Establishment of rat experiment model of chronic allograft nephropathy

Yin Lipig(殷立平， Jiangsu Provin Hosp Tradit Chin Med Nanjing Univ Tradit Chin Med，Nanjing 210029)…∥Chin J Transplant.－2011，32(7).－433～437

Establishment of rat experiment model of chronic allograft nephropathy

Yin Lipig(殷立平， Jiangsu Provin Hosp Tradit Chin Med Nanjing Univ Tradit Chin Med，Nanjing 210029)…∥Chin J Transplant.－2011，32(7).－433～437

ObjectiveTo summarize experience of establishing stable rat model ot chronic allograft nephropathy.MethodsWe used Fisher rats as donors and Lewis rats as recipients.After left kidney of donor perfused in situ under hypothermic condition，left renal vein，abdominal aorta and bladder flap of donor was anastomosed with left renal vein，renal artery and bladder of the recipient，respectively.The recipients were given cyclosporin oral solution 10 mg/kg every day by gavage for 10 days after transplantation.The blood and urine samples were collected 1 month，2 months and 4 months after transplantation and renal function and total urine protein were examined.The pathological changes of renal allograft were observed 2 and 4 months after transplantation.Results

(Authors)

Forty－five rats

operation and achievement ratio was 85%.The renal transplantations finished in 120±20 min after living related kidney transplantation in individualized immuuossuppressive therapy Scr，BUN，Cycs and total urine protein demonstrated a significant increase one month after transplantation.At the second and fourth months，with exception of urine protein continued to increase，the other indicators did not change significantly.Two months after transplantation renal pathology demonstrated light to moderate interstitial fibrosis，infiltration of lymphocytes and plasma cells.At 4th month the renal allografts showed extensive interstitial fibrosis，a large number of infiltrating interstitial cells，thickening，hardening，occlusion of glomerular basement membrane，and renal tubular atrophy that were consistent with pathological changes of chronic allograft nephropathy.ConclusionThrough adequate surgical training and improvement，and specification for rat nephrectomy，transplantation surgery，and postoperative management in every detail，the model with high success rate and stability can be achieved.10 refs，4 figs，4 tabs