Effects of low dose Glibenclamide on secondary damage after acute spinal cord injury in rats

李熙(Li Xi，Dept Spinal Surg，1st Affil Hosp Fujian Med Univ，F(xiàn)uzhou 350005)…∥Chin J Orthop.－ 2010，30(11).－1151～1156

Effects of low dose Glibenclamide on secondary damage after acute spinal cord injury in rats

李熙(Li Xi，Dept Spinal Surg，1st Affil Hosp Fujian Med Univ，F(xiàn)uzhou 350005)…∥Chin J Orthop.－ 2010，30(11).－1151～1156

ObjectiveTo investigate the effects of Glibenclamide on reduction of secondary damage after acute spinal cord injury in rats.MethodsNinety rats were randomly divided into control group(laminectomy alone)，spinal cord injury group(injury group)，and treatment group(treated with Glibenclamide after spinal cord injury)，with 30 rats in each group.The pathological morphology changes of injured spinal cord were observed by HE staining and electron microscope.The expressions of sulfonylurea receptor 1(SUR1)were detected by immunohistochemical method at 45 min，6 h，24 h，3 d and 7 d after spinal cord injury，and IPP 6.0 software were used for quantitative analysis.The function recoveries of the hind limbs of rats were evaluated by BBB score.The blood sugar level was detected quantitatively.ResultsHE staining showed that tissue bleeding and microglia proliferation getting severe with time after spinal cord injury.Compared to the injury group，tissue bleeding，microglia proliferation and inflammatory cell invasion was less severe in treatment group.Showed by electron microscope，inflammatory cell invasion，myelin sheath layer structure damage and mitochondrial swelling were significantly reduced after Glibenclamide treatment.Detected by immunohistochemical staining，the expressions of SUR1 at all time points after injury，except for 45 min that there were no SUR1 expressions in all groups，were much weaker in the treatment group than in the injury group.The SUR1 expression reached the peak at 24 h after injury in injury group，and decreased gradually with time.Significant differences were found in the SUR1 expression among three groups by oneway ANOVE.The BBB scores of treatment group were significant higher than that of injury group.The blood sugar slightly decreased in the treatment group，while nosignificant difference was found among three groups.ConclusionGlibenclamide can significant reduced the secondary damage after acute spinal cord injury.The protection of Glibenclamide after spinal cord injury may relate to its suppression of SUR1.7 refs，4 figs，2 tabs.

(Authors)