培土生金法對肺脾兩虛型慢阻肺大鼠肺功能的影響

[摘要] 目的 探討培土生金法對肺脾兩虛型COPD大鼠肺功能影響。方法 于2016年6—7月，在甘肅中醫學院動物實驗中心開展研究。采用每日被動煙熏、氣管內兩次注射脂多糖及灌服番瀉葉的方法，復制大鼠肺脾兩虛型COPD模型；實驗隨機分為正常組、模型組及治療組，在第28天做大鼠肺功能測定，光鏡觀察大鼠肺組織病理變化。結果 與正常組比較，模型組MV（98.99±0.74）mL、PIF（8.11±0.21）mL/s、PEF（8.55±0.32）mL/s均明顯降低；MV、PEF（P<0.05），PIF（P<0.01），差異有統計學意義。與模型組比，治療組MV（118.17±6.64）mL、PIF（9.05±0.56）mL/s、PEF（9.88±0.82）mL/s均升高；PIF、PEF（P<0.05），MV（P<0.01），差異有統計學意義。模型組大鼠支氣管壁及周圍組織大量炎性細胞浸潤，肺泡壁變薄或斷裂，部分肺泡融合形成肺大皰；治療組大鼠肺組織損傷明顯減輕。結論 培土生金法可以緩解肺脾兩虛型COPD大鼠呼吸系統及全身癥狀，改善肺功能。

[關鍵詞] 培土生金；慢性阻塞性肺疾病；肺脾兩虛型；肺功能

[中圖分類號] R332 [文獻標識碼] A [文章編號] 1674-0742（2016）11（a）-0040-03

[Abstract] Objective To investigate effect of the Method of reinforcing earth to strengthen metal on lung function in pulmonosplenic asthenia rats with COPD.Methods From June to July 2016， the research had been developed in Animal Experimental center. Pulmonosplenic asthenia rats models with COPD were established by forcing rats to smoke every day ，injecting LPS in the airway two times and feeding with decoction of Folium Sennae.Rats were randomized into the normal group， model group and treatment group. In the 28th day，the lung function was measured and the pathologic changes of lung tissue was examined by microscope.Results Compared with the normal group，MV（98.99±0.74）mL、PIF（8.11±0.21）mL/s、PEF（8.55±0.32）mL/s were significantly decreased in the model group，MV、PEF（P<0.05），PIF（P<0.01），the difference had significance of statistics.Compared with the model group，MV（118.17±6.64）mL、PIF（9.05±0.56）mL/s、PEF（9.88±0.82）mL/s were all increased in the treatment group，PIF、PEF（P<0.05），MV（P<0.01），the difference had significance of statistics.In the model group，plentiful inflammatory cells infiltration in the bronchial wall and surrounding tissue，alveolar walls became thin or rupture，some alveolar formed pulmonary bulla.The lung tissue damage In the treatment group decreased obviously.Conclusion The method of reinforcing earth to generate metal could relieve the respiratory system disease and general symptoms， improve lung function in pulmonosplenic asthenia rats with COPD.

[Key words] Reinforcing earth to generate metal；COPD；The Lung and Spleen Deficiency Syndrome；Lung function

慢性阻塞性肺疾病（COPD）以不完全可逆的氣流受限為特征。氣流受限呈進行性發展，伴有氣道和肺對有害顆粒或氣體所致慢性炎癥反應的增加[1]。中醫將其歸于“肺脹”范疇，認為病變首先在肺，繼而影響到脾，中焦脾土的病變是COPD傳變或惡化的關鍵。脾虛則邪易侵，易生痰，肌肉削，進而致疾病纏綿。實驗于2016年6—7月，選取甘肅中醫學院動物實驗中心清潔級Wistar大鼠30只作為研究對象。采用氣管內滴注LPS，被動吸煙及灌服番瀉葉法建立肺脾兩虛型COPD大鼠模型。選取參苓白術散干預治療，觀察其對模型大鼠肺功能和肺組織病理的影響，探討其可能的作用機制，以期為臨床培土生金法治療肺脾兩虛型COPD提供實驗依據。……