中國橙衣屬和果衣屬地衣的新記錄種(英文）

王春曉 孫美潔 趙遵田

摘?要：通過對采自云南省景東彝族自治縣無量山地衣標本的研究，發現了橙衣屬的一個中國新記錄Caloplaca indica（三室類型孢子）和果衣屬的一個中國新記錄種Ramboldia haematites。R.haematites在福建和廣西同樣存在。該文詳細描述了其形態學、解剖學和化學方面的特征，并且提供了顯微結構照片。本研究豐富了云南、福建和廣西地衣的物種多樣性，為橙衣屬和果衣屬地衣分類學研究提供基礎數據和可靠資料。所用標本存放在山東師范大學植物標本室（SDNU）。

關鍵詞：分類學， 地衣型真菌， 東亞

Jingdong Yi Autonomous County is located in Southwest Yunnan of China.It has a subtropical monsoon climate and a variation from 795 to 3 371 m above sea level.The annual average relative humidity is 77%， and the annual average rainfall is 1 086.7 mm.

Caloplaca Th.Fr.s.lat.（Teloschistaceae， Teloschistales， Lecanoromycetes， Ascomycota） is a genus characterized by a crustose or placodioid thallus.There are about 350 species worldwide （Lücking et al.， 2016）.The species usually have orange， K+ purple （anthraquinones present） apothecia， but the most important delimitation is the presence of polarbilocular ascospores.In 1881， Müller placed a group of species bearing plurilocular ascospores （3-6 locules） under Caloplaca section Triophthalmidium.This section is mostly distributed in tropical and subtropical regions of the world and is mainly corticolous， rarely saxicolous （Smith， 2009; Joshi et al.， 2014）.

The genus Ramboldia （Ramboldiaceae， Lecanorales， Lecanoromycetes， Ascomycota） was established by Kantvilas & Elix （1994）.This genus is characterized by a crustose thallus with lecideoid apothecia， Lecanora-type asci， anastomosing paraphyses， and simple and persistently hyaline ascospores （Kantvilas & Elix， 1994， 2007; Elix， 2004）.Some species have orange to red-pigmented apothecia due to the presence of russulone and related anthraquinones （Kantvilas & Elix， 1994; Kalb et al.， 2008）.Ramboldia contains about 30 species worldwide （Lücking et al.， 2016）.In China， only four Ramboldia species， R.cinnabarina， R.elabens， R.heterocarpa and R.russula， have been reported （Zahlbruckner， 1930; Abdulla & Wu， 1998; Aptroot & Sparrius， 2003; Obermayer， 2004）.

The purpose of our study was to enrich knowledge of the species composition of Caloplaca and Ramboldia in China， contributing fundamental data and reliable results for the preparation of a Lichen Flora of China.In this paper， two species of Caloplaca indica and Ramboldia haematites are reported for the first time from China， and Ramboldia is reported for the first time from Fujian and Guangxi.

1?Materials and Methods

The specimens studied were deposited in SDNU （Lichen Section of Botanical Herbarium， Shandong Normal University）.The morphological and anatomical characters of the specimens were examined under a stereo-microscope （Olympus SZ） and a polarizing microscope （Olympus CX21）.Thallus and medulla were spot-tested with K（a 10% aqueous solution of potassium hydroxide）， C（a saturated solution of aqueous sodium hypochlorite）， I （Lugols iodine） and P （a saturated solution of p-phenylenediamine in 95% ethyl alcohol）.The lichen substances were identified using thin layer chromatography （TLC） with solvent system C （Orange et al.， 2010）.Photos of the lichens were taken under Olympus SZX16 and BX61 with DP72.

2?Taxonomic Descriptions

Caloplaca indica Y.Joshi， Jagad.Ram & G.P.Sinha， in Joshi， Jagadeesh， Singh & Sinha， National Academy Science Letters 37（6）：517 （2014）

Fig.1

Morphology—Thallus crustose， corticolous， whitish grey to greenish grey.Photobiont layer continuous.Prothallus ± present， forming a black line at borders.Apothecia zeorine， 0.25-1 mm diameter; disc brown， flat; proper margin thin， flush to raised above the level of disc， concolorous or slightly darker than disc; thalline margin thin， smooth to crenulate， concolorous with thallus.Epithecium yellowish brown， 25-32.5 μm high， K-; hymenium colorless， 75-100 μm high， oil droplets present; hypothecium colorless， oil droplets ± present.Asci clavate， 50-57.5 × 15-20 μm， Teloschistes-type， 8-spored.Ascospores hyaline， bi-?to trilocular， slightly constricted in the centre， all locules of ± equal size， 20-27.5 × 10-13 μm.Pycnidia not seen.On bark.

Chemistry — Thallus and medulla K-， C-， KC-， Pd-， UV+ yellowish orange.Apothecia K-， C-， KC-， Pd-.Lichexanthone detected by TLC.

Distribution — The species is reported so far only from East Himalaya （Joshi et al.， 2014）.New to China.

Specimens examined：CHINA.Yunnan：Jingdong， Wuliangshan， alt.2 200-2 300 m， on bark，7 Aug.2017， R.Tang， M.J.Sun， S.K.Yan & J.M.Fu 20170874， 20170667 （SDNU）.

Comments—Caloplaca indica is mainly characterized by the greyish UV+ yellowish orange （lichexanthone present） thallus， zeorine apothecia， bi-to trilocular ascospores.In terms of ascospore morphology， the species resembles Caloplaca crocea （Kremp.） Hafellner & Poelt and C.trilocularis Zahlbr.C.crocea differs mainly in having yellow to yellowish orange apothecia.C.triloculans differs mainly in having crystals in amphithecium and a thick thalline margin （Joshi et al.， 2014）.

Ramboldia haematites （Fée） Kalb， Lumbsch & Elix， in Nova Hedwigia 86 （1-2）：33 （2008）

Fig.2

Morphology — Thallus grey or greyish-green， fre-quently continuous or smoothly rimose， 90-150 μm thick.No vegetative propagules present.Prothallus not seen.Apothecia adnate， roughly round， flat， 0.15-1 mm diameter; disc orange-red， pruina absent; exciple red， K+ purple; epithecium 10-14 μm thick， reddish; hymenium 35-45 μm thick， colourless; subhymenium 20-35 μm thick， colorless; hypothecium 55-100 μm thick， pale brown.Asci clavate， 37.5-45 × 10-15 μm， Lecanora-type， 8-spored; Ascospores colourless， simple， ellipsoid， 8-10 × 3-4 μm.Pycnidia not seen.On bark.

Chemistry—Thallus and medulla K+ yellow to red or K-， C-， UV+ yellow.Lichexanthone， secalonic acid A （±）， norstictic acid and russulone detected by TLC.

Distribution—This species is known from North America， Africa， Australia， New Caledonia （Kalb et al.， 2008; Elix， 2009） and Japan （Yamamoto et al.， 2013）.New to China.

Specimens examined：CHINA.Yunnan：Jingdong， Wuliangshan， alt.2 200 m， on bark， 7 Aug.2017， R.Tang， M.J.Sun， S.K.Yan & J.M.Fu 20170679， 20170847 （SDNU）.Guangxi：Baise， Cenwanglaoshan， alt.1 800 m， on bark， 24 Feb.2011， L.Li 20111740 （SDNU）.Fujian：Longyan， Huanlianyu， alt.1 400 m， on bark， 29 Oct.2010， D.F.Jiang 20105509 （SDNU）.

Comments—Ramboldia haematites is morphologi-cally similar to Ramboldia russula （Ach.） Kalb， Lumbsch & Elix， but differs in having norstictic acid （not fumarprotocetraric acid） in the apothecia （Kantvilas & Elix， 1994， 2007; Elix， 2004; Kalb et al.， 2009; Gumboski， 2014）.Our specimens are very similar to the original description of R.haematites （Elix， 2009）， except that we failed to detected any connorstictic acid， which was considered one of the taxonomically important characteristics for the species by Kalb et al.（2008）.

Acknowledgements?We thank Dr.Yogesh Joshi （Lichenology Division， Department of Botany， Kumaun University， S.S.J.Campus， Almora， Uttarakhand， India） and Dr.John A.Elix （Research School of Chemistry， Australian National University， Canberra， Australia） for providing great help during the study.

References：

ABBAS A， WU JN， 1998.Lichens of Xinjiang[M].Xinjiang：Sci-Technol & Hygiene Publishing House：85.

APTROOT A， SPARRIUS LB， 2003.New microlichens from Taiwan[J].Fungal Divers， 14（1）：1-50.

ELIX JA， 2004.Ramboldia[M]// MCCARTHY PM， MALLETT K.Flora of Australia：Volume 56A Lichens 4.Victoria：CSIRO Publishing：63-68.

ELIX JA， 2009.Ramboldia[M]// MCCARTHY PM.Flora of Australia：Volume 57 Lichens 5.Victoria：CSIRO Publishing：19-31.

GUMBOSKI EL， 2014.Contribution to the knowledge of lichenized mycota from Southern Brazil.I—Species of Ramboldia （Ascomycota：Lecanoraceae）[J].Acts Biol Catarinense， 1（2）： 60-66.

JOSHI Y， JAGADEESH RT， SINGH P， et al.， 2014.Caloplaca indica， a new lichenized Ascomycetes （Teloschistaceae） from Eastern Himalaya， India[J].Natl Acad Sci Lett， 37（6）：517-519.

KALB K， BUARUANG K， PAPONG K， et al.， 2009.New or otherwise interesting lichens from the tropics， including the lichen genus Ramboldia in Thailand[J].Mycotaxon， 110（1）：109-123.

KALB K， STAIGER B， ELIX JA， et al.， 2008.A new circumscription of the genus Ramboldia （Lecanoraceae， Ascomycota） based on morphological and molecular evidence[J].Nova Hedwigia， 86（1-2）：23-42.

KANTVILAS G， ELIX JA， 1994.Ramboldia， a new genus in the lichen family Lecanoraceae[J].Bryologist， 97：296-304.

KANTVILAS G， ELIX JA， 2007.The genus Ramboldia （Lecanoraceae）：A new species， key and notes[J].The Lichenologist， 38（2）：135-141.

LKING R， HODKINSON BP， LEAVITT SD， 2016.The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota — Approaching one thousand genera[J].Bryologist， 119（4）， 361-416.

OBERMAYER W， 2004.Additions to the lichen flora of the Tibetan region[J].Bibl Lichenol， 88：479-526.

ORANGE A， JAMES PW， WHITE FJ， 2010.Microchemical methods for the identification of lichens[M].2nd ed.London：British Lichen Society.

SMITH CW， 2009.Lichens of Great Britain and Ireland[J].Brit Lichen Soci：245-273.

YAMAMOTO Y， KUROGI S， HARA K， et al.， 2013.Ramboldia haematites （Fée） Kalb et al.new to Asia[J].Lichenology， 11（2）：67-71.

ZAHLBRUCKNER A，1930.Lichenes in Handel-Mazzetti， Symbolae SinicaeⅢ[M].Austria：Julius Springer In Vienna：108.

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