Inhibitory effect of baicalein on mice tremor induced by oxotremorine and mechanisms

Yu-lin YANG,Xue ZHANG,Shu-mei WANG,Guan-hua DU

（1.Guangdong Pharmaceutical University,Guangzhou 510006,China；2.Beijing Key Laboratory of Drug Target Research and Drug Screening,Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050,China）

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Inhibitory effect of baicalein on mice tremor induced by oxotremorine and mechanisms

Yu-lin YANG1,2,Xue ZHANG2,Shu-mei WANG1,Guan-hua DU1,2

（1.Guangdong Pharmaceutical University,Guangzhou 510006,China；2.Beijing Key Laboratory of Drug Target Research and Drug Screening,Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050,China）

OBJECTIVETo investigate the anti-tremor effect and mechanism of baicalein on oxotremorine-induced muscle tremor in mice.METHODSThe acute model of muscular tremor was induced by intraperitoneal injection of oxotremorine,and the latency,duration and frequency of muscle tremor in mice were measured immediately;the saliva of mice was measured to reflect the correlation between tremor and peripheral nerve function;the aim of this study was to determine the content of MDA and the activity of GSH-PX,and to investigate the anti-oxidation of mice with tremor model.The activity of acetylcholinesterase(AchE)and acetylcholine transferase(ChAT)can indirectly reflect the level of acetylcholine in the brain.The level of monoamine neurotransmitters in brain tissue was determined by high performance liquid chromatography(HPLC-ECD).RESULTSThe animals in the model group appeared obvious tremoring,salivating and erecting and other symptoms.Compared to the model group,there was no obvious inhibitory effect on the administration of each dose.After 7,14,21 and 28 d of continuous administration,the latency,duration and tremor frequency of tremor mice were significantly shortened,the levels of acetylcholine were significantly decreased,the changes of DOPAC and DA neurotransmitters in the brain of model group were recovered,regulate the dynamic balance of acetylcholine and dopamine in the brain.CONCLUSIONLong-term administration can improve the tremor behavior of mice,the mechanismmay be related to the regulation of neurotransmittersin brain.

baicalein;oxotremorine;acetylcholine;monoamine neurotransmitter;HPLC-ECD

Guan-hua DU,Tel:（010）63165184，E-mail：dugh@imm.ac.cn