基于轉錄組學測序的長白落葉松材性表達基因1)

趙佳麗　張磊　張素芳　王艷紅　張含國

(林木遺傳育種國家重點實驗室(東北林業大學)，哈爾濱，150040)

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基于轉錄組學測序的長白落葉松材性表達基因1)

趙佳麗張磊張素芳王艷紅張含國

(林木遺傳育種國家重點實驗室(東北林業大學)，哈爾濱，150040)

摘要基于邊合成邊測序(Sequencing By Synthesis，SBS)技術，使用Illumina HiSeq2500高通量測序平臺對長白落葉松cDNA文庫進行測序。共獲得非重復序列基因58 683條，總長度為55 283 938 bp。將得到的轉錄本使用BLAST軟件將非重復序列基因序列與NR、Swiss-Prot、GO、COG、KEGG數據庫比對，通過選擇BLAST參數E-value不大于10(-5)，最終獲得29 350個有注釋信息的基因序列，其中19 292個轉錄本注釋GO編號，有9 112個轉錄本在COG數據庫中被注釋并分為25個功能分類，同時注釋到120條KEGG代謝途徑。在得到注釋的轉錄本中搜索到木質素相關基因共68條，其中與木質素合成相關基因29條，與木質素分解代謝相關基因27條；找到了147條纖維素相關基因，其中與纖維素合成相關基因103條，與纖維素分解代謝相關基因44條。

關鍵詞長白落葉松；轉錄組；高通量測序；材性基因

分類號S791.225

Gene Expression of Wood Properties Based on Transcriptome ofLarixolgensis

Zhao Jiali, Zhang Lei, Zhang Sufang, Wang Yanhong, Zhang Hanguo

(State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, P. R. China)//Journal of Northeast Forestry University，2016,44(4)：8-12.

Based on sequencing-by-synthesis (Sequencing By Synthesis, SBS) technology, we used Illumina HiSeq2500 high-throughput sequencing to perform the cDNA libraryLarixolgensisfor sequencing platform. We acquired 58 683 Unigenes, and the total length was 55 283 938 bp. We used BLAST software to compare Unigenes sequence of transcript with NR, Swiss-Prot, GO, COG, KEGG database.The parameter of E-value was not more than 10-5with 29 350 annotated Unigenes. Among them, 19 292 annotations was from GO, 9 112 from COG, and these Unigenes was divided into 25 functional categories, 120 from KEGG. There were 68 Unigenes connected with lignin biosynthesis in the annotated transcripts. The Unigenes related to lignin biosynthesis was 29, and the Unigenes related to lignin catabolism was 27. We found 147 Unigenes related to cellulose. The Unigenes related to cellulose biosynthesis was 103, and the Unigenes related to cellulose catabolism was 44.

KeywordsLarixolgensis； Transcriptome； High throughput sequencing； Wood properties

轉錄組(Transcriptome)[1]廣義上指在特定環境或生理條件下的一個細胞、組織或生物體中所有表達基因的總和。轉錄組學是一門在整體水平上研究某一時刻某一細胞中基因全部轉錄本種類、結構和功能及轉錄調控規律的學科，其目的在于提供構成生物全部基因的表達調節系統和全部蛋白質的功能、相互作用等信息，以及解析生物細胞功能的全部情況[2]。隨著后基因組時代的到來，轉錄組學是最先發展并且應用最廣泛的技術[3]?！?br>