MicroRNA146a與CXCR4ぴ諤悄蠆⌒募〔⌒募〕上宋細胞中的表達變化

王璨 陶輝 蔣書美 王立超 石開虎

摘?要：目的：探究微小RNA146a（miR146a）與趨化因子受體4（CXCR4）在糖尿病心肌病心肌成纖維細胞（CFs）中的表達變化。方法：提取原代心肌成纖維細胞（Cardiac?fibroblasts，CFs），高糖（33.3mmol·L1）作用CFs。用qRTPCR檢測CFs中miR146a的表達，Western?blotting檢測CFs中α平滑肌肌動蛋白（αSMA）、Ⅰ型膠原前膠原A1（Col1A1）、CXCR4蛋白的表達情況。MTT檢測高糖對CFs增殖活性的影響。結果：與正常對照組相比，高糖誘導后的CFs中miR146a表達量降低，而高糖誘導后的CFs中αSMA、Col1A1、CXCR4表達增加。并且高糖處理后CFs增殖活性提高。結論：糖尿病心肌病形成過程中CFs中miR146a表達下調，而CXCR4表達上調，提示miR146a與CXCR4糖尿病心肌病的病程中可能存在潛在的調節關系，有助于探索糖尿病心肌病的病理機制。

關鍵詞：miR146a;CXCR4;糖尿病心肌病;心肌纖維化

中圖分類號：R?542.23

To?investigate?the?expression?changes?ofMicroRNA146a?and?CXCR4

in?myocardial?tissue?and?fibroblasts?of?diabetic?cardiomyopathy

Wang?Can1，2?Tao?Hui1，2?Jiang?Shumei1，2?Wang?Lichao1，2?Shi?Kaihu1，2*

1.Dept?of?Cardiothoracic?Surgery，The?Second?Affiliated?Hospital?of?Anhui?Medical?University?AnhuiHefei?230601;

2.Cardiovascular?Research?Center，Anhui?Medical?University?AnhuiHefei?230601

Abstract：Objective：To?investigate?the?expression?changes?of?microRNA146a?（miR146a）?and?chemokine?receptor?4?（CXCR4）?in?diabetic?cardiomyopathy?myocardial?fibroblasts?（CFS）.Methods：CFS?were?extracted?and?high?glucose?（33.3mmol·L1）?was?used?to?induce?CFs.Use?qRTPCR?in?the?detection?of?CFs?miR146a?express，Western?blotting?detection?in?CFs?the?expression?of?αSMA，Col1A1，and?CXCR4.MTT?was?used?to?detect?the?effect?of?high?glucose?on?the?proliferation?of?CFS.Results：Compared?with?the?normal?control?group，the?expression?of?miR146a?decreased?in?CFs?induced?by?high?glucose，while?the?expression?of?αSMA，Col1A1?and?CXCR4?increased?in?CFs?induced?by?high?glucose.The?proliferation?activity?of?CFs?was?increased?after?high?glucose?treatment.Conclusion：During?the?formation?of?diabetic?cardiomyopathy，miR146a?expression?is?downregulated?in?CFs，while?CXCR4?expression?is?upregulated，suggesting?that?there?may?be?a?potential?regulatory?relationship?between?miR146a?and?CXCR4?in?the?course?of?diabetic?cardiomyopathy，which?is?helpful?to?explore?the?pathological?mechanism?of?diabetic?cardiomyopathy.