接種叢枝菌根真菌對辣椒積累轉運鎘的影響

牟玉梅　邢丹　周鵬　宋拉拉　吳康云　胡明文　蓋霞普

摘要：【目的】明確叢枝菌根真菌對辣椒植株積累轉運鎘（Cd）的影響，為阻控Cd向辣椒植株地上部轉移和植物修復的應用提供參考依據?！痉椒ā坎捎门柙栽囼?，以辣研201為試材，對其接種根內根生囊霉（Rhizophagus intraradices）和摩西管柄囊霉（Funneliformis mosseae），比較接種與未接種的土壤、辣椒根系與地上部Cd含量變化，從而探究接種根內根生囊霉和摩西管柄囊霉對辣椒植株積累轉運Cd的影響。【結果】與來接種處理相比，接種根內根生囊霉和摩西管柄囊霉后土壤中Cd總量顯著提高10.80%和9.98%（P<0.05，下同），土壤中Cd各形態含量占比發生變化，殘渣態Cd含量提高79.82%和95.44%，而酸溶態Cd含量占比的遷移風險降低32.19%和29.45%。接種Ri和Fm后辣椒根系Cd含量分別顯著增加39.57%和53.13%，根系富集系數顯著分別提高33.15%和40.68%;而地上部Cd含量分別降低15.02%和9.30%，地上部富集系數分別顯著降低24.35%和18.91%;辣椒體內Cd轉運系數分別顯著下降30.15%和33.72%?；诘湫拖嚓P分析可知，典型權重較大的殘渣態Cd含量對土壤Cd含量變化起正向作用，辣椒根系Cd富集系數與體內Cd轉運系數對辣椒Cd含量變化起反向作用;基于典型載荷與交叉載荷分析可知，土壤中酸溶態Cd低則移除程度弱、殘渣態Cd高則滯留程度強，因而辣椒積累轉運Cd能力差?！窘Y論】接種從枝菌根真菌可調控辣椒地上部與根系間Cd的分配，一定程度上可控制土壤中的Cd由根部向地上部轉移。

關鍵詞： 辣椒;叢枝菌根真菌;鎘;富集能力;轉運能力

中圖分類號： S641.3? ? ? ? ? ? ? ? ? ? ? ? ? ? 文獻標志碼： A 文章編號：2095-1191（2021）01-0172-08

Abstract：【Objective】To investigate the effects of arbuscular mycorrhizal fungi on cadmium（Cd） accumulation and transport in Capsicum annum L.（pepper）， so as to provide reference for the application of preventing Cd transfer to the above-ground part of pepper and phytoremediation. 【Method】The experiment was carried out with Layan 201 as the material and planted in pot， pepper was inoculated Rhizophagus intraradices and Funneliformis mosseae respectively. Analyzed the variation of Cd content in soil with and without inoculation， pepper root and aboveground part， and explored and discussed the effects of inoculating R. intraradices and F. mosseae on accumulation and transport Cd in pepper. 【Result】Compared with the non-inoculation treatment，? the total content of Cd in soil with the R. intraradices and F. mosseae inoculated significantly increased by 10.80% and 9.98%（P<0.05， the same below）， and the proportion of Cd in different forms in soil changed. The residual Cd increased by 79.82% and 95.44%. While， with regard of the proportion of acid-so-luble Cd， the migration risk decreased by 32.19% and 29.45%. With R. intraradices and F. mosseae inoculated in soil， Cd content in the root system increased by 39.57% and 53.13%， and Cd enrichment coefficient in the root system increased by 33.15% and 40.68%， while Cd content in the aboveground part decreased by 15.02% and 9.30%. Cd enrichment coefficient in the aboveground part decreased by 24.35% and 18.91% and Cd transport coefficient in pepper body decreased by 30.15% and 33.72%. Based on canonical correlation analysis， residual state Cd with a large typical weight had a positive effect on the change of Cd content in soil， and Cd enrichment coefficient in root and Cd transport coefficient in pepper had negative effect on the change of Cd content. For the analysis of typical loading and cross loading， Cd in low acid- so-luble state in soil resulted in weak removal degree， while Cd in high residual state resulted in strong retention degree， so pepper had poor ability to accumulate and transfer Cd. 【Conclusion】The distribution of Cd between aboveground part and root of pepper is controlled by inoculation of mycorrhizal fungi， and it can inhibit the transfer of Cd in soil from root to aboveground part.

徐笠，陸安祥，田曉琴，何洪巨，殷敬偉. 2017. 典型設施蔬菜基地重金屬的累積特征及風險評估[J]. 中國農業科學，50（21）： 4149-4158. [Xu L，Lu A X，Tian X Q，He H J，Yin J W. 2017. Accumulation characteristics and risk assessment of heavy metals in typical greenhouse vegetable bases[J]. Scientia Agricultura Sinica，50（21）： 4149-4158.]

張文麗，姚丹成，孫嘉龍，韓京秀，尚琪. 2015. 貴州省赫章縣鎘污染區人群健康損害狀況調查及損害指標關系探討[J]. 衛生研究，44（5）：780-787. [Zhang W L，Yao D C，Sun J L，Han J X，Shang Q. 2015. Survey on the status of health effects and relationship between indices of health effects of population in environment cadmium po-lluted area in Hezhang County，Guizhou Province[J]. Journal of Hygiene Research，44（5）：780-787.]

Aloui A，Recorbet G，Gollotte A，Robert F，Valot B，Gianina-zzi-Pearson V，Aschi-Smiti S，Dumas-Gaudot E. 2009. On the mechanisms of cadmium stress alleviation in Medicago truncatula by arbuscular mycorrhizal symbiosis：A root proteomic study[J]. Proteomics，9（2）：420-433.

Bissonnette L，St-Arnaud M，Labrecque M. 2010. Phytoextraction of heavy metals by two Salicaceae clones in symbiosis with arbuscular mycorrhizal fungi during the second year of a field trial[J]. Plant and Soil，332：55-67

Chaturvedi R，Favas P，Pratas J，Varun M，Paul M S. 2018. Assessment of edibility and effect of arbuscular mycorrhizal fungi on Solanum melongena L. grown under heavy metal（loid） contaminated soil[J]. Ecotoxicology and Environmental Safety，148：318-326.

Chen B D，Liu Y，Shen H，Li X L，Christie P. 2004. Uptake of cadmium from an experimentally contaminated calca-reous soil by arbuscular mycorrhizal maize（Zea mays L.）[J]. Mycorrhiza，14：347-354.

Coninx L，Martinova V，Rineau F. 2017. Mycorrhiza-assisted phytoremediation[J]. Advances in Botanical Research，83：127-188.

González-Chávez M C，Carrillo-González R，Wright S F，Ni-chols K A. 2004. The role of glomalin，a protein produced by arbuscular mycorrhizal fungi，in sequestering potentially toxic elements[J]. Environmental Pollution，130（3）：317-323.

Hassan S E，Hijri M，St-Arnaud M. 2013. Effect of arbuscular mycorrhizal fungi on trace metal uptake by sunflfl ower plants grown on cadmium contaminated soil[J]. New Biotechnology，30（6）： 780-787.

Hu J L，Tsang W，Wu F Y，Wu S C，Wang J H，Lin X G，Wong M H. 2016. Arbuscular mycorrhizal fungi optimize the acquisition and translocation of Cd and P by cucumber（Cucumis sativus L.） plant cultivated on a Cd-conta-minated soil[J]. Journal of Soils and Sediments，16：2195-2202.

Joner E J，Briones R，Leyval C. 2000. Metal-binding capacity of arbuscular mycorrhizal mycelium[J]. Plant and Soil，226：227-234.

Koide R T，Mosse B. 2004. A history of research on arbuscular mycorrhiza[J]. Mycorrhiza，14：145-163.

Kuga Y，Zhang X，Wu S L，Ohtomo R. 2018. Uptake and intra-radical immobilization of cadmium by arbuscular mycorrhizal fungi as revealed by a stable isotope tracer and synchrotron radiation μX-ray fluorescence analysis[J]. Microbes and Environments，33（3）：257-263.

Liu Y Z，Xiao T F，Baveye P C，Zhu J M，Ning Z P，Li H J. 2015. Potential health risk in areas with high naturally-occurring cadmium background in southwestern China[J]. Ecotoxicology and Environmental Safety，112：122-131.

Mnasri M，Janou?ková M，Rydlová J，Abdelly C，Ghnaya T. 2017. Comparison of arbuscular mycorrhizal fungal effects on the heavy metal uptake of a host and a non-host plant species in contact with extraradical mycelial network[J]. Chemosphere，171：476-484.

Repetto O，Corre G B，Dumas-Gaudot E，Berta G，Gianinazzi-Pearson V，Gianinazzi S. 2003. Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots[J]. New Phytologist，157（3）：555-567.

Wang F Y，Shi Z Y，Xu X F，Wang X G，Li Y J. 2013. Contribution of AM inoculation and cattle manure to lead and cadmium hytoremediation by tobacco plants[J]. Environmental Science：Processes & Impacts. doi：10.1039/c3em3 0937a.

Xin J L，Huang B F，Liu A Q，Zhou W J，Liao K B. 2013. Identification of hot pepper cultivars containing low Cd levels after growing on contaminated soil：Uptake and redistribution to the edible plant parts[J]. Plant and Soil，373：415-425.

Zhang X Y，Chen D M，Zhong T Y，Zhang X M，Cheng M，Li X H. 2015. Assessment of cadmium（Cd） concentration in arable soil in China[J]. Environmental Science and Pollution Research，22： 4932-4941.

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