神經 神經節細胞 修復與保護

Abstract：This project plans to explore the repair and protection of optic nerve after its damage， through molecular， electrophysiological， histological， immunological and behavioral test. The nana-material， neural nutrition factors， new Nogo receptor inhibitors and anti-inflammation factors will be applied to test their effects on the protection of optic nerve， in order to prevent the optic nerve damage and the death of retinal ganglion cells. The ultimate object is to provide foundation for the clinical application of optical nerve prosthesis. In this year， we developed the following researches：（1）Explored the safe range of the electric intensity and stimulating duration of micro-electrode inserted into the optic nerve.（2）Explored whether the insertion of micro-electrode would cause ischemia in optic nerve and how temporal ischemia in the brain affected the histology and function of retinal neurons and the visual behavior of the animal.（3）Explored the protection effect of MDHB on oxidative-stress injured RGC-5 cells and its mechanism.（4）Explored the protection effect of oligomeric proanthocyanidin on retinal ganglion cells against oxidative stress induced apoptosis in vitro.（5）Explored the function of EphA2 in the protection of retinal ganglion cells.

Key Words：Optic nerve； Retinal ganglion cell； Repair and protection

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