側(cè)腦室內(nèi)注射神經(jīng)肽S對瑞芬太尼所致痛覺過敏小鼠鎮(zhèn)痛效果的研究

丁晨 馬婷婷 胡兵偉

[摘要] 目的 研究側(cè)腦室內(nèi)注射神經(jīng)肽S對瑞芬太尼痛覺過敏小鼠鎮(zhèn)痛效果的影響。 方法 將雌性SPF級BALB/c小鼠隨機分為對照組、切口痛組、瑞芬太尼組和切口痛-瑞芬太尼組。待切口痛-瑞芬太尼組小鼠造模成功后側(cè)腦室注射給藥，分為NS（生理鹽水）組和2 nmol/L NPS組（神經(jīng)肽S）、4 nmol/L NPS組、6 nmol/L NPS組、8 nmol/L NPS組。通過側(cè)腦室注射不同劑量NPS及NS，檢測小鼠機械痛縮足閾值和熱輻射縮足潛伏期。 結(jié)果與同組基礎(chǔ)值相比，術(shù)后切口痛組、瑞芬太尼組和切口痛-瑞芬太尼組的機械痛縮足閾值和熱輻射縮足潛伏期明顯減少;術(shù)后6 h、24 h、48 h與對照組相比，切口痛組、瑞芬太尼組和切口痛-瑞芬太尼組的機械痛縮足閾值和熱輻射縮足潛伏期明顯減少;成功構(gòu)建切口痛-瑞芬太尼組痛覺過敏小鼠模型并側(cè)腦室注射給藥后，與NS組相比較，NPS組給藥后的小鼠機械痛縮足閾值和熱輻射縮足潛伏期明顯增加;與給藥前相比，NPS各濃度組的小鼠機械痛縮足閾值和熱輻射縮足潛伏期在給藥后30 min內(nèi)隨著時間的延長而增加。 結(jié)論 在切口痛-瑞芬太尼痛覺過敏小鼠疼痛模型中，側(cè)腦室注射NPS對瑞芬太尼引起的痛覺過敏有明顯的鎮(zhèn)痛效果。

[關(guān)鍵詞] 神經(jīng)肽S;瑞芬太尼;鎮(zhèn)痛效果;痛覺過敏

[中圖分類號] R614 ? ? ? ? ?[文獻標(biāo)識碼] A ? ? ? ? ?[文章編號] 1673-9701（2020）12-0037-05

[Abstract] Objective To study the analgesic effect of intraventricular injection of neuropeptide S on the analgesic effect of remifentanil-induced hyperalgesia in mice. Methods Female SPF BALB/c mice were randomly divided into control group， incision pain group， remifentanil group and incision pain-remifentanil group. After the incisional pain-remifentanil-induced hyperalgesia mice were successfully modelled， the drugs were injected into lateral ventricle and the mice were divided into the NS （normal saline） group and the 2 nmol/L NPS group （neuropeptide S）， the 4 nmol/L NPS group， 6 nmol/L NPS group， 8 nmol/L NPS group. By injecting different doses of NPS and NS into the lateral ventricle， the threshold of mechanical pain withdrawal and the latency of thermal radiation withdrawal were measured. Results Compared with the basic values in the same group， the postoperative incision pain group， the remifentanil group and the incision pain-remifentanil group had significantly reduced mechanical foot withdrawal thresholds and thermal radiation foot withdrawal latency. Compared with the control group at 6 h， 24 h and 48 h after operation， the incision pain group， remifentanil group， and incision pain-remifentanil group had significantly reduced mechanical foot withdrawal thresholds and thermal radiation foot withdrawal latency. After the model of hyperalgesia in the incision pain remifentanil group was successfully constructed and injected into the lateral ventricle， compared with NS group， the mechanical pain foot retraction threshold and thermal radiation foot retraction latency in NPS group increased significantly. Compared with those before administration， the mechanical pain withdrawal threshold and thermal radiation withdrawal latency of mice in each concentration group of NPS increased with time within 30 min after administration. Conclusion In mice model of incisional pain-remifentanil-induced hyperalgesia， lateral ventricle injection of NPS has a significant analgesic effect on remifentanil-induced hyperalgesia.