Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro

王 哲(Wang Zhe，Dept Spine Surg，Xijing Hosp，4th Mil Med Univ，Xi’an 710032)…∥Chin J Orthop.－2010，30(11).－1138～1143

Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro

王 哲(Wang Zhe，Dept Spine Surg，Xijing Hosp，4th Mil Med Univ，Xi’an 710032)…∥Chin J Orthop.－2010，30(11).－1138～1143

ObjectiveTo investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.MethodsThe human ligament cells were divided into three groups according to its sources，including normal yellow ligament cells(NLF)group(from acute traumatic thoracolumbar fractures with paraplegia in 7 patients)，degenerative yellow ligament cells(DLF)group(from degenerative lumbar stenosis in 9 cases)and ossified ligament cells(OLF)group(thoracic yellow ligament from 8 patients).Twenty-four groups of cells were obtained under vitro cell culture by the method of tissue adherence.Different concentrations of NaF were added into the medium when the cells spread to the fifth generation.Then，the morphological changes were observed and ALP activity and BGP synthesis were tested.ResultsHuman yellow ligament cells from different samples can proliferate and be passaged in vitro.The cell in ossific groups and degenerative groups were pleomorphic and could form calcium nodules.High concentration of NaF(1.0 mmol/L)can lead to cytotoxic reaction in all 24 groups.Low concentrations of(0.01 － 0.125 mmol/L)NaF can enhance the ALP activity and BGP synthesis in DLF groups while no effect was found in OLF and NLF groups cells under the same concentration of NaF.ConclusionThe fact that fluoride can promote ALP activity and BGP synthesis in degenerative yellow ligament cells in vitro indicates that fluoride may play an important role in inducing further ossification of human ligament cells.16 refs，12 figs.

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