Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Plant drought Tolerance

Leaf Morphology GenesandCo-Regulate Cellulose Synthesis and Affect Plant drought Tolerance

Fig. S1.affects yield traits of rice.

A–C, Morphology of panicle (A, bar is 5 cm), grain width (B, bar is 1 cm), and grain length (C, bar is 1 cm) of rice grains;

D–K, Comparison of panicle length (D), the number of branches at a time (E), the number of secondary branches (F), grain number per panicle (G), grain length (H), grain width (I), seed-setting rate (J), and 1000-grain weight (K). Data are Mean ± SD/SE (= 3), and the lowercase letters above the bars represent the significant difference at the 0.05 level.

Fig. S2. Soil drought tolerance of mutants.

A, Soil drought tolerance (Bar = 10 cm). B, Partial enlarged view. C, Survival rate. Data are Mean ± SD/SE (= 3), and the lowercase letters above the bars represent the significant difference at the 0.05 level.

Table S1. Oligonucleotides used in this study.

MarkerSense primer Antisense primer B-14CCTCTTAAACACACCCGAACAATGGTTGAGATTCGGAGA B-15ATCAGGATCTATGAACTTGCAATCAGCTACACTTTGGACTCA B-16TCACCGTCACCTCTTAAGTCGGTGGTTGTGTTCTGTTTGG C-1AAAGACGACCAATAGCATATCGAAATTGAAACGGAGTA C-2CCGCAAGTCATGTCCTAAATGCCATAGTAGCCATCCA C-3GGTGATGCCGTAGACGTTGGTTGGGTTGAATGGGCTTGG C-5AAGTCGGCATTGTTTGAGATCCAATGGACGAGGGGTTG C-7CATTATTTCTTATCGTTGGCCATCATTTGAAAAGTAGC C-8TCCTCCACTGTGAACTCTATACCGGACTGAAACTAAAA OsCSLD1-RTATCTCTACCCCTTCGCCAAGCCAGAGCAAGGAGATGGTGA OsCSLD2-RTGGTTAACCTGATCGCCATCGTCCTTCCCATTAGCCCCTTG OsCSLD3-RTGAGGACCATCTACAGCGACACAGGGAGACGGTGATTGAGA OsCSLD5-RTGCACTACTACCCGTTCGCCGAGGAGAGAGACGGTGATGGAG OsCESA1-RTCTCAAGGGTCTCATGGGCACGATCTTGACCCACAGAAGG OsCESA2-RTTATTTGCGGTCTTCCAGGGTTGGGATCAGAAGGGTTGTCC OsCESA3-RTTTCATTGGGGTTGTTGCTGGGGACGATCACCCAAAATGCA OsCESA4-RTGAGCTGTACGTGTTCAAGTGGAAGAACACCTTGCCGAAGAGC OsCESA5-RTTTGGGTCATTGGAGGTGTCTTAGGAGCAAAGTGGTAGGGG OsCESA7-RTCGACGACGCTGATCATCATCAAGGGGTAGAGGTGGAGGAT OsLAC17-RTATCCGTTTCCTCGCTGACAACATCCAACACTAGCCATGCC OsDRP2B-RTCAAGAGGACCACAATGCTAAACGGGTATACGATGCAACAGAAGCTC OsRCI2-5-RTCGTCTTCCTGCGCTACGGCCAGCACATACACCGCGTA OsGSK1-RTAGAATGCCTCCAGAAGCCATCGCTGGCTAGTTCATGCTTG OsSKIPa-RTGGCGATGCAGATGAACAACTTCTCAACAGCTTTCTTCCCCT OsNAC6-RTAGAGCCAGCCCAAGATCAGCTTGCCCCAGTACATGAGGA SRL1-RTCCACTAAGCTTTCCAGACACGCTGTCCATAGCCTTGCAACA RENL1-RTAAGAGGGACTTCCTCAAGAACAAGTCGTACTCGCGCTTCACCTT RENL1-Cas9ggcaCGACGGCGCGCAACCCCAAaaacTTGGGGTTGCGCGCCGTCG RENL1-OVERttcgagctcggtaccATGTCGCGGCGGCTGTCGTTgactctagaggatccCTATGGGAAGCTGAATCCGC