Enhanced anti-cancer effect of gambogic acid by gold nanorod-based delivery

Hong-ye WAN,Jian-li CHEN,Xiao-yan YU,Xiao-ming ZHU

(State Key Laboratory of Quality Research in Chinese Medicine,Macau University of Science and Technology,Macau,China）

T2-35

Enhanced anti-cancer effect of gambogic acid by gold nanorod-based delivery

Hong-ye WAN,Jian-li CHEN,Xiao-yan YU,Xiao-ming ZHU

(State Key Laboratory of Quality Research in Chinese Medicine,Macau University of Science and Technology,Macau,China）

OBJECTIVENanotechnology provides a novel strategy for the delivery of anticancer drugs.In this study,titanium dioxide coated gold nanorod(GNR/TiO2)nanostructures were used as the drug carrier for gambogic acid in order to improve its anticancer effect.METHODSBiocompatibility and cellular uptake of GNR/TiO2nanostructures were studied in human glioblastoma U-87 MGcells.Cell viability was evaluated by ATP assay and calcein AM staining.LysoSensor Green DND-189 and Hoechst 33342 were used to analyze the intracellular location of GNR/TiO2nanostructures.The in vitro anti-cancer effect of gambogic acid loaded nanoparticles was compared with free drug.RESULTSThe results showed that GNR/TiO2nanostructures are biocompatible,and they are localized at the intracel?lular acidic compartments of endosomes and lysosomes.The intracellular drug content delivered via GNR/TiO2nanostructures was 6 fold higher than the free form,thus dramatically enhancing the anticancer effect of gambogic acid.Furthermore,mild photothermal therapy also showed synergistic effect with the drug.CONCLUSIONOur study suggested that GNR/TiO2nanostructures can be considered as a promising anticancer drug carrier.

gold nanorods;titanium dioxide;photothermal therapy;gambogic acid;drug delivery

The project supported by Macao Science and Technology Development Fund(FDCT)(014/2014/A1)

Xiao-ming ZHU,E-mail：xmzhu@must.edu.mo