獨角蓮軟膠囊抗人肝癌Hep—2荷瘤裸鼠移植瘤作用及對p53表達的影響

段玉敏 張洪娟 劉濱瑞 孟慶杰 劉微

摘要：目的 觀察獨角蓮軟膠囊對Hep-2荷瘤裸鼠腫瘤生長的抑制作用及對抑癌基因p53表達的影響，探討其作用機制。方法 背部皮下接種人肝癌Hep-2細胞懸液制備腫瘤模型。BALB/c裸鼠隨機分為模型組、陽性藥組和中藥高、中、低劑量組，各給藥組給予相應藥物灌胃，1次/d，連續21 d。監測瘤體積變化，HE染色觀察各組瘤組織病理變化，RT-PCR檢測各組Hep-2荷瘤裸鼠腫瘤組織p53基因的表達。結果 與模型組比較，各給藥組均能抑制腫瘤生長，抑瘤率分別為55.1%、42.8%、30.1%、79.5%，p53基因表達上調；病理觀察結果顯示，模型組腫瘤細胞數目增多、體積增大、胞質疏松、核深染，各給藥組均有不同程度的核固縮、淡染、毛細血管數目減少和局灶性壞死。結論 獨角蓮軟膠囊具有抑制人肝癌生長作用，其機制可能與上調p53基因的表達導致細胞凋亡有關。

關鍵詞：獨角蓮軟膠囊；抗腫瘤；p53基因表達；裸鼠

DOI：10.3969/j.issn.1005-5304.2016.07.016

中圖分類號：R285.5 文獻標識碼：A 文章編號：1005-5304（2016）07-0061-04

Inhibitory Effects of Typhonium Giganteum Soft Capsules on Transplantation Tumor and Expression of p53 Gene in Human Liver Cancer Hep-2 Tumor-bearing Nude Mice DUAN Yu-min1， 2， ZHANG Hong-juan2， LIU Bin-rui2， MENG Qing-jie2， LIU Wei2 （1. Shenyang Normal University， Shenyang 110000， China； 2. Heilongjiang Academy of Traditional Chinese Medicine， Harbin 150030， China）

Abstract： Objective To observe the inhibitory effects of Typhonium Giganteum soft capsules on tumor growth in Hep-2 tumor-bearing nude mice and its effects on the expression of tumor suppressor p53 gene； To discuss its mechanism of action. Methods Human liver cancer Hep-2 cell suspension back subcutaneous vaccination was conducted to prepare tumor models. BALB/c nude mice were randomly divided into model group， cisplatinum group， and TCM high-， medium-， and low-dose groups. Each group was given relevant medicine for gavage， once a day for 21 days. Growth changes of tumor volume were monitored； HE staining was used to observe pathological changes of tumor tissues； RT-PCR was used to detect the expression of p53 gene in tumor tissue of Hep-2 nude mice. Results Compared with the model group， all medication groups could inhibit the tumor growth， and the anti-tumor rates were 55.1%， 42.8%， 30.1%， and 79.5%， respectively； the expression of p53 gene increased； pathological observation results showed that the number and the volume of tumor cells increased， with cytoplasm rarefaction and nucleus anachromasis. All medication groups had karyopyknosis， slight staining， and decreasing blood capillary and focal necrosis in varying degrees. Conclusion Typhonium Giganteum soft capsules can inhibit the growth of human liver cancer， and its mechanism may be associated with the up-regulating expression of p53 gene leading to apoptosis.

Key words： Typhonium Giganteum soft capsules； anti-tumor； expression of p53 gene； nude mice

我國肝癌死亡率位居世界第2，男性發病率明顯高于女性[1]。目前肝癌患者中大部分失去手術指征，肝功能異常并伴有機體其他癥狀，對放化療的敏感性