王江波,吳翠云,張銳
摘要:采用SSAP分子標記技術對田間自然產生的紅富士蘋果(Malus domestica)新芽變材料及其對照進行了遺傳鑒定。結果表明,一個376 bp DNA片段為芽變材料特異性片段,其序列與蘋果查爾酮合成酶(CHS)基因啟動子同源性達90%,表明芽變材料是遺傳物質發生改變的穩定變異,為進一步研究芽變材料發生性狀變異的分子機理奠定基礎。
關鍵詞:蘋果(Malus domestica);芽變;SSAP;分析
中圖分類號:Q789;S661.1 文獻標識碼:A 文章編號:0439-8114(2013)05-1181-04
SSAP Analysis of Red Fuji Apple Bud Mutation
WANG Jiang-bo1a,2,WU Cui-yun1a,2,ZHANG Rui1b,2
(a.College of Plant Science;b. College of Life Science,Tarim University, Alar 843300, Xinjiang, China;2.Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, China)
Abstract: Fuji apple bud mutation and the wild type were analyzed by SSAP. The results showed that a DNA fragment of 376 bp in length was specific fragment of Fuji apple bud mutation, and it was up to 90% homology with the promoter of chalcone synthase(CHS) gene. It indicated that Fuji bud mutation was stable mutation of the genetic material changes, and laid a basis for further research on the molecular mechanism of bud mutation.
Key words: apple(Malus domestica); bud mutation; SSAP; analysis