水葫蘆葉中總黃酮的提取及其抗氧化性研究

許麗麗，蔡文軍

摘要：為研究水葫蘆（Eichhornia crassipes）葉中總黃酮的超聲波法提取工藝，并測定其抗氧化活性，通過單因素試驗和正交試驗優化提取條件，對總黃酮還原能力及清除自由基能力進行測定。結果表明，水葫蘆葉中總黃酮最佳提取條件為超聲功率320 W、乙醇濃度70%、超聲時間30 min、料液比1∶45、提取次數3次，此條件下總黃酮得率為5.71%，提取物呈現出良好的抗氧化活性。

關鍵詞：水葫蘆（Eichhornia crassipes）葉；總黃酮；超聲波；提取；抗氧化性

中圖分類號：R284.2 文獻標識碼：A 文章編號：0439-8114（2013）05-1131-03

Study on the Extraction and Antioxidant Activity of Total Flavonoids from Water Hyacinth Leaves

XU Li-li，CAI Wen-jun

（Department of Chemistry， Hanshan Normal University， Chaozhou 521041，Guangdong，China）

Abstract： In order to optimize the ultrasonic extracting technology of the total flavonoids from water hyacinth leaves and determine the antioxidant activity of total flavonoids， the single factor experiments and orthogonal experiment were conducted and the reducing power and free radical scavenging activity were detected. The results showed that the best extracting condition was as follows： ultrasonic power 320 W， treatment time 30 min， alcohol concentration 70%， ratio of material to solvent 1∶45 and extraction 3 times. Under this condition， the extraction rate of total flavonoids was 5.71%. And the extracts showed obvious antioxidant activities.

Key words： water hyacinth leaf； total flavonoids； ultrasonic wave； extraction； antioxidant activity