LW-AFC and its active components ameliorate corticosterone-induced long-term potentiation impairment in mice

Yan HUANG,Dong LI,Wen-xia ZHOU,Yong-xiang ZHANG

(1.State Key Laboratory of Toxicology and Medical Countermeasures,Beijing 100850,China;2.Department of TCM and Neuroimmunopharmacology,Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China）

T2-7

LW-AFC and its active components ameliorate corticosterone-induced long-term potentiation impairment in mice

Yan HUANG1,2,Dong LI1,2,Wen-xia ZHOU1,2,Yong-xiang ZHANG1,2

(1.State Key Laboratory of Toxicology and Medical Countermeasures,Beijing 100850,China;2.Department of TCM and Neuroimmunopharmacology,Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China）

OBJECTIVELW-AFC is extracted from the classical traditional Chinese medicinal prescription-Liuwei Dihuang Decoction.Previous studies have showed that LW-AFC could improve learning&memory ability in amny animal models.In this study,we focused on evaluating the effect of several main active components from LW-AFC(B-B;loganin,LOG;morroniside,MOR;paeoniflorin,PF and stachyose,STA)on LTP.METHODSIn vivo recording of LTP was used in this study to evaluate the effects of LW-AFC and it′s active components on coticorsterone(Cort)induced LTP impairment.RESULTSThe results showed that LW-AFC could ameliorate Cort-induced LTP impairment.The effect of LW-AFC was abolished when the immune function was inhibited.Single administration(ig,ip,icv)of any of the components had no effect on Cort-induced LTP impairment.Consecutively intragastric admin?istration or intraperitoneal injections(chronic administration)of B-B,LOG,MOR or PF for 7 d showed protective effect on Cort-induced LTP impairment.Intragastric administration of STA for 7 d protected LTP from impairment induced by Cort,while there was little improving effect when STA was administrated via intraperitoneal injection.In addition,when the intestinal microbiota was disrupted by applying the antibiotic cocktail,STA showed little protective effect against Cort.CONCLUSIONIn conclusion,LW-AFC and it′s components showed positive effects against cort induced LTP impairment,it seems that all displayed protective effects via indirectly,immune modulation might be the common pathway for all components;the exact pathways are different in each component,B-B,LOG,MOR and PF could be absorbed into the bloods tream and then modulate the peripheral immune function,while STA could not be absorbed and modulates the immune function via modulating intestinal microbiota.Further studies are needed to invesgate the underlying mechanisms and the synergetic effects of all components.

LW-AFC;active components;synaptic plasticity;long-term potentiation;intestinal microbiota

Wen-xia ZHOU,Tel:(010)66931625,E-mail:zhouwx@bmi.ac.cn