柚皮素對去卵巢所致大鼠骨質疏松癥的影響

吳新濤　石晶　高樂才　孫國昌　吳文元　龐石磊

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·論著·

柚皮素對去卵巢所致大鼠骨質疏松癥的影響

吳新濤石晶高樂才孫國昌吳文元龐石磊

目的觀察柚皮素對去卵巢所致大鼠骨質疏松癥的治療作用。方法50只雌性SD大鼠隨機分為假手術組、模型組、柚皮素低、中、高劑量組，每組10只。假手術組施行假手術，其余4組均行卵巢摘除術，術后4周開始灌胃給予35、70、140 mg/kg柚皮素。12周后分別檢測5組大鼠血清雌二醇(E2)、孕激素(P)、抗酒石酸酸性磷酸酶(TRACP)、堿性磷酸酶(ALP)、骨鈣素(BGP)，尿液鈣(Ca)、磷(P)及脫氧吡啶啉(DPD)、骨密度(GMD)、股骨三點彎曲應力、L5腰椎抗壓縮載荷及骨小梁形態變化。結果與假手術組比較，模型組大鼠體重明顯增加，子宮臟器系數明顯降低，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠體重較模型組明顯降低，子宮臟器系數較模型組明顯增加，差異有統計學意義(P<0.05)，并呈劑量依賴性。與假手術組比較，模型組大鼠血清E2、P明顯降低，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠血清E2、P較模型組明顯增加，差異有統計學意義(P<0.05)，呈劑量依賴性。與假手術組比較，模型組大鼠血清TRACP及尿液Ca、P、DPD明顯增加，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組血清TRACP及尿液Ca、P、DPD較模型組明顯降低，差異有統計學意義(P<0.05)，且呈劑量依賴性。與假手術組比較，模型組大鼠血清ALP明顯增加，BGP明顯降低，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠血清ALP較模型組明顯降低，BGP較模型組明顯增加，差異有統計學意義(P<0.05)，呈劑量依賴性。與假手術組比較，模型組大鼠BMD明顯降低，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠BMD較模型組明顯增加，差異有統計學意義(P<0.05)，呈劑量依賴性。與假手術組比較，模型組大鼠股骨三點彎曲應力、L5腰椎抗壓縮載荷明顯降低，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠股骨三點彎曲應力、L5腰椎抗壓縮載荷較模型組明顯增加，差異有統計學意義(P<0.05)，呈劑量依賴性。與假手術組比較，模型組大鼠骨小梁面積百分數、骨小梁數、骨小梁厚度明顯降低，骨小梁分離度明顯增加，差異有統計學意義(P<0.05)；柚皮素低、中、高劑量組大鼠骨小梁面積百分數、骨小梁數、骨小梁厚度較模型組明顯增加，骨小梁分離度較模型組明顯降低，差異有統計學意義(P<0.05)，呈劑量依賴性。結論柚皮素對去卵巢所致大鼠骨質疏松癥具有良好的治療作用。

柚皮素；去卵巢；骨質疏松

model group were obviously increased, however, the seum levels of BGP were significantly decreased (P<0.05).The serum levels of ALP in the three medication groups were significantly decreased,however,the serum levels of BGP were significantly increased,as compared with those in model group (P<0.05),with a dose-dependent manner. As compared with that in sham-operation group,the BMD was significantly decreased in model group (P<0.05).The levels of BMD in the three medication groups were significantly increased, as compared with those in model group (P<0.05), with a dose-dependent manner. As compared with those in sham-operation group,the maximum bending stress and loading force of the 5th lumbar vertebra in model group were significantly decreased (P<0.05). The maximum bending stress and loading force of the 5th lumbar vertebra in low-dose,median-dose and high-dose naringenin groups were significantly increased, as compared with those in model group (P<0.05),with a dose-dependent manner. As compared with those in sham-operation group,the percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula were significantly increased in model group (P<0.05). The percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula in the three medication groups were significantly increased, as compared with those in model group (P<0.05),however the resolution of bone trabecula area in the three medication groups was significantly decreased (P<0.05), with a dose-dependent manner.ConclusionNaringenin has better therapeutic effects on ovariectomy-induced osteoporosis in rats.

骨質疏松癥是好發于絕經后女性、老年人和慢性疾病患者的常見病、多發病，發病原因是由于全身骨量減少、骨微細結構退化導致骨脆性及骨折風險增加。近年來，骨質疏松癥發病率逐漸升高，我國60歲以上的老年人中約80%患有不同程度的骨質疏松癥，其中以女性患者為主[1]。目前，臨床用于預防和治療骨質疏松癥的藥物主要有雌激素受體調節劑、雙膦酸鹽類、降鈣素、氟制劑等，雖然取得了一定療效，但長期服藥不良反應大，且價格昂貴[2]。因此，尋找安全有效的防治骨質疏松癥的藥物已經成為當前的研究熱點。中草藥柚皮苷屬黃酮類物質，具有抗氧化、保護神經、治療骨質疏松癥等藥理作用。柚皮素是柚皮苷的主要代謝產物，體外實驗表明柚皮素對大鼠成骨細胞促骨形成活性要優于柚皮苷[3,4]。……