四物湯及活血、養血藥對慢性阻塞性肺疾病氣道重塑及黏膜免疫的影響

陳靜+程羽+夏永輝+葉志鵬+袁嘉麗

[摘要] 目的 探討四物湯及其活血、養血藥對慢性阻塞性肺疾病（COPD）氣道重塑及黏膜免疫的作用機制。 方法 8周齡SPF級Wistar雄性大鼠50只，按體重分層隨機分為正常對照、模型組、四物湯組、活血藥對組、養血藥對組，每組各10只。采用煙熏加脂多糖滴注復制COPD大鼠模型，四物湯組、活血藥對組、養血藥對組于造模成功后給予對應中藥2 mL/（只·d）灌胃，連續4周，模型組給予等量生理鹽水灌胃。正常對照組自由飲水。末次給藥24 h后處死各組大鼠，取左肺進行病理檢查，取右肺組織勻漿以ELISA法檢測分泌型免疫球蛋白A（SIgA）、分泌片（SC）、轉化生長因子β1（TGF-β1）、血管內皮生長因子（VEGF）的表達量。 結果 模型組大鼠肺組織病理顯示，炎性細胞浸潤明顯，氣道結構紊亂，管腔狹窄，氣道壁纖維組織增生明顯，管壁增厚，肺泡腔擴張，肺泡腔融合；藥物干預后，四物湯、活血、養血藥對各組肺組織病理均有不同程度改善。模型組SIgA、SC、TGF-β1、VEGF的表達量較正常對照組顯著升高（P < 0.01）；藥物干預各組較模型組SIgA、SC、TGF-β1、VEGF均顯著降低（P < 0.01）。 結論 SIgA、SC、TGF-β1、VEGF參與COPD的發生發展過程；四物湯、活血、養血藥對可通過調節SIgA、SC、TGF-β1、VEGF的表達干預COPD黏膜免疫平衡及氣道重塑進程。

[關鍵詞] 四物湯；活血藥對；養血藥對；氣道重塑；黏膜免疫

[中圖分類號] R563 [文獻標識碼] A [文章編號] 1673-7210（2017）03（a）-0008-05

Effects of Siwu Tang and Huoxue， Yangxue drugs on airway remodeling and mucosal immunity in rats with chronic obstructive pulmonary disease

CHEN Jing CHENG Yu XIA Yonghui YE Zhipeng YUAN Jiali

School of Basic Medical Science， Yunnan University of Traditional Chinese Medicine， Yunnan Province， Kunming 650500， China

[Abstract] Objective To explore the mechanism of Siwu Tang and Huoxue， Yangxue drug pairs in airway remodeling and mucosal immunity of rats with chronic obstructive pulmonary disease （COPD）. Methods 50 Wistar male healthy rats of SPF grade aged 8 weeks old were randomly divided into the normal control group， COPD model group， Siwu Tang group， Huoxue drug pair group， Yangxue drug pair group， according to the body weight of rats. The rats model of COPD was induced by smoke and lipopolysaccharide （LPS） infusion. The normal control group was given free drank water. After the model successfully established， the corresponding Chinese medicine 2 mL/（rat·d） was given by gavage in the drug groups for 4 weeks. The model group was given normal saline by the same way. 24 h after last administration， the rats of each group were sacrificed and the specimens were collected. Subsequently the pathological changes of the lung tissue were observed， and the levels of secretory immunoglobinA （SIgA）， secretory component （SC）， transforming growth factor-β1 （TGF-β1）， vascular endothelial growth factor （VEGF） in lung tissue were detected by ELISA. Results The pathology of rats lung tissue showed that airway inflammatory cells infiltrated in airway tissue， vessel stenosis， bronchial smooth muscle increased thickness， alveolar dilation and fusion in COPD model group； the pathology of the lung tissue changed better in Siwu Tang group， Huoxue drug pair group and Yangxue drug pair group. Compared with the normal control group， the levels of SIgA， SC， TGF-β1， VEGF increased in the COPD model group （P < 0.01）； after using corresponding traditional Chinese medicine， compared with the COPD model group， the levels of SIgA， SC， TGF-β1， VEGF decreased significantly （P < 0.01）. Conclusion SIgA， SC， TGF-β1， VEGF involves in the development of COPD. Siwu Tang， Huoxue and Yangxue drug pairs improve the mucosal immune balance and airway remodeling process in COPD by regulating the level of SIgA， SC， TGF-β1， VEGF.