甲基對硫磷殘留酶聯免疫檢測試劑盒的研制

萬宇平，孫震，馮靜，扶勝，崔廷婷，齊向武

摘要：合成了甲基對硫磷的半抗原，并與載體蛋白偶聯合成了人工抗原，然后通過免疫的方法制備了其單克隆抗體。在篩選甲基對硫磷單克隆抗體的基礎上，建立酶聯免疫檢測方法，并研制出檢測蘋果、玉米、蔬菜中甲基對硫磷殘留的試劑盒。該試劑盒的靈敏度為0.5 μg/L，對蘋果、玉米、蔬菜樣品的最低檢測限分別可達到10、20、50 μg/kg；加標回收率為70.3%～102.4%，變異系數為7.4%～13.0%；對實際樣品的檢測結果與氣相色譜法基本一致。該方法快速、靈敏、可靠，為水果、蔬菜、谷物中甲基對硫磷的殘留檢測提供了便捷、準確的分析手段。

關鍵詞：甲基對硫磷；殘留；酶聯免疫試劑盒

中圖分類號：S482.3；Q511 文獻標識碼：A 文章編號：0439-8114（2013）05-1154-04

Development of an Enzyme-linked Immunosorbent Assay Kit for Detecting of Parathion-methyl Residues

WAN Yu-ping，SUN Zhen，FENG Jing，FU Sheng，CUI Ting-ting，QI Xiang-wu

（Beijing Kwinbon Biotechnology Co. Ltd.，Beijing 102206，China）

Abstract：A hapten artificial antigen was prepared， and monoclonal antibodies against parathion-methyl were produced. On the basis of parathion-methyl monoclonal antibody， an enzyme-linked immunosorbent assay（ELISA） kit for parathion-methyl residues in apple， corn and vegetable was developed. The sensitivity of the ELISA kit was 0.5 μg/L. The detection limits for apple， corn， vegetable were 10， 20， 50 μg/kg， respectively. Sample recoveries were 70.3%～102.4%， and the coefficients of variation were 7.4%～13.0%. Results of the ELISA kit were in accordance with those by gas chromatography（GC）. This method is rapid， sensitive and reliable， which provides a convenient and accurate way for detection of parathion-methyl residues in fruit， cereal and vegetable.

Key words： parathion-methyl； residue； enzyme-linked immunosorbent assay （ELISA） kit