High-definition optical magnification with digital chromoendoscopy detects gastric mucosal changes in dyspeptic-patients

Carlos Robles-Medranda,Manuel Valero,Miguel Puga-Tejada,Roberto Oleas,Jorge Baquerizo-Burgos,Miguel Soria-Alcívar,Haydee Alvarado-Escobar,Hannah Pitanga-Lukashok

Carlos Robles-Medranda,Manuel Valero,Miguel Puga-Tejada,Roberto Oleas,Jorge Baquerizo-Burgos,Miguel Soria-Alcívar,Haydee Alvarado-Escobar,Hannah Pitanga-Lukashok,Gastroenterology and Endoscopy Division,Instituto Ecuatoriano de Enfermedades Digestivas(IECED),Guayaquil 090505,Ecuador

Abstract

Key words:Gastritis;Helicobacter pylori;Gastric mucosa;Atrophic;Endoscopy;Digestive system

INTRODUCTION

Gastric cancer is the second most common cause of cancer-related death worldwide,and upper endoscopy is essential to enable its identification at an early stage.() is involved in the pathogenesis of gastric diseases,such as peptic ulcers,gastric lymphoma,and gastric cancer.Thus,it is important to detectinfection and premalignant gastric lesions at an early stage so that the best therapeutic approaches can be offered.Diagnosis of-associated gastritis and gastric atrophy on the basis of endoscopic findings can be difficult,so histology is considered to be the diagnostic gold standard for these conditions.infection and the resultant gastric atrophy are known premalignant lesions linked to gastric carcinogenesis.However,the reliability of detection ofinfection or gastric atrophy by histology depends on the location,number,and size of the endoscopy-guided gastric-biopsy specimens.Alternatively,obtaining samples for histological assessment by endoscopic gastric biopsy can result in significant sampling errors.

Pentax Medical (HOYA,Tokyo,Japan) developed the Optical Enhancement? (OE)System,which combines bandwidth-limited light with an endoscopy video system.This technology combines digital signal processing (similar to I-SCAN?) with optical filters that limit the spectral characteristics of the illuminating light,enhancing visualization of the mucosal surface and microvessels.In addition,MagniView?endoscopes have been developed,and can combine high-definition imaging with optical magnification.

The present study was designed to enable evaluation of the OE System with optical magnification for diagnosis of normal gastric mucosa,-associated gastritis,and gastric atrophy.Additionally,interobserver and intraobserver reproducibility in the assessment of endoscopic patterns detected was assessed.

MATERIALS AND METHODS

Study design

The investigation involved a cross-sectional,nonrandomized,double-blind study that was performed at the Instituto Ecuatoriano de Enfermedades Digestivas,Academic Tertiary Center,Ecuador,between November 2015 and April 2016.The study protocol and consent form were approved by the Institutional Review Board,registered at ClinicalTrials.gov (ID:NCT02597517),and the study was conducted according to the guidelines of the Declaration of Helsinki.All patients provided written informed consent.All authors had access to the study data and had reviewed and approved the final manuscript.

Population selection

The required sample size was estimated with a 95% confidence interval (CI) and a 7.5% margin of error,on the basis of the results of Tongtawee.All consecutive participants had functional dyspepsia according to the Rome III criteria,and were ≥18 years old.Participants had an epigastric pain syndrome (defined as localized pain or burning pain in the upper abdomen at least once a week,which was intermittent,nongeneralized,not relieved by defecation,and did not meet the criteria for pathology of the gallbladder or sphincter of Oddi) and/or a postprandial distress syndrome (defined as the presence of a nagging feeling of postprandial fullness after normal-volume meals,and/or early satiety that prevented the completion of a regular meal several times a week).The criteria had to be present within the three months prior to enrolment,and to have started ≥ 6 mo prior to diagnosis of dyspepsia.Patients with severe uncontrolled coagulopathy,prior history of gastric surgery,or ongoing pregnancy,as well as patients who had received nonsteroidal antiinflammatory drugs (NSAIDs),proton-pump inhibitors (PPIs) or antibiotics in the preceding three weeks were excluded.

Participants were tested forinfection with thestool antigen test(Wondfo,Wondfo Biotech Co.,Guangzhou,China),prior to allocation into two groups:and(control group) (Figure1).Finally,upper endoscopy was performed with the OE System and optical magnification.Endoscopists and participants were blinded to the group allocation.

Endoscopic technique

Complete endoscopic procedures were performed,with evaluation of the entire stomach with conventional white light,to exclude obvious lesions.Participants were also evaluated by upper endoscopy with the OE System (including OPTIVISTA EPK-i7010 HD Video Processor;Pentax Medical,Hoya Corp.,Japan) and MagniView?EG-2990Zi Video Gastroscope (Pentax Medical,Hoya Corp.,Japan) under intravenous sedation in a standardized manner.This technique involved the use of a distal black rubber hood (OE-A58;Pentax) at the tip of the endoscope,to fix the distance between the tip of the endoscope and the gastric mucosa at 2 mm.The OE System was initially used in mode 1 and mode 2 without optical magnification,to obtain an overview of the gastric body and identify any gross changes in the mucosa,then optical magnification was implemented.At maximum magnification,the hood was brought into contact with the gastric mucosa,and water was instilled.Any residue in the stomach was removed with a water-ejection pump prior to the procedure.Each endoscopy was performed by one of three endoscopists (Robles-Medranda C,Valero M,and Soria-Alcívar M),who were assigned by randomized allocation,blinded to the group selection,and trained in the use of the OE system with optical magnification.

OE System and MagniView? endoscope

OE System:The OE System combines digital signal processing with optical filters that limit the spectral characteristics of the illuminating light,connecting the peaks of the hemoglobin absorption spectrum (415 nm,540 nm,and 570 nm) to create a continuous wavelength spectrum.The OE System has two modes that use different filters to optimize visualization of specific features.Here,only mode 1 was used for the highmagnification studies,because of its ability to enhance microvessel visualization.

MagniView endoscope:The MagniView? combines a high-definition endoscope with optical magnification,to produce detailed images with magnification of up to 136×.This imaging facilitates the evaluation of the superficial vascular aspects of the mucosa,enabling identification of early signs of inflammation or lesions not previously seen with conventional endoscopy.

Figure1 Flowchart showing the process of classification of the study participants.NSAIDs:Nonsteroidal antiinflammatory drugs;PPIs:Proton-pump inhibitors;Hp:Helicobacter pylori.

Microvascular-architecture patterns

The gastric body was chosen instead of the antrum for evaluation of the mucosa.The microvascular architecture of the normal stomach shows two distinct patterns depending on the region of the stomach.The gastric body has a honeycomb-like subepithelial capillary network (SECN) pattern with collecting venules,whereas the gastric antrum has a coil-shaped SECN pattern,where collecting venules lie in deeper layers and cannot be seen.

Endoscopic evaluation enabled classification of participants according to four patterns of microvascular architecture,on the basis of the combination of the SECN,collecting venules,and round pits.The type I pattern consisted of a honeycombtype SECN with a regular arrangement of collecting venules (RAC) and regular round pits;type IIa involved a honeycomb-type SECN with regular round pits,but with loss of collecting venules;the type IIb pattern consisted of enlarged white pits surrounded by erythema with loss of normal SECN and collecting venules;and the type III pattern involved loss of normal SECN and round pits,with irregular arrangements of collecting venules (Figure2).

Endoscopic images were recorded,and biopsy samples were obtained to correlate the images with histological assessments.The biopsies were taken with regular biopsy forceps at random locations following the Sydney protocol (two from the gastric body,two from the antrum and one from the incisura angularis) in cases of type I pattern,with additional targeted biopsies from areas with type IIa,IIb,or III patterns.The specimens were fixed immediately in 10% formalin solution,stained with hematoxylin-eosin for histopathological assessment,and stained with the Giemsa stain fordetection.Detection ofinfection was performed by histology and by thestool antigen test.A positive result with either of these tests was considered to indicateinfection.The pathologist was blinded to the endoscopic diagnosis.

Figure2 Four microvascular patterns identified by endoscopy of the gastric body mucosa.A:The type I pattern comprises a honeycomb-type subepithelial capillary network (SECN),with a regular arrangement of collecting venules and regular,round pits;B:The type IIa pattern comprises a honeycomb-type SECN with regular,round pits,but with loss of collecting venules;C:In the type IIb pattern,there is loss of the normal SECN and collecting venules,and the presence instead of enlarged white pits surrounded by erythema;D:The type III pattern is characterized by loss of the normal SECN and round pits,with irregular arrangement of the collecting venules.

Interobserver and intraobserver agreement

A dataset containing 60 photographs taking during the study of the gastric body was presented in a blinded manner to four endoscopists who were individually asked to classify the photographs according to the four microvascular patterns at three time points,each 1 wk apart.At each evaluation,the same photographs were shown to the endoscopists in a different order.The four endoscopists were trained to evaluate the four patterns.Interobserver agreement was assessed by comparison of the photographic analyses by each endoscopist (Alvarado-Escobar H,Puga-Tejada M,Oleas R,and Baquerizo-Burgos J),and intraobserver agreement was assessed by comparison of the photographic analysis by each endoscopist at each time point.

Statistical analysis

The statistical review of the study was performed by a biomedical statistician.The baseline characteristics ofandpatients were compared by Pearson's chisquare or Fisher's exact tests for categorical variables and the Mann-Whitney-test for continuous variables.Continuous variables are expressed as the mean (standard deviation) or median (interquartile range),according to their statistical distribution.Categorical variables are presented as percentages.The sensitivity,specificity,predictive values,and accuracy of the endoscopic findings for normal gastric mucosa,infection,and gastric atrophy were calculated with 95% CIs.For interobserver and intraobserver agreement,kappa values were calculated.Kappa coefficients ＜ 0.4 indicated “poor agreement”,values of 0.4-0.8 represented“moderate-to-good agreement”,and values ＞ 0.8 indicated “excellent agreement”.Avalue ＜ 0.05 was considered statistically significant.Data analysis was performed using R v3.4.3 (R Foundation for Statistical Computing,Vienna,Austria).

RESULTS

The sample-size calculation indicated that the study required at least 68 patients.Of the 72 participants who were enrolled,35 (48.6%) were dyspepticand 37 (51.4%)were dyspeptic.Histopathology and thestool antigen test showed 100%agreement for diagnosis ofinfection.There were no significant differences betweenandgroups in terms of age (mean 46.3 ± 13.7 years),sex (69.4%female),or primary symptom (58.3% epigastric pain) (Table1).

Endoscopic images were analyzed and patients were classified following agreement among endoscopists (Robles-Medranda C,Valero M,and Soria-Alcívar M),into type I (30.6%),type II 55.6%,and type III (13.9%).We compared distribution ofpatients according to the microvascular types with distribution according to histopathology and-infection results.Among the 10 individuals with normal mucosal histology,90% were type I (Table2).The type I pattern was also present in three individuals with chronic active gastritis and 10 with chronic inactive gastritis.Most individuals with type IIa and type IIb patterns (10 out of 13 and 21 out of 27,respectively) had chronic active gastritis,and similar numbers were.Notably,32 of the 35individuals (91.5%) were Type IIa-IIb.Only three individuals had gastric atrophy identified by histology;one with type IIb microvascular pattern and two (66.7%) with type III pattern.Eight of the 10 individuals with a type III pattern had chronic inactive gastritis,and two exhibited gastric atrophy,but none of them were.

Table1 Baseline demographic data of study participants,who had functional dyspepsia,according to Helicobacter pylori infection status

Predictive performance of the microvascular patterns was calculated (Table3).The type I pattern was predictive of normal mucosa,with sensitivity of 90.0% and accuracy of 80.5%.The presence of a type IIa or type IIb pattern was predictive ofinfection,with sensitivity of 91.4% and accuracy of 84.7%.The type III pattern predicted gastric atrophy with a sensitivity 66.7% and accuracy of 87.5%.For assessment of the three endoscopic patterns,interobserver agreement had a kappa value of 0.89 (95%CI:0.84-0.93) and intraobserver agreement had a kappa value of 0.91 (95%CI:0.85-0.96).

DISCUSSION

In the present study of individuals with dyspepsia,90% of those with histologically normal gastric mucosa had the type I microvascular pattern on endoscopy and were,91.5% ofparticipants had the type IIa or type IIb pattern,and of the three individuals with gastric atrophy on histology,two had the type III pattern on endoscopy.Identification of these microvascular patterns with the OE System with optical magnification enabled prediction of normal gastric mucosa orassociated gastritis with high sensitivity and accuracy,with excellent interobserver and intraobserver agreement.

In recent decades,the role of upper endoscopy in the real-time identification ofinfection of the stomach and gastric atrophy has been evaluated.Initially,studies that were designed to determine whether there was a relationship between endoscopic features and-induced gastritis used white-light endoscopy.The results of these studies showed that some endoscopic features related toinfection were difficult to distinguish from non-gastritis and were not specific.Additionally,prediction of gastric atrophy through endoscopic signs such as the absence of gastric folds and the presence of visible vessels had low sensitivity(67%) and specificity (48%).These results suggested thatinfection and gastric atrophy can be suspected,but not confirmed,by endoscopy alone,and that histology is needed for a definitive diagnosis.

The reliability of detection ofinfection or gastric atrophy by histology depends on the location,number,and size of the gastric biopsies,but considerable sampling errors can occur during endoscopic biopsy sampling.Advances inmagnification technology have made it possible to determine endoscopically whether patients haveinfection.The blood-vessel network in the surface layer of the gastric body has been shown endoscopically to be associated with previous histopathology findings.Normal gastric-body mucosal microvascular architecture consists of a honeycomb-type SECN and collecting venules in a regular arrangement.The presence and regular distribution of numerous red spots(collecting venules) in the gastric body have been shown to indicate a stomach with noinfection.The RAC as an endoscopic feature has been shown to have sensitivity,specificity,and accuracy for identification of anstomach of 93.8%,96.2%,and 95.5%,respectively,with RAC-negative findings having an accuracy of 95% for identification ofinfection.In the current study,RAC positivity (a type I microvascular pattern) had a sensitivity,specificity,and accuracy to predictof 90%,79%,and 80.5%,respectively,and RAC negativity (a type IIa or type IIb pattern) had a sensitivity,specificity,and accuracy to predictof 91.4%,78.3%,and 84.7%,respectively.

Table2 Distribution of microvascular-pattern types according to histopathology and Helicobacter pylori infection

Nakagawaevaluated the association of patterns of collecting venules(regular,irregular,or obscured) withinfection and histopathological gastritis.The presence of a regular pattern of collecting venules in the gastric mucosa was shown to indicate an absence ofinfection,which suggests that a biopsy is unnecessary.Conversely,observation of an obscure or irregular pattern was shown to indicateinfection,with the irregular pattern suggesting the presence of severe gastric mucosal atrophy.

The endoscopic classification that we used was based on a previous evaluation of the gastric body by magnified white-light endoscopy.Here,the use of the OE System with optical magnification improved the identification of mucosal superficial and vascular patterns and made it easier to classify images,thereby improving interobserver agreement (0.890.73).In the previous study,type I and type II/III sensitivities for prediction of normal mucosa andrelated gastritis (92.7% and 100%) were similar to our findings (90% and 91.4% for type IIa and IIb,respectively).However,the type IV pattern sensitivity for prediction of gastric atrophy was 90%with white-light endoscopy compared with 66% (type III) with the OE System,although this difference could be explained by the greater number of individuals with the type IV pattern in the previous study compared with the current study (2110).

The present investigation had some limitations.First,the use of the magnified OE System with optical magnification for these indications has not been studied systematically,and there is a learning curve in obtaining and interpreting the images.This was a small,single-center study,and further investigations with multiple endoscopists are needed.Neither the severity of gastritis nor the degree of endoscopic gastric mucosal atrophy was evaluated.The sample size of patients with gastric atrophy was small.Finally,intake of NSAIDs and PPIs in the three weeks preceding the study was considered an exclusion criterion in our study,to avoid bias and an incorrect interpretation.

Our results demonstrate the potential of the OE System with optical magnification to enable the identification of histopathological changes in the gastric mucosa.In this study,the reproducibility of the classification was excellent.High-definition optical magnification and digital chromoendoscopy exhibited high accuracy for the diagnosis of normal gastric mucosa and-associated gastritis,with lower accuracy for the evaluation of gastric atrophy.Clinical practice experience needs to be further tested in order to determine the applicability of these techniques in defining chronicactive gastritis andinfection.In order to validate our results,the optical enhancement with magnification technologies should be evaluated in randomized,controlled trials.In addition,further studies are needed to determine whether endoscopic diagnosis of gastric atrophy is feasible.

Table3 Predictive performance of the endoscopic microvascular patterns

ARTICLE HIGHLIGHTS

Research background

Helicobacter pylori (H.pylori) infection and atrophic gastritis are linked to gastric carcinogenesis.The endoscopic diagnosis of H.pylori infection and gastric atrophy is challenging,requiring histological confirmation; however,sampling errors might occur.

Research motivation

Digital chromoendoscopy with optical magnification improved the identification of mucosal superficial and vascular patterns in the gastric mucosa and might provide a more accurate endoscopic visual impression of the gastric mucosa.

Research objectives

The main objective of the study was to evaluate digital chromoendoscopy with optical magnification for the diagnosis of normal gastric mucosa,H.pylori associated gastritis and atrophic gastritis.

Research methods

This was a cross-sectional,nonrandomized,single-center study in which consecutive patients with functional dyspepsia were evaluated via esophagogastroduodenoscopy using a high definition endoscope with digital chromoendoscopy and optical magnification for the endoscopic diagnosis of H.pylori associated gastritis and atrophic gastritis.The endoscopic visual impression was compared to H.pylori stool antigen test and histological analysis.

Research results

We described a high sensitivity and accuracy for predicting normal gastric mucosa and H.pylori associated gastritis,with a high inter and intraobserver agreement.Atrophic gastritis was detected with a low sensitivity,and further studies are required to determine if endoscopic diagnosis of atrophic gastritis is feasible.

Research conclusions

In our study,esophagogastroduodenoscopy with digital chromoendoscopy and optical magnification enable the identification of histological changes in the gastric mucosa of consecutive patients with functional dyspepsia.

Research perspectives

We encourage a randomized multicenter trial evaluating high definition white light endoscopy versus digital chromoendoscopy with optical magnification for the evaluation of the gastric mucosa of dyspeptic patients, in order to determine the clinical practice applicability of these techniques. Further studies are needed to determine if endoscopic diagnosis of atrophic gastritis is feasible.