熱應激對兔下丘腦-垂體-睪丸軸NMB及其受體基因表達和睪酮分泌的影響

馬志禹 張瑩 陳風雷 楊盛 喬文娜 胡佳慧 張金龍 雷治海

摘要：研究熱應激對公兔下丘腦-垂體-睪丸（HPT）軸神經介素B（NMB）及其受體（NMBR）基因表達水平及血清皮質醇和睪酮分泌的影響，探討熱應激對神經肽和相關激素分泌的影響。建立熱應激模型，采用熒光定量PCR（RT-PCR）檢測兔NMB和NMBR mRNA在HPT軸的表達情況，采用放射免疫分析法（RIA）檢測兔血清中皮質醇和睪酮濃度的變化規律。結果表明，兔NMB和NMBR基因在HPT軸中表達;NMB和NMBR mRNA在HPT軸的表達模式相似，且熱應激后在一定程度上可以促進NMB和NMBR基因的表達，并按時序在下丘腦（熱應激0.5 h）、垂體（熱應激1.0 h）和睪丸（熱應激2.0 h）促進作用最為明顯;熱應激可以促進皮質醇的分泌，并抑制睪酮的分泌，但這種抑制作用隨著熱應激時間的增加（熱應激2.0 h）逐漸恢復正常。表明熱應激可能通過NMB/NMBR系統調節HPT軸的功能，進而影響動物繁殖。

關鍵詞：熱應激;神經介素B;NMBR;下丘腦-垂體-睪丸軸;睪酮

中圖分類號：Q78? ? ? ? ?文獻標識碼：A

文章編號：0439-8114（2019）12-0141-06

DOI：10.14088/j.cnki.issn0439-8114.2019.12.033? ? ? ? ? ?開放科學（資源服務）標識碼（OSID）：

Abstract： The aim of this study was to detect the effect of heat stress on the expression levels of Neuromedin B （NMB） and its receptor （NMBR） genes in the hypothalamus-pituitary-testis（HPT） axis of male rabbit， and the secretion of serum cortisol （Cor.） and testosterone （T）. Establishment The heat stress model was established， the expression levels of NMB and NMBR genes in rabbit HPT axis were detected by real-time PCR（RT-PCR）， and the concentration of Cor. and T in rabbit serum were tested using radioimmunoassay （RIA）. Results showed that NMB and NMBR genes were expressed in rabbit HPT axis. The expression pattern of NMB and NMBR mRNA in HPT axis were similar， and heat stress could promote the NMB and NMBR gene expression， which the role was mostly obvious according to the time sequence in the hypothalamus （heat stress 0.5 h）， pituitary （heat stress 1.0 h） and testis （heat stress 2.0 h）; RIA results indicated that heat stress could improve the concentration of Cor. and inhibit T secretion which the inhibition was gradually weakened over time of heat stress. The results indicated that heat stress may regulate the HPT axis through the NMB/NMBR system to affect reproductive function， and then affected the reproduction of animals.

Key words： heat stress; neuromedin B; neuromedin B receptor; hypothalamus-pituitary-testis axis; testosterone

收稿日期：2018-11-05

作者簡介：馬志禹（1988-），男，河南許昌人，講師，博士，從事神經肽及其受體、神經內分泌與生殖等方面的研究，