不同誘導子對油樟內生菌次生代謝產物的影響

汪超 譚韻雅 楊勝偉

摘要[目的]提高油樟內生菌次生代謝產物的積累。[方法] 將不同誘導子（油樟油水濁液、油樟葉水提液和油樟愈傷組織）添加到油樟內生菌（YY26、YG42）的培養體系中，研究其對內生菌生長及其次生代謝產物（1，8-桉葉油素、松油烯-4-醇、α-松油醇）的影響。[結果]從內生菌生長情況來看，油樟愈傷組織對內生菌YY26的生長有明顯抑制作用，而對YG42的生長有促進作用；油樟油水濁液對2種內生菌的生長都有一定的抑制作用；油樟葉水提液對2種內生菌的生長均無顯著影響。從次生代謝產物積累來看，油樟葉水提液對2種內生菌產1，8-桉葉油素、松油烯-4-醇及α-松油醇具有較強的促進作用，且對1，8-桉葉油素的促進作用最強。當油樟葉水提液濃度為1.00%時，內生菌YY26的1，8-桉葉油素產量最高，比對照組提高了11.80倍；當油樟葉水提液濃度為2.50%時，內生菌YG42中的1，8-桉葉油素產量達到最大值，與對照組相比增加了7.73倍。油樟愈傷組織對總揮發性物質的促進作用最強，當其濃度為10.00%時，對內生菌YY26總揮發性物質的促進作用最強，比對照組提高了13.26倍。[結論]該研究在一定程度上提高了油樟內生菌（YY26、YG42）次生代謝產物的積累量，為其今后的工業化生產奠定了基礎。

關鍵詞油樟；內生菌；1，8-桉葉油素；松油烯-4-醇；α-松油醇

中圖分類號Q942文獻標識碼A文章編號0517-6611（2017）20-0014-04

Abstract[Objective] To improve the accumulation of secondary metabolites of endophytic fungi of Cinnamomum longepaniculatum. [Method] Different inducers（oil， water extract and callus from C. longepaniculatum，were added in the culture system of endophytic fungi（YY26，YG42）of C. longepaniculatum to study their effects on the growth of endophytic fungi and their secondary metabolites（1，8eucalyptol， terpinen4ol， αterpineol）. [Result] As for the growth of endophytic fungi， callus from C. longepaniculatum had obvious inhibitory effects on the growth of YY26，but it had promoting effects on the growth of YG42. Oil from C. longepaniculatum had some inhibiting effects on the growth of YY26 and YG42. Water extracts from C.longepaniculatum had no significant effect on the growth of YY26 and YG42. As for the accumulation of secondary metabolites，water extract from C.longepaniculatum had stronger promoting effects on the yield of 1，8 cineole， terpinen4ol and αterpineol of YY26 and YG42， and it had the strongest promoting effects on the yield of 1，8 cineole.When the concentration of water extract was 1.00%，the yield of 1，8cineole of YY26 was the highest， which was increased than that of control group by 11.80 times. When the concentration of water extract was 2.50%， the yield of 1，8cineole of YG42 was the highest， which was increased than that of control group by 7.73 times.Callus from C.longepaniculatum had the strongest promoting effects on total volatile of secondary metabolites. When the concentration of callus from C.longepaniculatum was 10.00%，it had the strongest promoting effects on total volatile substances of YY26， which was increased than that of control group by 13.26 times. [Conclusion]The accumulation amount of secondary metabolites of YY26 and YG42 in C.longepaniculatum were improved， which can lay a foundation for its industrialized production in the future .

Key wordsCinnamomum longepaniculatum；Endophytic fungi；1，8cineole；Terpinen4ol；αterpineol

植物內生菌在長期的進化過程中，與宿主細胞形成了互利共生的友好關系。目前，有關內生菌生物學作用的研究報道逐年增多，尤其是關于內生菌次級代謝產物積累的研究已成為熱點[1-2]。……