血管生長素的差異表達對鑒別前列腺癌雄激素依賴性和非依賴性的臨床意義的研究

田河 南錫浩 于峰 白吉祥 邸彥橙 郭振海 吳影

【摘要】目的：研究雄激素依賴性和非依賴性前列腺癌中血管生長素（ANG）的差異性表達的臨床意義，以了解雄激素非依賴性前列腺癌的發病機制。方法：研究時間為2013年7月至2015年7月，采用體外培養的方式分別培養雄激素依賴性（LNCaP細胞）和非依賴性（PC-3細胞）前列腺癌細胞株，并與正常健康人群進行對比；分別在細胞和組織水平上對ANG水平和表達情況進行分析歸納。結果：前列腺癌患者ANG陽性表達率顯著高于正常健康人群（P<0.05）；雄激素依賴性前列腺癌患者各分期中ANG水平明顯低于非依賴性患者，P<0.05，統計學具有顯著差異意義。結論：ANG在雄激素依賴性前列腺癌向非依賴性前列腺癌轉化中具有主導作用，ANG可能成為腫瘤的標記物。

【關鍵詞】雄激素依賴性前列腺癌；雄激素非依賴性前列腺癌；血管生長素；差異表達

Research of ANG differentially expression in identification of androgen-dependent and independent prostate cancerTIAN He， NAN Xihao△， YU Feng， BAI Jixiang， DI Yancheng， GUO Zhenhai， WU Ying. Department of Urology， Mudanjiang Medical College Hongqi Hospital， Mudanjiang 157011， Heilongjiang， China

【Abstract】Objectives： To study the clinical significance of differential expression of ANG in androgen-dependent and independent prostate cancer in order to understand the pathogenesis of androgen-independent prostate cancer. Methods： From July 2013 to July 2015， by way of in vitro culture， we got androgen-dependent （LNCaP cells） and independent （PC-3 cells） prostate cancer cell lines. The cell sample from healthy people was used as control sample； ANG and expression levels at the cellular and tissue levels were respectively analyzed and summarized. Results： ANG expression in prostate cancer patients were significantly higher than that in normal healthy population （P<0.05）. In various stages， ANG levels of androgen-dependent prostate cancer were significantly lower than that of non-dependent prostate cancer， with statistically significant difference （P<0.05）. Conclusion： ANG has a leading role in the conversion to non-independent prostate cancer in androgen dependent prostate cancer and ANG may become tumor marker.

【Key words】Androgen dependent prostate cancer； Androgen-independent prostate cancer； ANG； Differential expression

【中圖分類號】R737.25【文獻標志碼】A

前列腺癌是西方國家老年男性最常見的惡性腫瘤，在我國發病呈逐年增多的趨勢。盡管去勢治療對于進展期前列腺癌患者是最有效的治療方法，但癌細胞會逐漸發展為雄激素非依賴性，在內分泌治療后一旦演變為激素非依賴性前列腺癌，則對患者的治療和預后帶來極大的困難。前列腺癌細胞獲得激素非依賴性的機制，可能與多種因素有關，雖已成為目前國內外的研究熱點但仍處于探索階段。近來研究顯示[1，2]，血管生長素（angiogenin， ANG）在前列腺癌的發生和發展中扮演重要角色。已有報道前列腺癌患者血清ANG水平高于正常人水平，并且在前列腺癌組織中高表達[3]。國內有研究表明ANG在前列腺癌組織中表達顯著高于前列腺良性增生組織，ANG的表達隨前列腺癌患者臨床分期的升高而上調[4]。……