轉(zhuǎn)化生長(zhǎng)因子—β3與纖維連接蛋白在子宮肌瘤及子宮肌層內(nèi)的表達(dá)水平與生物學(xué)意義

潘偉 汪海霞 劉霞

【摘要】目的：探討轉(zhuǎn)化生長(zhǎng)因子（TGF）-β3與纖維連接蛋白（FN）在絕經(jīng)或未絕經(jīng)婦女子宮肌瘤及子宮肌層中表達(dá)的水平變化及其意義。方法：采用免疫組化法（SP）檢測(cè)40例絕經(jīng)后子宮肌瘤婦女（絕經(jīng)組）、40例未絕經(jīng)子宮肌瘤婦女（未絕經(jīng)組）肌瘤組織及肌壁組織中的TGF-β3、FN的表達(dá)差異，同時(shí)將絕經(jīng)組婦女根據(jù)肌瘤是否發(fā)生萎縮分為萎縮組（17例）、未萎縮組（23例）并進(jìn)行上述指標(biāo)的比較。結(jié)果：絕經(jīng)組的肌瘤組織、肌壁組織中的TGF-β3表達(dá)評(píng)分分別為（3.11±0.78）分、（1.85±0.69）分均顯著的低于未絕經(jīng)婦女的（3.98±0.67）分、（2.31±0.82）分（P<0.05）；兩組婦女肌瘤組織和肌壁組織中的FN表達(dá)評(píng)分差異不顯著（P>0.05）。子宮肌瘤萎縮和未萎縮患者的子宮肌瘤組織、肌壁組織中TGF-β3、FN表達(dá)在兩組間差異均不顯著（P>0.05），萎縮組和未萎縮組的肌瘤組織TGF-β3表達(dá)分別為（2.90±0.65）分、（3.32±0.74）分均顯著的高于本組肌壁組織中的（1.85±0.67）分、（2.25±0.61）分（P<0.05）；兩組肌瘤和肌壁組織中的FN表達(dá)差異不顯著（P>0.05）。結(jié)論：TGF-β3表達(dá)可能與患者絕經(jīng)前后的激素水平改變有關(guān)。

【關(guān)鍵詞】轉(zhuǎn)化生長(zhǎng)因子-β3；纖維連接蛋白；絕經(jīng)；子宮肌瘤；子宮肌層

Expression and biological significance oftransforming growth factor-β3 and fibronectin in uterine myoma and uterine muscle layerPAN Wei1， WANG Haixia2， LIU Xia2. 1. Department of Gynecology， Huangshi Central Hospital， Huangshi 435000， Hubei， China； 2. Department of Obstetrics， Huangshi AiKang Hospital， Huangshi 435000， Hubei， China

【Abstract】Objectives： To discuss the expression and biological significance of transforming growth factor-β3 and fibronectin in uterine myoma and uterine muscle layer. Methods： Using immunohistochemical method （SP）， 40 cases of postmenopausal uterine leiomyoma （postmenopausal group） and 40 cases of premenopausal uterine leiomyoma （premenopausal group） were selected. Different expression of TGF- beta-3 and NF on leiomyoma tissue and muscle tissues were detected. At the same time， postmenopausal group was divided into atrophy group （17 cases） and non-atrophy group （23 cases）. The above indexes of the two groups were compared. Results： The TGF- beta-3 expression scores of premenopausal group in muscle tissue and leiomyoma wall were （3.11±0.78） and （1.85± 0.69） points， which were significantly lower than these of premenopausal group （3.98 ± 0.67， 2.31 ± 0.82） （P<0.05）； FN expression score of two groups in leiomyoma tissue and muscle tissues had no significant difference （P>0.05）. TGF- beta-3 and FN expression of atrophy group and non-atrophy group in uterine fibroids and uterine tissues had no significant difference （P>0.05）. TGF- beta-3 expression of atrophy group and non-atrophy group in uterine fibroids were （2.90±0.65） and （3.32±0.74）， which were both significantly higher than these in uterine tissues of （1.85± 0.67） and （2.25± 0.61） （P<0.05）； FN expression of the two group in fibroids and muscle tissues did not show significant difference （P>0.05）. Conclusion： TGF- beta-3 expression of premenopausal patients is significantly higher than that of postmenopausal patients； TGF- beta-3 expression of atrophic group and non-atrophy patients in leiomyoma is higher than that in muscle wall tissue， without significant difference. TGF- beta-3 expression may be associated with hormone level changes in postmenopausal patients.

【Key words】Transforming growth factor beta-3； Fibronectin； Menopause； Uterine myoma； Uterine muscular layer

【中圖分類號(hào)】R737.33【文獻(xiàn)標(biāo)志碼】A

子宮肌瘤作為女性生殖系統(tǒng)常見(jiàn)的良性腫瘤，其發(fā)病率約占全部女性生殖系統(tǒng)疾病的25%～30%，是導(dǎo)致女性子宮全切的重要原因。其發(fā)病機(jī)制與卵巢分泌的雌、孕激素對(duì)局部生長(zhǎng)因子的自分泌和旁分泌調(diào)節(jié)，從而影響肌瘤細(xì)胞的生長(zhǎng)和分化有關(guān)[1]。轉(zhuǎn)化生長(zhǎng)因子-β3（transforming growth factor-β3， TGF-β3）與纖維連接蛋白（fibronectin ，F(xiàn)N）等多種生產(chǎn)因子均會(huì)對(duì)子宮肌瘤的生長(zhǎng)、發(fā)展產(chǎn)生重要作用，從而出現(xiàn)肌細(xì)胞異常增殖以及細(xì)胞外基質(zhì)聚集等[2，3]。為此，本研究針對(duì)TGF-β3以及FN在絕經(jīng)或未絕經(jīng)婦女子宮肌瘤及子宮肌層中表達(dá)的水平變化及其意義進(jìn)行探討。現(xiàn)將……