Wudang cherry ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice

Jie ZHOU,Fei LI

(Department of Pharmacology,School of Pharmaceutical Sciences,Hubei University of Medicine,Shiyan 442000,China)

T2-36

Wudang cherry ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice

Jie ZHOU,Fei LI

(Department of Pharmacology,School of Pharmaceutical Sciences,Hubei University of Medicine,Shiyan 442000,China)

OBJECTIVETo investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODSThe model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg-1)for 7 d.Water extracts of Wudang cherry at 500 mg·kg-1were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg-1)and allopurinol(20 mg·kg-1)were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the mRNA and protein levels of mouse urate transporter 1(mURAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(mOCT1,mOCT2,mOCTN1 and mOCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immuno?fluorescent assay,respectively.RESULTSWudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal mURAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 mRNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang cherry increased renal mOCT1,mOCT2,mOCTN1 and mOCTN2 mRNA and protein levels,and improved renal impairment in this model.CONCLUSIONWudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.

Wudang cherry;hyperuricemia;uric acid;organic ion transporter

The project supported by National Natural Science Foundation of China(81402994)；Natural Science Foundation of Hubei Province(2014CFB652);and the ″2011 plan″Open Fund Innovation Team Project of Hubei Province(2011JH-2014CXTT08)

Fei LI,Tel:18314797989,E-mail：piaopodexinlifei@163.com