大豆蛋白水解物促乳酸菌增殖發酵的研究

白鳳翎，曲玲童，張柏林，趙宏飛

（1.渤海大學化學化工與食品安全學院，遼寧省食品安全重點實驗室，“食品貯藏加工及質量安全控制工程技術研究中心”遼寧省高校重大科技平臺，遼寧錦州121013；2.北京林業大學生物科學與技術學院，北京 100083）

大豆蛋白水解物促乳酸菌增殖發酵的研究

白鳳翎1，曲玲童1，張柏林2，趙宏飛2

（1.渤海大學化學化工與食品安全學院，遼寧省食品安全重點實驗室，“食品貯藏加工及質量安全控制工程技術研究中心”遼寧省高校重大科技平臺，遼寧錦州121013；2.北京林業大學生物科學與技術學院，北京 100083）

應用小型生物發酵罐對乳酸菌增殖作用進行發酵研究，結果表明，12g/100mL脫脂乳中添加大豆蛋白水解物增殖復合劑對嗜熱鏈球菌和保加利亞乳桿菌生長具有顯著的促進作用。添加酵母膏脫脂乳培養基的產酸量比脫脂乳增加1倍，添加增殖復合劑后為脫脂乳的3.12倍。添加增殖復合劑可使乳酸菌數量達到10個數量級，比脫脂乳培養基提高1～2個數量級。從累積添加堿量和單位加堿量兩項指標來看，脫脂乳培養基乳酸菌生長呈現緩慢上升曲線，添加酵母膏呈現三段波浪式曲線，添加增殖復合劑為陡峭的上升曲線。單純添加酵母膏不能支持乳酸菌連續生長，添加大豆蛋白水解物和酵母膏能夠使乳酸菌生長快速啟動，并維持一個較高生長水平，獲得較大的細胞量的同時也充分利用乳中蛋白質。

大豆蛋白水解物，乳酸菌，發酵，增殖

Abstract：The fermentation tests of proliferation on lactic acid bacteria（LAB） was carried out in bioreactor，the results showed that growth of Streptococcus thermophilus and Lactobacillus bulgaricus were significantly promoted in 12g/100mL skim milk supplemented with soy protein hydrolysates（SPH）.The acid production of LAB on nonfat milk supplemented with yeast extracts and multiplication complex were 2 and 3.12 times than that of control.Then the cell count of LAB reached to 10lg cycle on skim milk supplemented with multiplication complex，which increased 1.0～2.0lg cycle than control.The parameter of accumulate additional alkali volume and interval additional alkali volume showed that the growth curve of LAB on skim milk ascend slowly，and three waves trend as supplemented with yeast extract，and went up dramatically with addition of multiplication complex.The addition of yeast extract alone can not support the continual growth of LAB，however，the addition of yeast extract and protein hydrolytes together can accelerate it and made it to keep in a high growth level.Much more biomass of LAB on milk with multiplication complex would be obtained and the protein in milk would be utilized sufficiently.

Key words：soy protein hydrolysates；lactic acid bacteria；fermentation；proliferation

直投式（direct-vat-set，DVS）酸奶發酵劑具有無需中間繼代培養，使用方便快捷，產品質量穩定等特點，在發酵乳制品中廣泛應用，作為一類新型的商品化制劑，細胞數量是影響乳酸菌發酵性能的關鍵因素。……