基于cAMP信號通路研究miR-7a2對青春期大鼠雄性生殖系統(tǒng)發(fā)育的影響

徐瑋 杜二球

[摘要] 目的 研究基于環(huán)磷酸腺苷（cAMP）信號通路分析微小RNA-7a2（miR-7a2）對青春期大鼠雄性生殖系統(tǒng)發(fā)育的影響。 方法 本研究選取2020年3—10月健康、清潔級Wistar雄性鼠齡3周大鼠30只，隨機分為對照組（生理鹽水）、miR-7a2上調(diào)組（超表達(dá)pmcherry-Flag-miR-7a2）、miR-7a2下調(diào)組（慢病毒質(zhì)粒miR-7a2-sh RNA-1），每組各10只。檢測三組激素水平，統(tǒng)計體重、睪丸重量、精子畸變、活動度，觀察睪丸形態(tài)學(xué)，檢測cAMP信號通路相關(guān)蛋白腺苷酸環(huán)化酶（AC）、磷酸二酯酶（PDE）、蛋白激酶（PK）。 結(jié)果 miR-7a2上調(diào)組miR-7a2表達(dá)高于對照組和miR-7a2下調(diào)組;miR-7a2下調(diào)組miR-7a2表達(dá)低于對照組（P<0.05）。miR-7a2上調(diào)組雌二醇、卵泡刺激素水平低于miR-7a2下調(diào)組;睪酮水平高于miR-7a2下調(diào)組（P<0.05）。miR-7a2上調(diào)組體重、睪丸重量、精子密度高于miR-7a2下調(diào)組，精子畸變率低于miR-7a2下調(diào)組（P<0.05）。miR-7a2上調(diào)組良好、一般精子活動度高于miR-7a2下調(diào)組;不良、死亡精子活動度低于miR-7a2下調(diào)組（P<0.05）。miR-7a2上調(diào)組AC蛋白表達(dá)低于miR-7a2下調(diào)組，PK蛋白表達(dá)高于miR-7a2下調(diào)組（P<0.05）。 結(jié)論 miR-7a2上調(diào)能促進青春期大鼠雄性生殖系統(tǒng)發(fā)育，其作用機制與cAMP信號通路有關(guān)。

[關(guān)鍵詞] 環(huán)磷酸腺苷;微小RNA-7a2;青春期;生殖系統(tǒng)發(fā)育

[中圖分類號] R691.5? ? ? ? ? [文獻標(biāo)識碼] A? ? ? ? ? [文章編號] 1673-9701（2021）24-0045-05

Effect of miR-7a2 on the development of the male reproductive system in adolescent rats based on the cAMP signaling pathway

XU Wei? ?DU Erqiu

Department of Gynecology， Taizhou Central Hospital in Zhejiang Province （Taizhou University Hospital）， Taizhou? ?318000， China

[Abstract] Objective To investigate the effect of microRNA-7a2 （miR-7a2） on the development of the male reproductive system in adolescent rats based on the cyclic adenosine monophosphate （cAMP） signaling pathway. Methods Thirty healthy， clean Wistar male rats aged three weeks from to March to October 2020 were randomly divided into the control group （saline）， the miR-7a2 up-regulation group （overexpression of pmcherry-Flag-miR-7a2）， and miR-7a2 down-regulation group （lentiviral plasmid miR-7a2-shRNA-1）， with ten rats in each group. Hormone levels were detected. The body weight， testicular weight， sperm aberrations， and motility were measured. The testicular morphology was observed. The cAMP signaling pathway-related proteins adenylate cyclase （AC）， phosphodiesterase （PDE）， and protein kinase （PK） were measured. Results miR-7a2 expression was higher in the miR-7a2 up-regulation group than in the control and miR-7a2 down-regulation groups. miR-7a2 expression was lower in the miR-7a2 down-regulation group than in the control group（P<0.05）. Estradiol and follicle-stimulating hormone levels were lower， and testosterone levels were higher in the miR-7a2 up-regulation group than in the miR-7a2 down-regulation group（P<0.05）. Body weight， testicular weight， and sperm density were higher， and sperm aberration rate was lower in the miR-7a2 up-regulation group than in the miR-7a2 down-regulation group（P<0.05）.The excellent and general sperm motility in the miR-7a2 up-regulation group was higher than that in the miR-7a2 down-regulation group. The poor and dead sperm motility in the miR-7a2 up-regulation was lower than in the miR-7a2 down-regulation group（P<0.05）. AC protein expression was lower， and PK protein expression was higher in the miR-7a2 up-regulation group than in the miR-7a2 down-regulation group（P<0.05）. Conclusion The up-regulation of miR-7a2 can promote the development of the male reproductive system in adolescent rats， and the mechanism is related to the cAMP signaling pathway.