紫外分光光度法測定大花暗羅葉和枝中總?cè)频暮?/h1>
2013-04-29 19:02:22楊志彬，劉海，郭蘭
湖北農(nóng)業(yè)科學 2013年5期
楊志彬，劉海，郭蘭
摘要：采用紫外分光光度法測定了大花暗羅（Polyalthia rumphii）葉和枝中總?cè)频暮?。以烏蘇酸為對照品，以5%香草醛-冰醋酸和高氯酸系統(tǒng)為顯色劑，在546 nm波長處測定樣品的吸光度。烏蘇酸對照品在0.2～1.6 μg/mL范圍內(nèi)呈良好線性關(guān)系，回歸方程為A=0.160 3C+0.031 2，R2=0.999 4。大花暗羅枝和葉中總?cè)频暮糠謩e為0.446%和0.956%。該方法操作簡便、結(jié)果穩(wěn)定、重復性好、易于推廣。
關(guān)鍵詞：大花暗羅（Polyalthia rumphii）；總?cè)?；紫外分光光度?/p>
中圖分類號：O657.32；R927.2 文獻標識碼：A 文章編號：0439-8114（2013）05-1158-03
Determination of Total Tritepenoidic in Leaves and Stems of Polyalthia rumphii by Ultraviolet Spectrophotometry
YANG Zhi-bin1，LIU Hai2，GUO Lan1
（1. Ji'an City Center Peoples Hospital， Ji'an 343000， Jiangxi， China；
2.College of Pharmacy， Gannan Medical University， Ganzhou 341000， Jiangxi， China）
Abstract： Taking the ursolic acid as the reference， 5% vanillin-acetic acid and perchloric acid as color system， the content of total triterpenoidic in the leaves and stems of Polyalthia rumphii was determined by ultraviolet spectrophotometric method at 546 nm. The calibration curve was linear（R2=0.999 4）in the range of 0.2～1.6 μg/mL，and the curve was A=0.160 3 C+0.031 2，the content of total triterpenoidic in the leaves and stems of P. rumphii was 0.956% and 0.446% respectively. The result was stable and repeatable. This method was operated conveniently and spread easily.
Key words： Polyalthia rumphii； total tritepenoidic； ultraviolet spectrophotometric method
楊志彬，劉海，郭蘭
摘要：采用紫外分光光度法測定了大花暗羅（Polyalthia rumphii）葉和枝中總?cè)频暮?。以烏蘇酸為對照品，以5%香草醛-冰醋酸和高氯酸系統(tǒng)為顯色劑，在546 nm波長處測定樣品的吸光度。烏蘇酸對照品在0.2～1.6 μg/mL范圍內(nèi)呈良好線性關(guān)系，回歸方程為A=0.160 3C+0.031 2，R2=0.999 4。大花暗羅枝和葉中總?cè)频暮糠謩e為0.446%和0.956%。該方法操作簡便、結(jié)果穩(wěn)定、重復性好、易于推廣。
關(guān)鍵詞：大花暗羅（Polyalthia rumphii）；總?cè)?；紫外分光光度?/p>
中圖分類號：O657.32；R927.2 文獻標識碼：A 文章編號：0439-8114（2013）05-1158-03
Determination of Total Tritepenoidic in Leaves and Stems of Polyalthia rumphii by Ultraviolet Spectrophotometry
YANG Zhi-bin1，LIU Hai2，GUO Lan1
（1. Ji'an City Center Peoples Hospital， Ji'an 343000， Jiangxi， China；
2.College of Pharmacy， Gannan Medical University， Ganzhou 341000， Jiangxi， China）
Abstract： Taking the ursolic acid as the reference， 5% vanillin-acetic acid and perchloric acid as color system， the content of total triterpenoidic in the leaves and stems of Polyalthia rumphii was determined by ultraviolet spectrophotometric method at 546 nm. The calibration curve was linear（R2=0.999 4）in the range of 0.2～1.6 μg/mL，and the curve was A=0.160 3 C+0.031 2，the content of total triterpenoidic in the leaves and stems of P. rumphii was 0.956% and 0.446% respectively. The result was stable and repeatable. This method was operated conveniently and spread easily.
Key words： Polyalthia rumphii； total tritepenoidic； ultraviolet spectrophotometric method
