一氧化氮對胎兔動脈導管胱硫醚—γ—裂解酶/硫化氫體系的影響

摘要：目的 探討H2S、NO對早產胎兔動脈導管的作用及其相互影響，進一步探索動脈導管開放與關閉的病理生理機制、意義及其影響因素，為臨床上全面認識動脈導管的開放與關閉提供理論依據。方法 將孕21d日本大耳白兔24只隨機分成四組：空白對照組及三個處理組，處理組待孕齡23d和25d時分別腹腔注射以不同劑量的一氧化氮前體-硝普鈉，待孕齡26d時解剖孕兔取胎兔，胎兔心臟取血1mL后立即解剖胎兔取動脈導管組織，進行實驗。結果 隨著注射硝普鈉濃度的增加，胎兔血漿中硫化氫含量、動脈導管組織CSEmRNA表達量均逐漸隨注射劑量的增加而增加。各組間相比較，P<0.01，具有顯著性差異。結論 早產兔血漿中有H2S存在，早產兔動脈導管組織中存在CSEmRNA表達；給孕兔注射不同劑量硝普鈉（在一定范圍內）對早產兔動脈導管中H2S/CSE體系具有濃度依賴性促進作用。

關鍵詞：動脈導管；早產兔；一氧化氮；硫化氫；胱硫醚-γ-裂解酶

Abstract： Objective To observe nitric oxide and hydrogen sulfide on ductus arteriosus in preterm fetal rabbits the role and influence each other， to further explore the pathophysiology of arterial catheter opening and closing mechanism， significance and impact factors， a comprehensive understanding of clinical arterial catheter provides a theoretical basis. Methods For Pregnant 21 days of 24 Japanese white rabbits were randomly divided into four groups： control group and three treatment groups， until 23 days and 25 days gestational age were more than intrauterine dose of sodium nitroprusside injection.Anatomy of gestational age until 26 days pregnant rabbit rabbit fetus， fetal 1mL Rabbit heart blood taken immediately after the arterial anatomy of fetal tissue in rabbits，and the experiment was conducted. Results Preterm fetal rabbits plasma hydrogen sulfide content and the expression levels of CSEmRNA， increased gradually withl injection of increasing doses of sodium nitroprusside. Conclusion Hydrogen sulfide in premature rabbit plasma exists ，the expression of CSEmRNA and CSE in preterm rabbit ductus arteriosus is higher than full-term fetal rabbit ； different doses of sodium nitroprusside （within a certain range） of the ductus arteriosus in preterm rabbits in a concentration H2S/CSE pathway dependent role in promoting.

Key words：Ductus arteriosus； Premature rabbits； Nitric oxide；Hydrogen sulfide； Cystathionine-γ-lyase

目前國外學者對動脈導管關閉分子機制的研究表明，動脈導管在宮內本身開放是處于一個主動的狀態，而動脈導管自身具有一定的張力和收縮傾向，但在宮內動脈導管收縮能力被各種擴血管的因素所抑制，出生后由于擴張和收縮動脈導管的各種血管活性因子間的平衡被破壞，使動脈導管傾向于收縮力量一方，于是動脈導管很快便自行關閉。目前對于這些收縮導管及擴張導管的因素及其具體機制并非完全清楚，它們是如何通過各自的獨立作用及之間相互滲透共同控制導管的舒縮的有待進一步研究?！?br>