雜種藍莓(野生ב美登’)組培快繁1)

甄成　畢顯禹　李淑娟　　　　孫小環　許艷玲　石德山

(林木遺傳育種國家重點實驗室(東北林業大學)，哈爾濱，150040)　　　　(大興安嶺地區農業林業科學研究院)

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雜種藍莓(野生ב美登’)組培快繁1)

甄成畢顯禹李淑娟孫小環許艷玲石德山

(林木遺傳育種國家重點實驗室(東北林業大學)，哈爾濱，150040)(大興安嶺地區農業林業科學研究院)

摘要為了建立高效的雜種藍莓組培快繁技術體系，以大興安嶺地區野生藍莓和栽培品種‘美登’(‘Blomidon’)雜交獲得的雜交種子為試材，篩選出雜交種子最佳無菌處理條件，獲得藍莓雜種無菌苗。再以雜種無菌苗莖段為外植體，探究了不同質量濃度植物生長調節劑(ZT)、蔗糖及繼代周期對叢生芽誘導的影響，并探討了培養基種類及植物生長調節劑IBA質量濃度對生根誘導的影響。結果表明：雜交種子經75%酒精和3%次氯酸鈉分別處理20 s和7 min效果最好，污染率為0；添加0.6 mg·L-1ZT和20 g·L-1蔗糖的WPM培養基為叢生芽誘導最佳培養基，誘導率高達100%，且芽叢密集、長勢好；芽誘導期間，30 d的繼代周期最有利于叢生芽的增殖，增殖倍數高達34.72；最佳生根培養基為添加0.4 mg·L-1IBA的1/2WPM培養基，生根率高達88%，且生根周期僅為40 d，移栽成活率達到85%以上。

關鍵詞藍莓；野生ב美登’；雜交種子；組織培養；快速繁殖

分類號Q943.1

Rapid Propagation System of Hybrid Blueberry (wildבBlomidon’)//

Zhen Cheng, Bi Xianyu, Li Shujuan

(State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, P. R. China); Sun Xiaohuan, Xu Yanling, Shi Deshan(Daxing’an Mountain Academy of Agriculture and Forestry Sciences)//Journal of Northeast Forestry University，2016,44(5)：29-33.

The objective of the study is to establish a rapid propagation system of hybrid blueberry. The wild blueberry of Daxing’an Mountains and the cultivar of ‘Blomidon’ were selected as parents to take intervarietal crosses and the hybrid seeds. The best sterile treatment conditions for hybrid seeds were selected and the sterile seedlings were got. The effects of the concentration of ZT, the contents of source and subculture cycle on frequency of cluster buds induction were conducted by using stem segments of seedlings as explants. The influence of different types of medium and concentration of IBA on the induction of adventitious root was analyzed. The contamination rate of hybrid seeds was 0 after sterilized with 75% ethanol and 3% sodium hypochlorite for 20 s and 7 min, respectively. The best medium for cluster buds induction was WPM containing 0.6 mg·L-1ZT and 20 g·L-1source. The induction rate was up to 100% and the cluster buds grew well. During the bud induction, the optimal subculture cycle for buds proliferation was 30 d with the proliferation coefficient of 34.72. The best rooting medium was 1/2WPM containing 0.4 mg·L-1IBA, the rooting rate was 88%, and the rooting period was only 40 d. The survival rate of transplanting was more than 85%.

KeywordsBlueberry; WildבBlomidon’; Hybrid seed; Tissue culture; Rapid propagation

藍莓是杜鵑花科(Ericaceae)越桔屬(Vacciniodeae)植物的總稱，為多年生灌木或小喬木，因其果實富含抗氧化劑、花色素苷、類黃酮等與清除人體自由基和抗衰老機能密切相關的化合物，深受國內外廣大消費者的追捧，目前已躋身于世界五大健康食品行列，并有世界水果之王的美譽[1-2]。……