Venetoclax in combination with chidamide and dexamethasone in relapsed/refractory primary plasma cell leukemia without t(11;14): A case report

Yang Yang,Li-Juan Fu,Chun-Mei Chen,Mei-Wei Hu

Yang Yang,Li-Juan Fu,Chun-Mei Chen,Mei-Wei Hu,Department of Hematology,The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310014,Zhejiang Province,China

Abstract BACKGROUND Conventional therapies for primary plasma cell leukemia (pPCL) are usually ineffective,with a short remission time with the use of multiple myeloma medications,showing aggressiveness of pPCL.B-cell lymphoma-2 inhibitor venetoclax is usually used for relapsed/refractory multiple myeloma (RRMM)with t(11;14).There are very few studies published on the use of venetoclax in pPCL without t(11;14).Similarly,histone deacetylase inhibitors are considered effective for the treatment of RRMM,but there are no reports on their use in pPCL.CASE SUMMARY A 57-year-old woman with severe anemia,thrombocytopenia,multiple bone destruction,impaired renal function,and 42.7% of peripheral plasma cells is reported.After multiple chemotherapy regimens and chimeric antigen receptor Tcell treatment,the disease progressed again.The patient had very good partial response and was maintained for a long time on venetoclax in combination with chidamide and dexamethasone therapy.CONCLUSION The success of venetoclax-chidamide-dexamethasone combination therapy in achieving a very good partial response suggested that it can be used for refractory/relapsed pPCL patients who have been exhausted with the use of various drug combinations and had poor survival outcomes.

Key Words: Relapsed/refractory; Primary plasma cell leukemia; Venetoclax; Chidamide;Very good partial response; Case report

INTRODUCTION

Plasma cell leukemia (PCL) is a rare and highly aggressive form of plasma cell dyscrasia and is characterized by the presence of more than 20% and/or more than 2.0× 109/L of circulating plasma cells (PCs) in the peripheral blood.PCL is classified as primary (p)PCL when the leukemic phase is present at the time of diagnosis and as secondary (s)PCL when it is previously diagnosed with multiple myeloma (MM).At present,pPCL accounts for approximately 50% of PCL cases[1].Patients with pPCL are generally younger than those with sPCL (median age 55 yearsvs66 years).The characteristic pattern of PCL revealed PCL and MM as different diseases,not only clinically but also genetically[2].Immunoglobulin H translocations in t(11;14) were observed in pPCL (33%-63%),where it predicts its sensibility to B-cell lymphoma-2(BCL-2) inhibitor[3].The prognosis of pPCL remains usually very poor,and the median overall survival is reported to be less than 1 year[4].Both BCL-2 inhibitor and histone deacetylase inhibitors (HDACIs) have curative effects on relapsed/refractory multiple myeloma but are limited when used in pPCL.We herein reported a combination regimen of venetoclax-daratumumab-chidamide that led to an unexpected rapid and very deep hematologic remission in a relapsed pPCL patient.To our knowledge,this is the first case report in a pPCL patient without translocation t(11;14).

CASE PRESENTATION

Chief complaints

Pain in the lumbar spine and fatigue.

History of present illness

A 57-year-old woman with a 1 mo history of waist pain and fatigue visited the Orthopedics Department.Examination of peripheral blood revealed white blood cell count of 22.6 × 109/μL with 49% PCs,hemoglobin of 3.2 mg/dL,and a platelet count of 37000/μL.She was then transferred to our department.

History of past illness

The patient had rib fracture caused by trauma and anemia before 7 mo.

Personal and family history

No data were available.

Physical examination

Physical examination revealed severe anemia and tenderness of the middle and lower sternum.Local tenderness at 2-3 lumbar vertebrae,and leg elevation test showed negative for both lower limbs.

Laboratory examinations

The patient at the time of joining the hospital had renal function damage (serum creatinine 188.9 μmol/L) and hypercalcemia (serum calcium 2.81 mmol/L).Serum and urine immunofixation electrophoresis revealed only lambda light chain monoclonal antibodies,and the serum lambda light chain levels were found to be significantly increased (2.80 g/L; normal range,0.90-2.10 g/L).β-2 microglobulin levels were also found to be increased (12.84 mg/L; normal range,1.00-3.00 mg/L).

Imaging examinations

A computed tomography scan showed multiple destruction of bones (thoracic spine,lumbar spine,ribs,etc.).

Further diagnostic work-up

Peripheral smear showed 49% PCs in June 2015 (Figure 1).Flow cytometry (FCM)analysis confirmed that 42.7% of PCs in the peripheral blood were positive for CD38,CD138,CD56,and cytoplasmic immunoglobulin λ (Figure 2A).Bone marrow analysis also revealed that the immature-like PCs in the smear were increased to 70.5%,which was consistent with that in the peripheral blood through FCM analysis.Pathological analysis of bone marrow biopsy revealed that the morphology of abnormal PCs was similar to that of bone marrow images,and immunohistochemistry results revealed that CD38 and CD138 were positive (Figure 3).No analyzable cleavage phase was seen in conventional chromosomes.Fluorescencein situhybridization (FISH) analysis revealed deletion of 13q14 and amplification of 1q21,but the frequency of p17 deletion and t(11;14) were within the normal range (Figure 4A and B).Therefore,the patient was diagnosed with pPCL (λ light chain type).

FINAL DIAGNOSIS

Primary plasma cell leukemia.

TREATMENT

The patient was initially treated with bortezomib,thalidomide,cyclophosphamide,and dexamethasone for two cycles,and then thalidomide was changed to lenalidomide after two cycles.After treatment,the patient achieved very good partial response (VGPR) by normalizing thrombocytopenia and anemia,and achieving near resolution of her bone marrow plasmacytosis (0.8% of monoclonal PCs) and persistent positive serum immunofixation after four cycles.Autologous hemopoietic stem cell transplantation was performed,but the collection of stem cells failed.The patient refused to undergo allogeneic hematopoietic stem cell transplantation.Bortezomib,lenalidomide,cyclophosphamide,and dexamethasone (VRCD) was continued,and her condition was evaluated as complete response with negative serum immunofixation after six cycles of chemotherapy,but her FISH test was shown to be negative after eight cycles of chemotherapy.The patient’s treatment-related side effects were incomplete intestinal obstruction and mild diarrhea.A total of 10 cycles of chemotherapy were completed,and the regimens of bortezomib,lenalidomide,dexamethasone/lenalidomide,and dexamethasone (VRD/RD) were given as alternate maintenance treatment.Considering the side effects of bortezomib,VRD was used every 3 mo.

After maintenance treatment for nearly 7 mo,she experienced a biochemical relapse,wherein the bone marrow was infiltrated by 2.84% of PCs.She underwent salvage therapy with four cycles of VRCD again,and her curative effect was evaluated as stable disease.In July 2017,her treatment was switched to isazomib,lenalidomide,cyclophosphamide,and dexamethasone (IRCD),achieving a complete response with serological and urine of negative immunofixation and less than 0.01% of monoclonal PCs in the bone marrow.IRCD was given as the main treatment program for 10 cycles,but the disease progressed again.She was then immediately given B cell maturation antigen and CD269 chimeric antigen receptor T-cell immunotherapy (CAR-T) in July 2018,and the remission lasted for more than 1 year after treatment.After more than 1 year of CAR-T treatment,minor residual disease showed progressive increase.

Figure 1 Circulating plasma cells as evident on the peripheral smear (1000 ×) in June 2015.

In October 2019,she started using daratumumab,lenalidomide,and dexamethasone chemotherapy.After 6 wk,her platelet count was shown to be rapidly declined,the residual disease level as assessed by FCM was 48.29%,and her FISH test remained the same as that when the disease was first diagnosed,indicating that the disease was still in the progressive stage.Selinexor [exportin 1 (XPO1) inhibitor]/dexamethasone regimen was then given,and the regimen was rechecked after two cycles,but the treatment regimen failed to achieve control,with approximately 42.2% monoclonal PCs in her peripheral blood immunophenotyping (Figure 2B).So,the regimen was considered invalid.Because there was limited access to carfilzomi or pomalimidone in China,salvage immunotherapy including venetoclax in combination with chidaniline and dexamethasone (chidaniline 20 mg twice a week,venetoclax 300 mg/d,dexamethasone 20 mg once a week) was given for 28 d (i.e.for one cycle).Reassessment after two cycles revealed progressive decline in her peripheral PCs,the bone marrow had 0.5% of PCs,and bone marrow FCM identified 0.1% of clonal PCs(Figure 2C).A summary report of clinical and treatment assessments are presented in Table 1.

OUTCOME AND FOLLOW-UP

The patient experienced the deepest response of VGPR after four cycles.Currently,the patient is still receiving triplet therapy with chidaniline twice a week and dexamethasone once a week and venetoclax daily for 7 mo.

DISCUSSION

Primary PCL is one of the most aggressive leukemias.When compared to MM patients,more cases of pPCL have deletion of 1p,6q,13q,16q,and 17p and a significant gain of chromosome 1q than MM,showing poor prognosis[5].FISH analysis in our patient showed deletion of 13q14 and amplification of 1q21,suggesting a poor prognosis.First-line induction treatment for pPCL combines immuno-regulatory drugs[6](thalidomide and lenalidomide) and proteasome inhibitors (bortezomib and carfilzomib)[7]as well as anthracyclines or alkylating agents.Allogeneic stem cell transplantation[8]or autologous stem cell transplantation[9]further improved patient survival.However,several studies have reported that many pPCL patients die within a few months after diagnosis.Due to the exceptionally poor prognosis of patients with pPCL,novel combination therapies are urgently needed.

The frequency of t(11;14) in pPCL is higher than that of newly diagnosed MM.The translocation of t(11;14) is related to high expression of BCL-2[10].Venetoclax (ABT199)inhibited antiapoptotic BCL-2 protein,resulting in tumor cell apoptosis[11].Venetoclax achieved successful treatment of relapsed/refractory MM in phase I-III clinical trials[12-14].Thus,a venetoclax based regimen could be a standard approach for treating pPCL patients with translocation t(11;14) abnormality[15,16].Although venetoclax is particularly effective in patients with t(11;14),the drug was also shown to be effective in patients without such genetic changes[12].Venetoclax treatment has never been tested or reported in pPCL without t(11;14).Our patient had more than two prior therapy failures and acquired resistance to proteasome inhibitors and immunomodulators.Although CAR-T has a short remission,treatment with dacetuzumab and XPO1inhibitors (Selinexor) was shown to be ineffective,and triple therapy of venetoclaxchidamide-dexamethasone was started as independent therapy with t(11;14).According to recent discontinuation of BELLINI phase 3 trial (M14-031),venetoclax showed a higher proportion of deaths due to infection when compared with the control group[14].Therefore,the Food and Drug Administration has temporarily suspended the evaluation of venetoclax in clinical trials for MM research treatment.This is because our patient had pancytopenia before treatment,and venetoclax at a low dose (300 mg/d) was taken continuously.

Table 1 Evolution of therapy in our patient

HDACIs are one of the most promising therapeutic drugs used for the treatment of many types of cancers.In the pathogenesis of MM,the imbalance of histone acetylation plays a vital role[17].The overall remission rate of HDACI-based program in clinical trials of MM is 42%-61%[18],and a promising targeted therapy for MM treatment was shown to be HDACIs.Panobinostat is a non-selective HDACIs approved by the Food and Drug Administration in 2015.It can be used in combination with bortezomib and dexamethasone for the treatment of refractory/relapsed MM[19].Panobinostat’s anti-myeloma activity alters the gene expression through epigenetic modification and inhibition of protein metabolism.In the MM cell line,panobinostat and venetoclax are used in combination to enhance anti-myeloma activity.This synergistic effect might be attributed to the activation of intrinsic apoptosis and the inhibition of mammalian target of rapamycin signaling pathway[20].However,panobinostat is shown to cause many adverse events,especially diarrhea,nausea,fatigue,and hematological toxicity.To reduce the adverse events associated with pan-HDACI,selective HDACIs with higher efficacy and lower toxicity might act as promising drugs for the treatment of MM[21].Chidamide is a new type of benzamide HDACI that can selectively inhibit the activity of class I HDACIs.In 2014,chidamide has been approved for the treatment of relapsed/refractory peripheral T-cell lymphoma by the China Food and Drug Administration.Many studies have shown that chidamide has anti-tumor effects in a variety of hematological malignancies (such as lymphoma,myeloma,and leukemia)[22].Many studies have confirmed the antimyeloma effect of chidamide,and it mainly promotes the G0/G1 arrest and apoptosis of G0/G1 in a caspase-dependent manner in myeloma cells[23].We herein reported the results of a highly successful treatment for the diagnosis of pPCL 5 years ago.The patient received six prior lines of therapy,including all available treatment drugs(such as thalidomide,lenalidomide,ixazomib,daratumumab,XPO1 inhibitor,and CAR-T).We described a 62-year-old female patient who took oral chidamide 30 mg twice weekly in combination with venetoclax 300 mg daily and dexamethasone 20 mg weekly for the treatment of relapsed/refractory pPCL.The patient achieved VGPR in four courses of treatment.At present,the original maintenance treatment is still continued for 7 mo.For the first time in this field,the efficacy of chidamide in combination with venetoclax for the treatment of pPCL has been explored and tried to provide more options for treatment of this disease.We believe that the combination of venetoclax-chidamide-dexamethasone has the ability to salvage high risk,multirefractory in patients without t(11;14).

Figure 2 Flow-cytometry analysis.A: Plasma cells (PCs) in the peripheral blood at the time of diagnosis (here exemplarily shown CD38 and CD138); B: PCs in the peripheral blood before venetoclax use; C: PCs in the bone marrow after two cycles of venetoclax.

CONCLUSION

The combination treatment of venetoclax-chidamide-dexamethasone has achieved successful results for refractory/relapsed pPCL.This is also the first case report that described the use of BCL-2 inhibitors in combination with HDACIs in a patient with refractory/relapsed sPCL.This case report showed that the triplet was well tolerated,and even lower doses of venetoclax might lead to a deeper response (VGPR) for several months,as shown by bone marrow cytology,FCM,and immunosolid phase electrophoresis.Based on the successful results obtained,it is necessary to conduct further clinical studies to explore the combination of venetoclax and chidamide therapy for the treatment of pPCL.

Figure 3 Hematoxylin and eosin staining and immunohistostaining of bone marrow clot section (400 ×).H/E: Hematoxylin and eosin staining.

Figure 4 Fluorescence in situ hybridization analysis.A: RB1 (13q14) gene deletion in fluorescence in situ hybridization; B: CKS1B (1q21) gene amplification in fluorescence in situ hybridization.