野生桑葚中花色苷成分分析

陳 亮，辛秀蘭，袁其朋

(1.北京化工大學(xué)化工資源有效利用國家重點實驗室，北京100029;2.北京電子科技職業(yè)學(xué)院，北京100029)

野生桑葚中花色苷成分分析

陳 亮1，2，辛秀蘭2，*，袁其朋1，*

(1.北京化工大學(xué)化工資源有效利用國家重點實驗室，北京100029;2.北京電子科技職業(yè)學(xué)院，北京100029)

運用固相萃取純化技術(shù)與高效液相色譜/二極管陣列檢測器/電噴霧質(zhì)譜聯(lián)用技術(shù)，采用Zorbax SB-C18色譜柱(250mm×4.6mm，5μm)，甲醇-5%甲酸水溶液為流動相，流速為1.0mL/min，檢測波長為520nm，以矢車菊素3-葡萄糖苷為對照，外標法測定了野生桑葚花色苷含量，并通過紫外掃描光譜和電噴霧質(zhì)譜正離子碎片信息確定了花色苷的成分組成。結(jié)果表明:野生桑葚總花色苷含量為154.27mg/100g，含有的三種花色苷成分分別為矢車菊素3-葡萄糖苷、矢車菊素3-蕓香糖苷和天竺葵素3-葡萄糖苷，其相對含量為67.52%、31.29%和1.06%。

野生桑葚，花色苷，固相萃取，高效液相色譜-質(zhì)譜聯(lián)用

Abstract:To establish a method to determine anthocyanin content and composition in wild mulberry，solid phase extraction(SPE)and high performance liquid chromatography equipped with a DAD detector and electro-spray ionization mass spectrometry(HPLC/DAD/ESI/MS)were used.The analysis was carried on Zorbax SB-C18column(250mm ×4.6mm，5μm).The mobile phase was aqueous 5%formic acid solution and methanol at the flow-rate of 1.0mL/min，the detection wavelength was 520nm.The content of gross anthocyanins was 154.27mg/100g by HPLC expressed as milligrams of cyanidin 3-glucoside equivalent per 100g of fresh weight.Three anthocyanins in wild mulberry were identified by UV-Vis and ESI+-MS spectra which were cyanidin 3-glucoside，cyanidin 3-rutinoside and pelargonidin 3-glucoside，the relative content of which were 67.52%，31.29%，1.06%respectively.

Key words:wild mulberry;anthocyanin;solid phase extraction;high performance liquid chromatography-mass spectrometry(HPLC/MS)

桑葚(Mulberry)是桑科桑屬落葉喬木桑樹Morusalba Linn.的果實，曬干或蒸后曬干可入藥，味甘酸，性寒，歸心、肝、腎經(jīng)，中醫(yī)可用于治療肝腎不足和血虛精虧導(dǎo)致的頭暈?zāi)垦！⒀岫Q、失眠多夢、津傷口渴等癥。桑葚酸甜適口，營養(yǎng)豐富，富含鞣酸、蘋果酸、多種維生素和人體必需的氨基酸及鋅、鉀、鎂、磷等微量礦質(zhì)元素，具有良好的保健功能，很早就被當(dāng)作水果和中藥材加以應(yīng)用［1］。此外，桑葚顏色紫紅，含有大量的天然紅色素。桑葚紅色素屬花色苷類成分，是果蔬及其產(chǎn)品呈現(xiàn)從紅色到藍色的物質(zhì)基礎(chǔ)，具有抑制血小板凝固，預(yù)防血栓、心臟病，抗癌，延緩衰老等作用［2-4］。……