Biliary stent combined with iodine-125 seed strand implantation in malignant obstructive jaundice

Hui-Wen Wang,Xiao-Jing Li,Shi-Jie Li,Jun-Rong Lu,Dong-Feng He,Department of Interventional,Harbin Medical University Cancer Hospital,Harbin 150081,Heilongjiang Province,China

Abstract BACKGROUND Malignant obstructive jaundice is mainly caused by cholangiocarcinoma.Only a few patients are indicated for surgical resection,and the 3-year survival rate is <50%.For patients who are not eligible for surgery,biliary stent placement can relieve biliary obstruction and improve liver function and quality of life.However,restenosis after biliary stents has a poor prognosis and is a clinical challenge.Biliary stent combined with iodine-125 (125I) seed implantation can prolong stent patency and improve survival.AIM To evaluate the safety and efficacy of biliary stent combined with 125I seed strand implantation in malignant obstructive jaundice.METHODS We enrolled 67 patients between January 2016 and June 2018 with malignant obstructive jaundice and randomized them into a biliary stent combined with 125I seed strand treatment (combined) group (n = 32) and biliary stent (control) group(n = 35).All patients underwent enhanced computed tomography and magnetic resonance imaging and were tested for biochemical and cancer markers.Twelve patients underwent pathological examination before surgery.All patients were followed up by telephone or clinical visit.Postoperative liver function improvement,postoperative complications,stent patency time,and survival time were compared between the two groups.Prognostic risk factors were evaluated.RESULTS Technical success was achieved in all patients in both groups.Postoperative liver function improved significantly in all patients (total bilirubin,direct bilirubin,alanine aminotransferase,and aspartate aminotransferase decreased significantly in all patients,the P values were less than 0.05).There was no significant difference in preoperative or postoperative indexes between the two groups for changes in total bilirubin (P = 0.147),direct bilirubin (P = 0.448),alanine aminotransferase (P = 0.120),and aspartate aminotransferase (P = 0.387) between the two groups.The median stent patency time of the combined group was 9.0 ±1.4 mo [95% confidence interval (CI):6.3-11.8 mo],which was significantly longer than the that of the control group (6.0 ± 0.3 mo,95%CI:5.5-6.5 mo,P = 0.000).The median survival time of the combined group was 11.0 ± 1.4 mo (95%CI:8.2-13.7 mo),which was significantly longer than that of the control group (7.0 ± 0.3 mo,95%CI:6.4-7.6 mo,P = 0.000).Location of obstruction and number of stents were independent risk factors affecting prognosis.CONCLUSION Biliary stent combined with 125I seed strand implantation is safe and effective in malignant obstructive jaundice and improves stent patency time and median survival time.

Key Words:Biliary stent;125I;Obstructive jaundice;Malignant tumor;Clinical research;Surgery

INTRODUCTION

Malignant obstructive jaundice is caused by cholangiocarcinoma or peribiliary malignant tumor invasion and compression;onset of symptoms is generally insidious with only 10%-20% of patients indicated for surgical resection.The 3-year survival rate is <50%[1,2].For patients who are not indicated for or unwilling to undergo surgery,biliary stent placement can relieve biliary obstruction,improve liver function and quality of life,and prolong survival[3-5].However,restenosis after a biliary stent may significantly worsen prognosis and remains a clinical challenge.The main causes of restenosis include tumor growth,cholestatic accumulation,and granulation tissue hyperplasia.In recent years,reports of biliary stents combined with iodine-125 (125I)seed implantation seemed to prolong stent patency time and improved patient survival[6-8].However,most attempts to relieve stenosis caused by tumors utilized a technique of placing the125I seed in the middle of the stent while ignoring both ends.Because of overgrowth of the tumor and fibrous granulation tissue hyperplasia caused by frictional stimulation between the two ends of the stent and the bile duct wall,both ends of the stent can be reobstructed[9,10].Therefore,measures to prevent reobstruction may provide a greater benefit to patients.In this study,we evaluated extending the125I seed strand across the entire length of the stent.

MATERIALS AND METHODS

Ethical permission

This study was approved by the Medical Ethics Review Committee of Harbin Medical University Cancer Hospital and registered for the Chinese clinical trial (Registration Number:ChiCTR2000030878).

Inclusion and exclusion criteria

This was a single center,randomized,controlled study.The inclusion criteria were as follows:(1) Age 18-80 years;(2) Eastern Cooperative Oncology Group score ≤ 2 points;(3) Obstructive jaundice caused by pathologically or clinically diagnosed malignant tumor,and patient not indicated or unwilling to undergo surgery;(4) No severe cardiovascular and cerebrovascular diseases;(5) No severe coagulation abnormalities;and (6) Willing to participate in this study and give signed informed consent.The exclusion criteria were as follows:(1) Suspicion of benign biliary stricture;and (2)Patients with reobstruction after biliary stenting or drainage.

Patient characteristics

Based on previous reports,the median survival time after traditional biliary stent implantation is 3-8 mo[6,11-13].We assumed median survival time of 3 mo in the control group and 8 mo in the combined group.The α value was 0.05 (unilateral test),the power was 0.9,and we needed 30 cases in each group for the sample size.

Between January 2016 and June 2018,67 patients with malignant obstructive jaundice were treated in our center.All patients underwent enhanced computed tomography and magnetic resonance imaging and were tested for biochemical and cancer markers.Twelve patients underwent pathological examination before surgery.All patients were diagnosed with malignant obstructive jaundice.Envelopes were randomly assigned to the simple stent (control) group and the biliary stent combined with125I seed strand treatment (combined) group according to a 1:1 ratio.There were 35 patients in the control group and 32 in the combined group (one patient in the combined group was transferred to the control group for economic reasons).There were 35 patients with pancreatic cancer (21 in the control group and 14 in the combined group);19 patients with gallbladder cancer and cholangiocarcinoma (8 in the control group and 11 in the combined group);and 13 patients with lymph node metastasis from digestive malignant tumors (6 in the control group and 7 in the combined group).The statistician was blinded to group assignment.The general clinical characteristics of the two groups of patients are shown in Table 1.The differences between the two groups were not significant.

Stent and 125I seed

125I seeds were produced by Beijing Science and Technology Biotechnology Co.Ltd.(Beijing,China).Dimensions:length = 4.5 mm,diameter = 0.8 mm,effective radiation radius of 15-20 mm,half-life of 59.43 d,energy of 27.4 keV for X rays and 35.5 keV for R rays.The stent was a biliary stent produced by Nanjing Minitron Co.Ltd.(Nanjing,China) with a diameter of 10 mm and length of 40-80 mm.

Procedures

Under the guidance of ultrasound and digital subtraction angiography,an 18G needle was used to puncture the intrahepatic dilated bile duct.A guide wire was introduced in place of the puncture needle core,and the puncture needle sheath was pulled out.An 8F long arterial sheath was introduced through the guide wire,and two guide wires were introduced through the long arterial sheath after withdrawing the core of the arterial sheath.After removing the arterial sheath,the stent conveyor was placed along one of the guide wires.A customized125I seed strand was placed along an 8F long arterial sheath that was introduced along the other guide wire (Figure 1A-F).The125I seeds were placed in a 4F plastic tubeviaa seed gun,and both ends were heated and then clamped shut (Figure 1G).The number of125I seeds was calculated by:N =L/4.5 + 2 (L = stent length).Only simple stents were placed in the control group.After successful stenting and125I seed strand implantation,an external drainage tube was placed through the guide wire.After 2 mo,imaging examination was performed to confirm that the stent was unobstructed before the drainage tube was removed.

Follow-up

All patients were followed up by telephone or clinical visit.Telephone follow-up was performed every 2 mo to document the patient’s status.If a patient had new jaundiceor a significant increase in total bilirubin,abdominal enhanced computed tomography or magnetic resonance imaging was recommended to determine stent obstruction.Follow-up continued until a patient died or the end of the study.The overall survival period was calculated from the time the stent was placed to when the patient died or the end of the study.The stent patency time was from stent placement to stent blockage or the end of the study.

Table 1 Comparison of baseline clinical characteristics between the two groups

Statistical analysis

The c2test was used to compare the numerical data.Continuous variables were expressed as mean ± standard deviation,and changes in the laboratory tests in the same group were evaluated by pairedttest.Comparison of changes in preoperative and postoperative indexes in both groups was performed by independentttest.Survival was calculated using the Kaplan-Meier method,and factors affecting prognosis were analyzed using a Cox regression model.P<0.05 indicated that the difference was significant.Statistical analysis was performed using SPSS version 17.0.

Figure 1 Guidance by ultrasound and digital subtraction angiography.

RESULTS

Technical success rate,liver function relief,and complications

Both groups of patients were successfully implanted with biliary stents or125I seed strand,and the technical success rate was 100%.Liver function was significantly improved in both groups 5 d after surgery (total bilirubin,direct bilirubin,alanine aminotransferase,and aspartate aminotransferase decreased significantly in all patients;thePvalues were less than 0.05).There was no significant difference in preoperative or postoperative indexes between the two groups for changes in total bilirubin (P= 0.147),direct bilirubin (P= 0.448),alanine aminotransferase (P= 0.120),and aspartate aminotransferase (P= 0.387) (Table 2).No serious complications occurred in either group.

Stent patency time

The mean and median stent patency times for the combined group were 9.5 ± 0.6 mo[95% confidence interval (CI):8.3-10.7 mo] and 9.0 ± 1.4 mo (95%CI:6.3-11.8 mo),respectively.For the control group,they were 5.8 ± 0.2 mo (95%CI:5.4-6.3 mo) and 6.0± 0.3 mo (95%CI:5.5-6.5 mo),respectively.There was a significant difference between the two groups (P= 0.000) (Figure 2A).By the end of the study,18 patients in the combined group had stent occlusion,of whom four had percutaneous transhepaticcholangial drainage,and 19 patients in the control group had stent occlusion,of whom seven had percutaneous transhepatic cholangial drainage.

Table 2 Comparison of preoperative and postoperative changes in the two groups of patients

Survival time

The median survival time for the combined group was 11.0 ± 1.4 mo (95%CI:8.3-13.8 mo).For the control group,it was 7.0 ± 0.3 mo (95%CI:6.4-7.6 mo).There was a significant difference between the groups (P= 0.000) (Figure 2B).

Risk factors affecting prognosis of survival

Univariate and multivariate analysis showed that the type of obstruction and the number of stents were independent risk factors that affected the prognosis for survival.Patients with low biliary obstruction and a single stent had a better prognosis(Pvalues were 0.015 and 0.035,respectively;hazard ratios were 2.060 and 3.489,respectively) (Table 3).

DISCUSSION

To treat unresectable malignant obstructive jaundice,the first task is to remove biliary blockage,improve liver function,and relieve symptoms to prepare for further radiotherapy,chemotherapy,or other treatments.Biliary stent placement has become the preferred treatment method[3-5,14].However,after stent placement due to tumor overgrowth,tissue-reactive hyperplasia,and biliary formation,stent restenosis significantly affects the survival of patients[15-18].Therefore,prevention of restenosis after stenting has been a hot topic for clinical research and investigation in recent years and has included the study of covered and drug-eluting stents.Covered stents are prone to displacement,and stent displacement tends to increase the incidence of cholecystitis and pancreatitis.These two approaches have not achieved satisfactory clinical outcomes[19-21].

125I seeds are new micro-low-dose-rate particle sources developed in recent years with an effective irradiation radius of 15-20 mm,half-life of 59.43 d,and energy of 27.4 keV for X rays and 35.5keV for R rays.Low dose rate and continuous irradiation lead to high dose within the target,resulting in a strong targeted killing effect on tumorcells by destroying DNA double strands of tumor cells and reducing their ability to proliferate without significantly affecting surrounding normal tissues.Because of these advantages,125I seed implantation has achieved good results in the treatment of various solid malignancies in recent years.

Table 3 Univariate and multivariate analysis of overall survival

Chenet al[22]first implanted125I seeds into porcine bile duct.They demonstrated that125I seeds were safe and did not cause serious damage to the bile duct.There are also reports that biliary stents combined with125I seed strands for patients with malignant obstructive jaundice.Due to the killing effect of125I seeds on tumor tissues,they can increase stent patency time and prolong survival[6,8,11,12,23].Some researchers placed the125I seed strands into biliary stenosis through an external drainage tube and then removed the drainage tube and125I seed strands 2 mo later.This approach also improved the stent patency time and survival of patients compared with traditional stents[6,8,11,12,23],but the drainage tube was unstable due to respiratory movement and other daily activities and affected quality of life.Because the drainage tube and125I seed strands were removed after 2 mo,the125I seed strands had a shorter duration of action,which inevitably affected treatment efficacy.

Subsequently,Zhuet al[6]developed a biliary stent carrying125I seeds and placed the biliary stent into 12 patients with malignant obstructive jaundice.They showed that radioactive particle stents were superior to traditional bare stents.However,because the stent was divided into a jacket and an inner stent carrying particles,the inner diameter of the stent for drainage was reduced,and the narrowing of the stent inevitably affected bile drainage and stent patency time[15,24].Additionally,other researchers inserted the125I seed strands between the stent and bile duct wall,and the stent patency time and patient survival also improved[25].The125I seed strands are fixed and stable,and the quality of life can be significantly improved without carrying an external drainage tube for a long time.We also used this approach with a satisfactory outcome and quality of life was significantly improved.However,all of the aforementioned studies focused on the stenosis caused by existing tumors and placed125I seeds in the middle of the stent,ignoring both ends of the stent.

Tumor growth will occur along the outside of the stent,causing obstruction at both ends of the stent as reported previously[9].Guoet al[26,27]found that after esophageal stent implantation,both ends were prone to reocclusion in animal experiments and clinical practice.Pathological examination revealed that the obstruction was mainly caused by overgrown tumor tissue and hyperplastic granulation tissue.After biliary stenting,granulation tissue hyperplasia may also occur with local frictional stimulation between both ends of the stent and the biliary tract[10].These factors can cause reobstruction at both ends of the stent.125I seeds destroy double-stranded DNA of tumor cells and stop tumor proliferation.Additionally,it has been found that radioactive stents increase the activity of the caspase-3 gene through γ rays,promoting canine bile duct smooth muscle cell apoptosis,and therefore inhibiting canine bile duct stenosis in animal experiments[28].

Figure 2 Median stent patency times and survival time for the combined group.

Based on the inhibitory effect of125I seeds on tumor and granulation tissues,we extended the coverage of125I seed strands to both ends of the biliary stent to prevent reobstruction at either end.Our results showed that the survival time of the combined group reached 11.0 ± 1.4 mo,which is longer than that from the Zhuet al[6]study(median survival time 7.4 mo).The stent patency time reached 9.0 ± 1.4 mo,which was significantly better than that reported by Zhuet al[6](stent patency time and median survival time of 194 d)[13].A few factors may have contributed to the improved outcomes in our study.Firstly,the extended125I seed strand played a role in preventing obstruction at both ends of the stent.Secondly,the diameter of our stent was 10 mm.Some studies have shown that the larger the diameter of the stent,the longer the restenosis time appears to be[15].Thirdly,if there was biliary tract infection,we performed conventional percutaneous transhepatic cholangial drainage before biliary stent placement to reduce biliary edema and control infection.When the biliary stent was implanted,the catheter was first used to drain the accumulated viscous bile,and normal saline was used to wash out the remaining bile.

The stent was placed after bile drainage,which seemed to prolong stent patency time,presumably because thick bile can rapidly cause reobstruction of the stent[15,24].Our study also found that the type of obstruction and number of stents were independent risk factors that affected prognosis.The higher the location of the obstruction,the more and thinner the bile duct branches would be.Because left and right hepatic ducts are disconnected,bilateral stents are required.One stent can only resolve one obstruction.If there are more than two biliary obstructions,then the stents cannot resolve all the obstructions.Therefore,multibranch biliary obstruction has a generally poor bilirubin declining effect,which directly affects liver function and prognosis.

There were some limitations to our study.First,the sample size was small,and a multicenter study with a large sample size is needed.Second,due to the lack of advanced measuring instruments,accurate metrological data could not be obtained.

CONCLUSION

In conclusion,biliary stent combined with125I seed strand implantation is safe and effective for treatment of malignant obstructive jaundice.The present study suggests that it could improve stent patency and median survival.

ARTICLE HIGHLIGHTS

Research background

Biliary stent placement can relieve biliary obstruction and improve liver function and quality of life.However,restenosis after biliary stents has a poor prognosis and is a clinical challenge.

Research motivation

In order to solve the problem of biliary stent restenosis,we should seek a better method for clinical treatment.

Research objectives

To evaluate the safety and efficacy of biliary stent combined with iodine-125 seed strand implantation in malignant obstructive jaundice.

Research methods

Sixty-seven patients were randomly divided into the biliary stent with iodine-125 group (combined group) and the biliary stent group (control group).The stent patency time and total survival time were compared between the two groups.

Research results

The present study suggests that biliary stent combined with iodine-125 seed strands could improve stent patency and median survival.

Research conclusions

Biliary stent combined with125I seed strand implantation is safe and effective for the treatment of malignant obstructive jaundice.

Research perspectives

A multicenter study with a large sample size is needed.Accurate metrological data is needed.