陳東宇,湯曉艷,孫京新
(1.青島農業大學,山東青島266109;2.中國農業科學院農業質量標準與檢測技術研究所,農業部農產品質量安全重點實驗室,北京100081)
過氧乙酸快速檢測試紙條研制與應用
陳東宇1,2,湯曉艷2,*,孫京新1,*
(1.青島農業大學,山東青島266109;2.中國農業科學院農業質量標準與檢測技術研究所,農業部農產品質量安全重點實驗室,北京100081)
分別用定性濾紙、定量濾紙、微孔濾膜為試紙條載體制作了過氧乙酸快速檢測試紙條,并制成標準比色卡對過氧乙酸消毒液進行含量檢測。結果顯示,微孔濾膜為最適宜制作過氧乙酸快速檢測試紙的材質,最適烘干溫度為55℃,最適烘干時間為15min,最適保存條件為4℃下密封貯藏保存21d。利用微孔濾膜制作的檢測試紙條能夠在30s內對消毒液中的過氧乙酸濃度進行檢測,最低檢出限為質量百分比0.1%。
過氧乙酸,試紙條,快速檢測
Abstract:Qualitative filter paper,quantitative filter paper,microporous filter membrane as the carrier were used to produce a rapid detection test strip of peracetic acid solution,and standard color card was made for testing the content of peracetic acid.The results showed that microporous membrane was the most suitable material for making the rapid detection test strip of peracetic acid solution,and the optimum drying temperature was 55℃,optimum drying time was 15min,optimum storage conditions was 4℃ and keeping sealed for 21 days.The microporous membrane test strip could be used within 30s to test the concentration of peracetic acid solution.The minimum detection limit was 0.1%(expressed as mass percentage).
Key words:peracetic acid;test strip;rapid detection
過氧乙酸(PAA)別名過醋酸,目前在醫療、工業用水、食品企業等領域的消毒方面得到廣泛應用[1]。在食品領域,過氧乙酸溶液主要被用于食品冷凍庫及食品用工具、設備的消毒[2-3]。用于保鮮消毒的低濃度過氧乙酸也不穩定,易分解揮發,使用前需進行濃度測定。目前國家標準檢測過氧乙酸溶液濃度的方法為碘量法[4],此外其他檢測方法包括比色法[5]、熒光法[6]、電化學法[7]、分光光度法[8]及色譜法[9-10]。
碘量法測定過氧乙酸含量的過程非常繁瑣,得出結果較慢;其他方法均需要專門的設備和試劑,成本高,操作復雜,不便于現場推廣應用。……