雷公藤甲素對哮喘小鼠白三烯B₄\\C₄表達的影響

【摘要】 目的 研究雷公藤甲素對哮喘小鼠哮喘小鼠白三烯B₄、C₄表達的影響，探討其治療哮喘的機制。方法 建立小鼠卵蛋白哮喘模型，隨機分為7組，分別為對照組、地塞米松治療組及不同劑量雷公藤甲素治療組，用ELISA測定支氣管肺泡灌洗液(BALF)及肺組織勻漿中白三烯 B₄、C₄表達水平。結果 哮喘組白三烯 B₄、C₄表達明顯高于正常對照組(P<0.01)，雷公藤甲素治療組及地塞米松組明顯低于哮喘組(P<0.01)。結論 雷公藤甲素抑制哮喘氣道炎癥的機制可能與其抑制白三烯B₄、C₄表達的表達活性有關。

【關鍵詞】 雷公藤甲素;支氣管哮喘;LTB₄;LTC₄

文章編號:1003-1383(2009)05-0516-02

中圖分類號:R 562.25;R363文獻標識碼:A

doi:10.3969/j.issn.1003-1383.2009.05.002

Effects of triptolide on the expression of TLB₄ and TLC₄ in asthmatic mice[HJ1*2][HJ]

LIU Qingyi

(Respiratory Yuebei People’s Hospital Guangdong，Shaoguan 512026)

【Abstract】 Objective To study the effect s of triptolide on the expression of TLB₄ and TLC₄ in asthmatic mouse model，and to explore the possible therapeutic mechanism of t riptolide in asthma.

Methods The asthmatic model was established by ovalbumin injection and inhalation. The mice were randomly divided into 7 groups andtreated respectively with normal saline (as control) ，dexamethasone or different dose of triptolide. The expression of TLB₄ and TLC₄ in ranchoalveolar avage fluid (BAL F) and in lung tissue was were detected by ELISA respectively.Results The expression of TLB₄ and TLC₄ in airway epithelium of asthmatic group significantly increased compared with those of controlgroup(P<0.01). The expression of TLB₄ and TLC₄ expression in mice t reated with different dose of t riptolide and dexamethson were significantly lower than those in asthmatic group(P<0.01).Con

clusion The mechanism of t riptolide inhibiting asthmatic airway inflammation may be related to its inhibiting activities of TLB₄ and TLC₄.

【Key words】 Triptolide;Asthma;LTB₄;TLC₄

支氣管哮喘是一種常見病、多發病，由于對其病因及發病機制不甚清楚，雖然采用現有的方法，已能有效地控制大多數病人的癥狀，但對部分病人療效欠佳，尋求和開發有效的治療哮喘的藥物對哮喘的防治具有十分重要的意義。本實驗研究雷公藤甲素對哮喘小鼠白三烯B₄、C₄表達的影響，為開發雷公藤甲素治療哮喘在臨床上應用提供理論依據。