泥鰍粉對訓(xùn)練小鼠游泳力竭24h后心肌S100A4蛋白表達(dá)的影響

摘要：目的 從心肌S100A4蛋白表達(dá)變化探討生泥鰍干預(yù)對游泳訓(xùn)練力竭小鼠心肌的作用。方法 24只健康雄性ICR小鼠分為安靜組（C）、運(yùn)動(dòng)組（T）、泥鰍運(yùn)動(dòng)組（N），T組和N組游泳訓(xùn)練3w后進(jìn)行力竭運(yùn)動(dòng)，24h后取材檢測血清CK-MB，HE染色光鏡下觀察心肌組織，免疫組化法檢測心肌P53和S100A4蛋白的表達(dá)。結(jié)果 ①N組力竭游泳時(shí)間顯著地長于T組（P<0.01），血清CK-MB活性顯著地低于T組和C組（P<0.05）。②T組的心肌細(xì)胞界限模糊、可見心肌纖維斷裂和間質(zhì)水腫，而N組無明顯病理表現(xiàn)。③與C組比較，T組心肌P53蛋白的表達(dá)顯著增高（P﹤0.05）；S100A4蛋白N組顯著增高（P﹤0.05）。結(jié)論 負(fù)荷游泳訓(xùn)練并力竭24 h后小鼠的心肌細(xì)胞存在損害，生泥鰍干預(yù)能上調(diào)S100A4表達(dá)和減弱P53表達(dá)而降低損害。

關(guān)鍵詞：生泥鰍；力竭運(yùn)動(dòng)；心??；S100A4蛋白

Abstract：Objective This study was to observe effects of raw loach on myocardium by expression of S100A4. Methods 24 healthy male ICR mouse were divided into control group（C）， exercise-trained group（T）and exercise + raw loach group （N）. The exhaustive exercise time of mice was recorded after three week exercise-trained. Activity of serum MB isoenzyme of creatine kinase （CK-MB） was detected. The myocardium were observed by microscope after HE taining.The expressions of S100A4 protein were detected with immunohistochemistry. Results ①The time of exercise exhaustive were longer than group T（P﹤0.01）， the activity of CK-MM were lower than group T and C（P<0.05） in group N. ②There was not obviously tissue damage in group N. ③Compared with group C， the expression of P53 protein increased significantly in group T（P<0.05） and the expression of S100A4 protein was increased significantly in group N（PP<0.05）. Conclusion There was myocardial injury in mice with load swimming training at 24 Hours after exhaustive exercise. But raw loach can reduce the damage of myocardia by increased the expression of S100A4 protein.

Key words：Loach； Exhaustion exercise；Myocardial； S100A4 protein

泥鰍味道鮮美、氨基酸組成全面，必需氨基酸構(gòu)成比例符合FAO/WHO的標(biāo)準(zhǔn)[1]，含有大量益于人體的生物活性物質(zhì)。有研究報(bào)道，泥鰍酶解蛋白、凍干粉和中藥復(fù)合劑有強(qiáng)身保健、抗疲勞和抗自由基保護(hù)機(jī)體作用[1-3]，而對保護(hù)運(yùn)動(dòng)機(jī)體的效果和機(jī)制國內(nèi)外研究極少。眾所周知，力竭運(yùn)動(dòng)力竭運(yùn)動(dòng)對心肌產(chǎn)生損害。近年研究發(fā)現(xiàn)，一種鈣結(jié)合蛋白S100A4能促血管生成和對心臟損傷起保護(hù)作用[4]。本實(shí)驗(yàn)生泥鰍粉應(yīng)用于游泳訓(xùn)練小鼠，觀察力竭游泳能力、心肌組織及心肌S100A4蛋白表達(dá)變化，探索泥鰍保護(hù)運(yùn)動(dòng)機(jī)體心肌的作用與機(jī)制。

1資料與方法

1.1一般資料 健康雄性ICR小鼠，體重（20±2）g，購自湖南斯耐克景達(dá)實(shí)驗(yàn)動(dòng)物有限公司，許可證號SCXK（湘）2011-0003。以國家標(biāo)準(zhǔn)嚙齒類動(dòng)物飼料分籠飼養(yǎng)，自由飲食和飲水?！?br>