Diagnosis of cytomegalovirus encephalitis using metagenomic nextgeneration sequencing of blood and cerebrospinal fluid: A case report

lNTRODUCTlON

Cytomegalovirus (CMV) is a type of herpesvirus[1]. Its gene structure is very similar to herpes simplex virus. Humans are the only host of human CMV, and virus carriers are the most common source of infection[2]. CMV infections are mostly subclinical, inapparent, and latent[1,2]. When the body’s immune function is compromised, CMV can infect multiple organs and systems, manifesting clinical symptoms[3]. The infections of CMV are most common in the lung, followed by liver, kidney, but brain tissues are rarely affected[4,5]. This is different from herpes simplex virus, which is the most common cause of encephalitis. Most of the reported cases of CMV encephalitis had immunodeficiency, such as HIV infection or organ transplantation[6,7]. Studies on CMV patients with normal immune function were few. Its diagnosis and detection rate need to be further improved.

In fact, the more he considered the matter, the more he felt that he would be wise to put a good face on it, and to let people suppose that he had really brought about the marriage of his own free will

The diagnosis of CMV encephalitis is mainly based on the results of pathogenic examinations. There are no well-defined diagnostic criteria currently. Methods including serology, culture, quantitative PCR (qPCR), antigenemia, histopathology, and CMV-specific T-cell detection are used for the diagnosis, the monitoring of priority treatment, and the antiviral response[8]. However, there are some limitations. For example, the sensitivity of serology and culture is always low, and antigenemia needs enough leukocytes in the samples.

He emptied the purse altogether and then shut it; when he opened it the fifty crowns were still there!Then a plan came into his head, and he determined to go at once to the Court tailor and coachbuilder

Metagenomic next-generation sequencing (mNGS) has been recently applied more often in pathogen examination of infectious diseases[9]. It can accurately analyze all microorganisms in samples, with results being obtained within 24 h. It could be used as a rapid screening tool for patients with suspected CMV encephalitis.

Abnormal electroencephalogram (EEG) was recorded on November 9. Starting from the right hemisphere leads, the amplitude gradually increased and the frequency decreased after appearance of a fast-wave rhythm of 14.0-22.0 Hz, 15-50 μV. Slow-wave rhythm was observed on all leads, and sharpwave rhythm was observed in the right occipital area, lasting 30-60 s, without obvious clinical seizure or discomfort during this time.

Here we report a patient who was finally diagnosed with CMV encephalitis with the help of mNGS of the blood and cerebrospinal fluid (CSF) samples. This was confirmed by qPCR of blood samples.

CASE PRESENTATlON

Chief complaints

In the early morning of November 8, 2020, the patient suddenly screamed with involuntary tremor of the left upper limb during sleep, out of breath, followed by disturbance of consciousness with eyes tightly closed. The conditions lasted for about half an hour prior to relief. Then, the patient felt dizziness and weakness of the four limbs accompanied by slurred speech. The symptoms were completely relieved in 3-4 h.

History of present illness

An 86-year-old male was admitted on November 8, 2020 due to sudden onset of unconsciousness for 3 h.

History of past illness

The patient had a history of hypertension and diabetes for more than 10 years. Antihypertensive drugs were taken regularly, but no hypoglycemic agent was administered.

Personal and family history

In this case, the patient’s initial manifestations were epileptic seizures and status epilepticus, accompanied by the signs of left side paresis and unsteady gait. Despite the clear clinical manifestations and imaging evidence of brain parenchymal impairment, the diagnosis of this disease was difficult. Tests including initial CSF routine, biochemistry, ink staining, acid-fast staining smears, cryptococcus capsular antigen detection, and IgG were carried out, all of which only temporarily reduced the possibility of tuberculosis and fungal and bacterial infections. The possibility of special infection was not ruled out. Their values in guiding our clinical treatment were limited. For patients with acute and critical illness of the central nervous system, time is crucial.

The cook was terrified when he heard the command, and said to the Many-furred Creature, You must have let a hair fall into the soup, and if you have you deserve a good beating! When he came before the King, the King asked who had cooked the soup

Physical examination

The blood pressure and glycemic control were basically normal.

Laboratory examinations

On November 12, a lumbar puncture was performed with the pressure at 250 H

O. CSF routine revealed the nucleated cell counts at 4 × 10

/L, chloride at 124.5 mmol/L, CSF pressure at 0.58 ↑g/L, glucose at 3.56 mmol/L, anti-SS-A antibody (+), ANA cytoplasmic granular pattern, ANA titer at 1:100, anti-TPO at 198.8 U/mL, and anti-TG at 128.8 U/mL. Anti-sulfatide antibody IgG was positive. The timeline information of this case was shown in Figure 1.

Imaging examinations

On the third day the messenger returned and announced: I have not been able to find any new names, but as I came upon a high hill round the corner of the wood, where the foxes and hares bid each other good night, I saw a little house, and in front of the house burned a fire, and round the fire sprang the most grotesque little man, hopping on one leg and crying:

One day when she had grown up, her father looked at her, and saw that she was exactly like her mother, so he said to his councillors, I will marry my daughter to one of you, and she shall be queen, for she is exactly like her dead mother, and when I die her husband shall be king

Brain magnetic resonance imaging and magnetic resonance angiography were consistent with subcortical arteriosclerotic encephalopathy and indicated ischemic changes in the right postcentral gyrus and possible early laminar cortical necrosis (Figure 2). Magnetic resonance angiography showed cerebral arteriosclerosis manifestations and reduced branching of the left middle cerebral artery, suggesting poor blood supply. On November 11, video-EEG during epileptic seizures showed lowamplitude fast-wave rhythms at 15.0-22.0 Hz, on all leads, with increasing amplitude and gradually decreasing frequency (Figure 2). After about 30 s, slow-wave activities were observed on all leads, and mixed with a large number of sharp waves and sharp-slow waves, mainly on the right side. Twitching of the right upper limb occurred at this point and recurred at a 4-12 min intervals. This seizure pattern recurred many times, during which the EEG indicated that the symmetrical appearance of 14.0-22.0 Hz, 5-15 μV β-rhythm was the basic rhythm, emitting a large number of 5.5-7.5 Hz low-amplitude θ waves (Figure 2).

FlNAL DlAGNOSlS

Blood and CSF samples were submitted for PACEseq mNGS detection (Hugobiotech, Beijing, China) on December 16, 2020. CMV was found in CSF samples of 1 unique read and in blood samples of 614 unique reads (Figure 3A and B). Simultaneous qPCR of blood samples confirmed the mNGS results, while it failed to detect CMV in CSF samples (Figure 3C). CMV meningitis with status epilepticus was finally diagnosed in this patient.

TREATMENT

CMV infection is very common among people. Cellular immunity plays an important role in the defense of infections; when cellular immunity decreases, the incidence of infection and the onset of disease will increase. Some studies showed CMV encephalitis increased at the beginning of organ transplantation and cancer chemotherapy[10]. CMV infections in patients with normal immune function are few.

So the girl seated herself on the horse, and rode for a long, long way, and at last she came to the mountain, where an aged woman was sitting outside with a gold carding- comb

OUTCOME AND FOLLOW-UP

After discharge, the patient regularly took antiviral, low-dose hormone, anti-epileptic, and other medications. He was admitted to the Third Affiliated Hospital of Sun Yat-sen University for the second time on January 8, 2021. Physical examination, paralysis test of the left upper limb, and Romberg’s test showed the same results as before. After admission, the patient completed relevant examinations. Antinuclear antibody test of the blood on January 13, 2021 was weakly positive with 1:80 granular pattern, showing anti-SmD1 antibody 118 AU/mL, anti-SS-A antibody 227 AU/mL, and anti-SS-B antibody 202 AU/mL. Salivary gland tissue was submitted for examination. The lobular structure was preserved, with some acinar atrophy. Hyperplasia of interlobular adipose tissue was observed. There were obvious lymphocyte and plasma cell infiltration in the interstitium, and one lymphocyte aggregation focus

Then the serpent made a rush at the youth with wide open jaws13 to swallow him at one gulp14, but the young man leaped aside and drew his sword, and fought till he had cut off all the seven heads

(approximately ＞ 50/focus) was observed. Combining with clinical findings, these pathological changes were consistent with Sjogren’s syndrome. Blood and CSF samples were submitted for mNGS test again on January 8, 2021. The results of CSF samples were negative, but 1 unique read of human herpes virus 6B was revealed from the blood sample. qPCR did not detect CMV in either the blood or CSF samples. Head magnetic resonance imaging on January 19, 2021 showed various abnormal symptoms, such as multiple ischemia and degeneration foci in the bilateral frontal lobe, infarction and formation of encephalomalacia foci in the left paraventricular area, and cerebral arteriosclerosis. The conditions improved after continued low-dose hormone and anti-epileptic treatment, and the patient was discharged on January 20, 2021.

DlSCUSSlON

The symptoms improved after antiviral, hormonal, antihypertensive, and neurotrophic treatment.

Intracranial CMV infection can affect the meninges and parenchyma, manifesting different degrees of consciousness disturbance, mental symptoms, seizures, hemiplegia, and sensory disturbances[6]. The epilepsy-type CMV encephalitis always performs non-specifically on EEG, which mainly shows diffused slow waves, accompanied by paroxysmal slow waves and sharp waves[11,12]. In this case, given the history of diabetes, this patient had underlying disease and predisposing factors of immunosenescence. The onset of disease was mainly in the form of epileptic seizure, which subsequently progressed to status epilepticus and accompanied by signs of left side paralysis and unsteady gait. Magnetic resonance imaging indicated high signals on diffusion-weighted imaging sequence of the right postcentral gyrus. mNGS detection of blood and CSF indicated CMV infection, confirmed by qPCR detection using blood samples. The diagnosis was finally confirmed as CMV meningitis of the epilepsy type. After continuously strengthened antiviral and anti-epileptic treatment, the patient’s conditions significantly improved.

In this case, mNGS combined with qPCR provided a rapid and reliable diagnosis of CMV encephalitis with high efficiency, sensitivity, and specificity. This indicated that mNGS could be a reliable approach for the diagnosis of CMV encephalitis.

The patient had a smoking history for several decades, approximately 10 cigarettes/d. He denied a history of alcohol abuse or other bad habits.

It is generally agreed that anti-infective treatment must be initiated immediately as soon as severe central infection is identified. Therefore, rapid and timely diagnosis in central nervous system infection plays a key role for the treatment and prognosis of patients. mNGS was then applied and detected the pathogen as CMV quickly, which was subsequently verified by qPCR. This indicated a great advantage of mNGS in detecting pathogens that are hard to diagnose by conventional methods. Interesting, in blood samples, mNGS detected a total of 614 CMV reads, and qPCR detected CMV at a cycle number of 35. While for CSF samples, mNGS only detected 1 unique read of CMV, but qPCR showed a negative result. The results suggested that mNGS was more likely to be more sensitive than qPCR and could detect trace amounts of pathogens. The patient’s conditions improved after treatments. In the meantime, the negative results of the above reexaminations after improvement of post-treatment symptoms were also consistent with the progression of disease, further supporting the diagnosis of CMV encephalitis.

CONCLUSlON

The diagnosis of CMV encephalitis was difficult. There is no clear standard currently. Virus culture and brain biopsy are the gold standards for the diagnosis of CMV encephalitis, but they are rarely used in clinical practice[8,13]. Combined with brain biopsy and CSF virus isolation, the sensitivity and specificity of conventional PCR can reach 80% and 90%, respectively[8]. However, PCR always needs a prior hypothesis of the target and can only detect gene sequences of known pathogenic microorganisms. In addition, time is crucial for patients with acute and critical CMV encephalitis. A rapid, accurate, and unbiased diagnostic method is needed. mNGS can quickly and accurately analyze all microorganisms in samples, which has extremely high application value in the diagnosis of infectious diseases, especially for unknown pathogens and those with trace amounts[14]. It has been increasingly applied in diagnosing multiple diseases, including encephalitis and meningitis[15]. mNGS shows a high sensitivity and accuracy in the diagnosis of various pathogens, especially for viruses, difficult-to-cultivation bacteria, and fungi[9].

FOOTNOTES

Peng FH, Zhang ZY, and Xu CQ directly participated in planning and execution; Xu CQ, Chen XL, Zhang DS, Wang JW, Yuan H, and Chen WF collected and organized the data; Lou Z and Xia H contributed in metagenomic next-generation sequencing experiment and result analysis; Peng FH, Xu CQ, Chen XL, and Zhang DS analyzed the data; Xu CQ, Chen XL, Zhang DS, and Wang JW drafted and revised the manuscript; all the authors read and approved the final manuscript.

10. First she looked in at the window, and then she peeped in at the keyhole; and seeing nobody in the house, she lifted the latch: Goldilocks doesn t knock on the door first. She cases the joint and then enters when she determines no one is home. These are not the actions of someone planning to do good. These are the actions of someone far from innocent and inexperienced.Return to place in story.

This study was approved by the ethical review committee of First Affiliated Hospital of Anhui Medical University. Written informed consent was obtained from the patient.

All authors declare that they have no conflicts of interest.

The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

China

Chang-Qing Xu 0000-0002-3441-9980; Xia-Ling Chen 0000-0002-2454-7831; Dong-Sheng Zhang 0000-0003-2574-1270; Jia-Wei Wang 0000-0002-2540-2036; Hong Yuan 0000-0002-6592-1519; Wei-Fan Chen 0000-0003-0265-3577; Han Xia 0000-0002-5179-0255; Zhong-Yin Zhang 0000-0001-5515-0004; Fu-Hua Peng 0000-0002-2005-4802.

Liu JH

When I was superintendent1 of schools in Palo Alto, California, Polly Tyner, the president of our board of trustees（）, wrote a letter that was printed in the Palo Alto Times. Polly s son, Jim, had great difficulty in school. He was classified as educationally handicapped and required a great deal of patience on the part of his parents and teachers. But Jim was a happy kid with a great smile that lit up the room. His parents acknowledged his academic difficulties, but always tried to help him see his strengths so that he could walk with pride. Shortly after Jim finished high school, he was killed in a motorcycle accident. After his death, his mother submitted this letter to the newspaper.

Filipodia

Liu JH

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