叢枝菌根真菌對黃檗耐鹽能力的影響1)

趙匠 徐佳晶 李霞

(吉林師范大學，四平，136000)

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叢枝菌根真菌對黃檗耐鹽能力的影響1)

趙匠 徐佳晶 李霞

(吉林師范大學，四平，136000)

采用溫室盆栽試驗，研究鹽脅迫下，接種摩西球囊霉(Glomusmosseaw)和根內球囊霉(Glomusuntraradices)對黃檗耐鹽能力的影響。結果表明：鹽脅迫下，叢枝菌根(AM)真菌對黃檗侵染率影響不顯著(p>0.05)，黃檗葉片丙二醛(MDA)質量分數顯著高于對照處理，接種AM真菌處理的黃檗葉片MDA質量摩爾濃度均顯著低于不接種處理(p<0.05)；脯氨酸質量分數均較未接種植株升高，AM真菌有效地減輕黃檗受脅迫的程度。接種AM真菌顯著增加了可溶性蛋白質量分數，AM真菌增強了黃檗非酶促防御系統能力。鹽脅迫下，過氧化物酶(CAT)和過氧化氫酶(POD)活性在菌根和非菌根處理間差異不顯著(p>0.05)，超氧岐化酶(SOD)活性在菌根和非菌根植株處理間差異顯著(p<0.05)。接種AM真菌后，菌根化黃檗植株可通過滲透調節物質積累和抗氧化酶活性的提高，降低體內膜脂過氧化產物的水平，緩解脅迫對黃檗植株的傷害，增強了黃檗對鹽脅迫的耐受力。

叢枝菌根真菌；黃檗；鹽脅迫

The experiment was conducted to study the effect of inoculated withGlomusmosseawandG.untraradiceson salt tolerance ability under salt stress by a pot experiment under greenhouse conditions. The effect of infection rate ofArbuscularmycorrhizal(AM) fungi under salt stress onPhellodendronamurensehas no significance (p>0.05). MDA content in leaves ofP.amurenseunder salt stress was significantly higher than that of the control treatment, and the MDA content in leaves ofP.amurenseinoculated with two species fungi treatment was significantly lower than that of non-inoculation treatment (p<0.05). The proline content was higher than that of non inoculation treatment, AM fungi can effectively reduce the degree of stress ofP.amurense. Inoculated treatment with two species fungi significantly enhanced soluble protein, and AM fungi can enhance non-enzymatic defensive System ability ofP.amurense. There were not significantly difference mycorrhizal and non-mycorrhizal treatments of CAT and POD activities under salt stress (p>0.05), in addition, there was significantly different between the mycorrhizal and non-mycorrhizal plants of the SOD activity (p<0.05). After inoculated with AM fungi, mycorrhizal plants ofP.amurensecan be, through osmoregulation substance accumulation and antioxidant enzyme activity, raised to reduce the level of lipid peroxidation productions, to relieve the stress on the plant damage ofP.amurense, and to enhance the salt tolerance ofP.amurense.

叢枝菌根(Arbuscularmycorrhizal，AM)真菌能與80%的植物建立共生關系，加強宿主植物對土壤養分和水分吸收，促進植物生長。通過提高抗逆相關酶活性，減緩逆境脅迫影響，對植物的抗逆境脅迫能力具有一定的影響[1-3]。土壤鹽害是限制植物生長發育的重要因素，全世界有超過7%的鹽堿土地，而在干旱和半干旱地區這一比例可達15%[4]。研究發現，逆境脅迫下，AM真菌增加葉片可溶性蛋白、脯氨酸質量分數[5-6]，提高宿主植物質膜ATPase和液泡膜ATPase的活性[3]；調節細胞對K+和Na+的運輸與吸收，并通過提高抗逆相關酶活性及植株N和P質量分數，調節植物滲透勢，保持細胞內外離子平衡，以減緩逆境脅迫對宿主植物的影響[7]。國內外有關AM真菌與植物的互惠共生關系的研究，發現AM真菌在提高植物抗逆境脅迫能力(如耐旱、耐鹽和鹽堿地改良)有著非常重要的作用[8-11]。

黃檗(PhellodendronamurenseRupr.)是蕓香科黃檗屬植物，主要分布在黑龍江、吉林、遼寧等地區。黃檗是重要的用材樹種，也是珍貴的中藥材，具有重要的經濟價值。關于……