唾液酸酶法檢測細菌性陰道病的診斷價值

王君

摘 要：目的 探討唾液酸酶法檢測細菌性陰道病的診斷價值。方法 選取2017年6月～2018年5月在本院婦科門診疑似細菌性陰道病1073例患者，同時對其給予唾液酸酶法和Amsel法，比較這兩種診斷方法對細菌性陰道病患者的診斷結(jié)果，并對其臨床診斷價值進行分析。結(jié)果 唾液酸酶法與Amsel法對細菌性陰道病的確診率相似，差異無統(tǒng)計學意義（P<0.05）。用Amsel法做參照，唾液酸酶法檢測細菌性陰道病的敏感性為88.60%，特異性為95.15%。結(jié)論 唾液酸酶法可更加客觀的區(qū)分將細菌性陰道病的陽性和陰性，并且敏感性、特異性和總符合率均較高，可以作為檢測該病的有效輔助診斷方法。

關(guān)鍵詞：唾液酸酶法；Amsel法；細菌性陰道病

中圖分類號：R711.3 文獻標識碼：A DOI：10.3969/j.issn.1006-1959.2018.22.049

文章編號：1006-1959（2018）22-0164-03

Diagnostic Value of Sialidase Method for Bacterial Vaginosis

WANG Jun

（Department of Clinical Laboratory， the Second Affiliated Hospital of Shantou University Medical College，Shantou 515041，Guangdong，China）

Abstract：Objective To investigate the diagnostic value of sialidase method in detection of bacterial vaginosis.Methods From June 2017 to May 2018， 1073 patients with suspected bacterial vaginosis were selected from gynecological outpatient department of our hospital. They were given sialidase method and Amsel method to compare the diagnostic results of these two methods for patients with bacterial vaginosis. And its clinical diagnostic value was analyzed.Results The sialidase method and the Amsel method were similar in the diagnosis rate of bacterial vaginosis， and the difference was not statistically significant （P<0.05）. Using the Amsel method as a reference， the sensitivity of the sialidase assay for detecting bacterial vaginosis was 88.60% and the specificity was 95.15%. Conclusion The sialidase method can distinguish the positive and negative of bacterial vaginosis more objectively， and the sensitivity， specificity and total coincidence rate are higher， which can be used as an effective auxiliary diagnosis method for detecting the disease.

Key words：Sialidase method；Amsel method；Bacterial vaginosis

細菌性陰道病（bacterial vaginosis，BV）是由于陰道正常菌群生態(tài)平衡失調(diào)的一種臨床病征，主要是陰道內(nèi)正常的乳酸桿菌減少而導(dǎo)致其他病原如加德納菌、各種厭氧菌、彎曲弧菌等的大量繁殖[1]。其診斷主要靠實驗室診斷，目前本院門診開展的細菌性陰道病的檢驗方法有傳統(tǒng)的Amsel法和新開展的唾液酸酶法。現(xiàn)對本院婦科門診疑似感染細菌性陰道病就診的女性患者同時進行兩種方法檢驗，探討唾液酸酶法檢測診斷細菌性陰道病的臨床診斷價值。

1資料與方法

1.1一般資料 標本均來自2017年6月～2018年5月婦科門診就診的疑似BV的患者，共1073例，年齡為18～61歲，平均年齡（32.20±3.35）歲。

1.2標本采集 由婦產(chǎn)科醫(yī)生按照臨床操作要求規(guī)范取材：用專用拭子從陰道側(cè)壁或后穹隆處取分泌物，及時送至門診檢驗科進行唾液酸酶法和Amsel法檢測。標本要求：①標本采集時應(yīng)處于非月經(jīng)期，采集前7 d內(nèi)未使用抗菌藥物，前3 d未使用陰道洗劑或無性生活。②標本采集后及時送檢，防止采樣拭子干燥。

1.3儀器與試劑 BVBLUE 及……