張麗輝++王相威++張智航++陳冊
摘要 為了解鹽脅迫對黑心菊種子萌發(fā)的影響,采用紙上發(fā)芽法研究了不同濃度(0、50、100、150 mmol/L)Na2CO3、NaHCO3、NaCl脅迫對黑心菊種子發(fā)芽率、發(fā)芽勢、發(fā)芽指數(shù)及胚生長的影響。結(jié)果表明:隨著鹽溶液濃度的增加,黑心菊種子的發(fā)芽率、發(fā)芽勢、發(fā)芽指數(shù)呈下降趨勢,當(dāng)鹽濃度達(dá)到150 mmol/L時,NaHCO3和NaCl處理的發(fā)芽勢均等于0。種子對3種鹽的耐受力大小為Na2CO3>NaHCO3>NaCl;不同種類的鹽脅迫對胚根和胚芽的影響不同,但總體表現(xiàn)為高鹽抑制了胚根的生長,反映出胚根是對鹽分脅迫較敏感的部位。
關(guān)鍵詞 黑心菊;種子萌發(fā);鹽脅迫;堿性鹽
中圖分類號 S648 文獻(xiàn)標(biāo)識碼 A 文章編號 1007-5739(2015)17-0190-02
Effects of Salinities Stress on Seed Germination of Rudbeckia hybrida
ZHANG Li-hui WANG Xiang-wei ZHANG Zhi-hang CHEN Ce
(School of Life Sciences,Changchun Normal University,Changchun Jilin 130032)
Abstract In order to understand the effects of salt stress on the seed germination of R. hybrida,the salt tolerance of Rudbeckia hybrida seeds were tested under four salt solutions(0 mmol/L,50 mmol/L,100 mmol/L,150 mmol/L)to research the effect of salt stress on germination rate,germination potential and germination index of Rudbeckia hybrida seeds. The results showed that the germination rate,germination potential and germination index significantly decreased than the control with the increasing of salt concentration. When the NaHCO3 and NaCl concentration were up to 150 mmol/L,the germination potential were 0.The sodium salts were Na2CO3,NaHCO3,NaCl,respectively,based on salt injury rates of seed germination from high to low,under the same concentrations of sodium salts solutions. The radicle and germ had different response to the salts. The salt at higher concentrations could inhibit the growth of radicle.
Key words Rudbeckia hybrida;germination;salt stress;alkaline salt
黑心菊(Rudbeckia hybrida)是菊科金光菊屬多年生草本植物。性喜陽光,既耐寒又耐旱,適應(yīng)性強(qiáng),不擇土壤,是城市園林綠化中重要的觀賞植物之一[1]。種子萌發(fā)是每個植物生活周期的重要階段[2],在此階段,該植物的生理活動極易受到外界環(huán)境的影響[3],從而影響該植物的生長和發(fā)育。鹽脅迫就是影響種子萌發(fā)的重要因素之一,國內(nèi)外諸多研究表明,鹽脅迫抑制植物的生長發(fā)育,降低種子萌發(fā)速率及萌發(fā)的百分率[4-7]。以往的研究多數(shù)集中在中性鹽脅迫[8-11],較少涉及堿性鹽脅迫和二者之間的比較[12-13],目前對黑心菊種子萌發(fā)期間的耐鹽性系統(tǒng)研究未見報道。本試驗對黑心菊種子進(jìn)行不同濃度Na2CO3、NaHCO3、NaCl 3種鹽脅迫處理,研究不同鹽處理下黑心菊種子萌發(fā)的特性,探討黑心菊種子萌發(fā)指標(biāo)及幼苗生長指標(biāo)與鹽脅迫程度之間的關(guān)系,以期為鹽堿地區(qū)的花卉生產(chǎn)提供理論依據(jù)。……p>